Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,185,383	C→T	intergenic (+57/‑26)	SAUSA300_RS05875 → / → SAUSA300_RS05880	cell division protein/S4 RNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,185,383	0	C	T	100.0%	92.5 / NA	32	intergenic (+57/‑26)	SAUSA300_RS05875/SAUSA300_RS05880	cell division protein/S4 RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/2);  total (30/2)

TACTCCAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGCATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGAC  >  NC_007793/1185286‑1185469                                                                                                  |                                                                                       tACTCCAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTAt                                                                                      >  1:5361538/1‑100 (MQ=255)     ccAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATCTGTAttttt                                                                                  >  1:547897/1‑100 (MQ=255)     ccAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTAttttt                                                                                  >  1:1226756/1‑100 (MQ=255)     ccAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTAttttt                                                                                  >  1:5573211/1‑100 (MQ=255)             ttAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAatat                                                                          >  1:2914397/1‑100 (MQ=255)             ttAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATACGTATTTTTCTGAatat                                                                          >  1:409890/1‑100 (MQ=255)                     aTTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCaa                                                                  <  1:2222187/100‑1 (MQ=255)                                             aaGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAAttttttt                                          >  1:4968017/1‑100 (MQ=255)                                               gCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTaa                                        >  1:3872420/1‑100 (MQ=255)                                                      aaCGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTg                                 >  1:450186/1‑100 (MQ=255)                                                            gCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAAc                           >  1:1071279/1‑100 (MQ=255)                                                             aaaGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACa                          >  1:3740087/2‑100 (MQ=255)                                                               aGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATa                        >  1:5547695/1‑100 (MQ=255)                                                                 ggTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGa                      >  1:2925311/1‑100 (MQ=255)                                                                      aTGATATTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATAttt                 >  1:3090229/1‑100 (MQ=255)                                                                             tttAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAaca          <  1:3461295/100‑1 (MQ=255)                                                                              ttAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAacac         >  1:1643157/1‑100 (MQ=255)                                                                               tAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAaccat        >  1:1545532/1‑97 (MQ=255)                                                                               tAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAacacc        >  1:2109039/1‑99 (MQ=255)                                                                               tAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAacaca        >  1:2325321/1‑99 (MQ=255)                                                                               tAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACt        >  1:4362461/1‑100 (MQ=255)                                                                               tAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACt        >  1:5153803/1‑100 (MQ=255)                                                                               tAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACt        >  1:2160348/1‑100 (MQ=255)                                                                               tAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACt        >  1:2781181/1‑100 (MQ=255)                                                                                aGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAacacac       >  1:631309/1‑98 (MQ=255)                                                                                aGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAacacac       >  1:5753541/1‑98 (MQ=255)                                                                                aGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACtt       >  1:830161/1‑100 (MQ=255)                                                                                aGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACtt       >  1:1429504/1‑100 (MQ=255)                                                                                aGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACtt       >  1:1752988/1‑100 (MQ=255)                                                                                aGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACtt       >  1:4705762/1‑100 (MQ=255)                                                                                     cGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGat  >  1:679045/1‑99 (MQ=255)                                                                                     cGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGAc  >  1:5442269/1‑100 (MQ=255)                                                                                                  |                                                                                       TACTCCAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGCATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGAC  >  NC_007793/1185286‑1185469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑