Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,190,017	C→A	intergenic (+187/‑103)	ileS → / → SAUSA300_RS05895	isoleucine‑‑tRNA ligase/glyoxalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,190,017	0	C	A	100.0%	43.1 / NA	17	intergenic (+187/‑103)	ileS/SAUSA300_RS05895	isoleucine‑‑tRNA ligase/glyoxalase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/2);  total (15/2)

AAGTTACCGGTCATATATGTAAAAAATGTGCGATTTACTAAAATAAAAATTATTCAGGAATGGTACAAATTCTCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTAAGTAAAAGTAAGCACAGATATTGAATTTGATAGGAGTT  >  NC_007793/1189946‑1190113                                                                        |                                                                                                 aaGTTACCGGTCATATATGTAAAAAATGTGCGATTTACTAAAATAAAAATTATTCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTg                                                                      >  1:3207837/1‑100 (MQ=255)   gTTACCGGTCATATATGTAAAAAATGTGCGATTTACTAAAATAAAAATTATTCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCt                                                                    <  1:5059362/100‑1 (MQ=255)     tACCGGTCATATATGTAAAAAATGTGCGATTTACTAAAATAAAAATTATTCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTAt                                                                  >  1:5239824/1‑100 (MQ=255)            ggtatacGTAAAAAATGTGCGATTTACTAAAATAAAAATTATTCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAAt                                                           >  1:4825445/3‑100 (MQ=255)            cATATATGTAAAAAATGTGCGATTTACTAAAATAAAAATTATTCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAAt                                                           >  1:3970324/1‑100 (MQ=255)                              gCGATTTACTAAAATAAAAATTATTCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAAtttt                                         >  1:1983240/1‑100 (MQ=255)                                   ttACTAAAATAAAAATTATTCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTaagta                                    >  1:2878190/1‑100 (MQ=255)                                     aCTAAAATAAAAATTATTCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTaagtaaa                                  >  1:1619628/1‑100 (MQ=255)                                     aCTAAAATAAAAATTATTCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTaagtaaa                                  >  1:399932/1‑100 (MQ=255)                                     aCTAAAATAAAAATTATTCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTaagtaaa                                  >  1:5588497/1‑100 (MQ=255)                                     aCTAAAATAAAAATTATTCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTaagtaaa                                  >  1:5525003/1‑100 (MQ=255)                                                      tCAGGAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTAAGTAAAAGTAAGCACAGatcgga                 >  1:3884675/1‑96 (MQ=255)                                                       tgggAATGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTAAGTAAAAGTAAGCACAGATATTGa                >  1:3182001/3‑100 (MQ=255)                                                           cctGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTAAGTAAAAGTAAGCACAGATATTGAAttt            >  1:1883871/3‑100 (MQ=255)                                                            aTGGTACAAATTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTAAGTAAAAGTAAGCACAGATATTGAAtttc           >  1:826708/1‑99 (MQ=255)                                                                   aaaTTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTAAGTAAAAGTAAGCACAGATATTGAATTTGATAGGAg    >  1:5265986/1‑100 (MQ=255)                                                                     aTTATCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTAAGTAAAAGTAAGCACAGATATTGAATTTGATAGGAgct  <  1:952294/100‑3 (MQ=255)                                                                        |                                                                                                 AAGTTACCGGTCATATATGTAAAAAATGTGCGATTTACTAAAATAAAAATTATTCAGGAATGGTACAAATTCTCTGAGGCATATAAATGCGTTATAGTTGCTATTCTCAATTATGTTCGCGATAATTTTAAGTAAAAGTAAGCACAGATATTGAATTTGATAGGAGTT  >  NC_007793/1189946‑1190113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑