Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,160,533	G→A	A203V (GCT→GTT)	SAUSA300_RS05750 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,160,533	0	G	A	100.0%	11.8 / NA	6	A203V (GCT→GTT)	SAUSA300_RS05750	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/4);  total (2/4)

CAATTTCTGCATTACGAGGATTTTTAACATAACGGTTTGTGTCAGTTAATTTTAACTTTTTACTTAAATCAATCGTGTAATTATTTCCATCAGCAGTTATCTTAATTTGACCTTTATTAAATTCTCCGTTATATAACTTTTTATTTTTTATTAATGTT  >  NC_007793/1160441‑1160598                                                                                             |                                                                  gatcttCTGCGTTACGAGGATTTTTAACATAACGGTTTGTGTCAGTTAATTTTAACTTTTTACTTAAATCAATCGTGTAATTATTTCCATcaacaggcaa                                                            <  1:302781/96‑5 (MQ=255)    cttCTGCGTTACGAGGATTTTTAACATAACGGTTTGTGTCAGTTAATTTTAACTTTTTACTTAAATCAATCGTGTAATTATTTCCATCAACAGTTATCtt                                                         <  1:2541487/99‑1 (MQ=255)       cTGCATTACGAGGATTTTTAACATAACGGTTTGTGTCAGTTAATTTTAACTTTTTACTTAAATCAATCGTGTAATTATTTCCATCAACAGTTATCTTAAt                                                      >  1:2555988/1‑100 (MQ=255)                gAGGATTTTTAACATAACGGTTTGTGTCAGTTAATTTTAACTTTTTACTTAAATCAATCGTGTAATTATTTCCATCAACAGTTATCTTAATTTGACCttt                                             <  1:4104619/100‑1 (MQ=255)                gAGGATTTTTAACATAACGGTTTGTGACAGTTAATTTTAACTTTTTACTTAAATCAATCGTGTAATTATTTCCATCAACAGTTATCTTAATTTGACCttt                                             <  1:4962843/100‑1 (MQ=255)                                                      caCTTTTTACTTAAATCAATCGTGTAATTATTTCCATCAACAGTTATCTTAATTTGACCTTTATTAAATTCTCCGTTATATAACTTTTTATTTTttatta       >  1:1587054/2‑100 (MQ=255)                                                           tttACTTAAATCAATCGTGTAATTATTTCCATCAACAGTTATCTTAATTTGACCTTTATTAAATTCTCCGTTATATAACTTTTTATTTTTTATTAATGtt  <  1:1030209/100‑1 (MQ=255)                                                                                             |                                                                  CAATTTCTGCATTACGAGGATTTTTAACATAACGGTTTGTGTCAGTTAATTTTAACTTTTTACTTAAATCAATCGTGTAATTATTTCCATCAGCAGTTATCTTAATTTGACCTTTATTAAATTCTCCGTTATATAACTTTTTATTTTTTATTAATGTT  >  NC_007793/1160441‑1160598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑