Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,168,149	A→G	intergenic (‑115/‑249)	SAUSA300_RS05785 ← / → SAUSA300_RS05790	tRNA‑Arg/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,168,149	0	A	G	100.0%	23.7 / NA	11	intergenic (‑115/‑249)	SAUSA300_RS05785/SAUSA300_RS05790	tRNA‑Arg/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/10);  total (1/10)

AAATTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCAATAAGACTTAAATACGAATTCAGGCAACTTAATTGTGTTAAATACAGTTTTGAATGCCTAACTGTATTTCTTTTCTCTTTAAAATACAG  >  NC_007793/1168075‑1168238                                                                           |                                                                                          aaaTTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGc                                                                  <  1:3803902/100‑1 (MQ=255) aaaTTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGc                                                                  <  1:4943758/100‑1 (MQ=255)  aaTTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGCa                                                                 <  1:2293028/100‑1 (MQ=255)  aaTTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGCa                                                                 <  1:4515571/100‑1 (MQ=255)  aaTTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGATATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGCa                                                                 <  1:228738/100‑1 (MQ=255)   aTTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGCaa                                                                <  1:2853964/100‑1 (MQ=255)   aTTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGCaa                                                                <  1:95924/100‑1 (MQ=255)    ttAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGCAAc                                                               <  1:1013551/100‑1 (MQ=255)      aGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGCAACtt                                                             <  1:1106128/100‑1 (MQ=255)                       aaaaaaTGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGCAACTTAATTGTGTTAAATACAg                                            <  1:1983874/100‑1 (MQ=255)                                                                 aaaaaGGTTCGATAAGACTTAAATACGAATTCAGGCAACTTAATTGTGTTAAATACAGTTTTGAATGCCTAACTGTATTTCTTTTCTCTTTAAAATACAg  >  1:4677141/1‑100 (MQ=255)                                                                           |                                                                                          AAATTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCAATAAGACTTAAATACGAATTCAGGCAACTTAATTGTGTTAAATACAGTTTTGAATGCCTAACTGTATTTCTTTTCTCTTTAAAATACAG  >  NC_007793/1168075‑1168238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑