Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,149,659	G→A	G191D (GGT→GAT)	SAUSA300_RS05655 →	non‑canonical purine NTP pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,149,659	0	G	A	100.0%	229.5 / NA	82	G191D (GGT→GAT)	SAUSA300_RS05655	non‑canonical purine NTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (80/2);  total (80/2)

AATTAGATAAAACCATGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGGTGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCAAATTTATGAAATGCACCCAGATGCAGATGT  >  NC_007793/1149563‑1149753                                                                                                 |                                                                                               aaTTAGATAAAACCATGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGa                                                                                             >  1:4195668/1‑100 (MQ=255)   ttAGATAAAACCATGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATa                                                                                           >  1:1736579/1‑100 (MQ=255)    tAGATAAAACCATGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATaa                                                                                          >  1:1667945/1‑100 (MQ=255)     aGATAAAACCATGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATaaa                                                                                         >  1:983736/1‑100 (MQ=255)             acATGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTa                                                                                 >  1:3418432/2‑100 (MQ=255)               ttGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTaaa                                                                               >  1:5585442/2‑100 (MQ=255)               aTGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTaaa                                                                               >  1:1491437/1‑100 (MQ=255)               aTGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTaaa                                                                               >  1:814580/1‑100 (MQ=255)               aTGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTCACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTaaa                                                                               >  1:4497433/1‑100 (MQ=255)                tGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAAt                                                                              <  1:212758/100‑1 (MQ=255)                 ggCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTcagatcg                                                                             >  1:3834731/1‑94 (MQ=255)                 ggCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATg                                                                             >  1:866779/1‑100 (MQ=255)                 ggCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATg                                                                             >  1:3795357/1‑100 (MQ=255)                 ggCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATg                                                                             >  1:1484846/1‑100 (MQ=255)                  gCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATgg                                                                            >  1:2093051/1‑100 (MQ=255)                   ttCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGa                                                                           >  1:2973837/2‑100 (MQ=255)                     cAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGatt                                                                         >  1:5524522/1‑100 (MQ=255)                     cAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGatt                                                                         >  1:4570096/1‑100 (MQ=255)                      aaCTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGatta                                                                        >  1:5630811/1‑100 (MQ=255)                       gtgtgcAAAAGAACAAAAAGGGCAAGTTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGattat                                                                       >  1:2739047/6‑100 (MQ=255)                       aCTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGattat                                                                       >  1:5085810/1‑100 (MQ=255)                          ttCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGattagatc                                                                    >  1:4986848/1‑96 (MQ=255)                          ttCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATtgt                                                                    >  1:683189/1‑100 (MQ=255)                          ttCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATtgt                                                                    >  1:2229143/1‑100 (MQ=255)                          ttCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATtgc                                                                    >  1:70936/1‑99 (MQ=255)                           tCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATtgtg                                                                   <  1:4220969/100‑1 (MQ=255)                           tCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATtgtg                                                                   >  1:1686554/1‑100 (MQ=255)                              gctgcaCAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTgtgagt                                                                >  1:3883152/6‑100 (MQ=255)                              aaaGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTgtgagt                                                                >  1:859483/1‑100 (MQ=255)                               aaGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTgtgagtg                                                               >  1:4435895/1‑100 (MQ=255)                                aGAACAAAACGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTgtgagtga                                                              >  1:4483850/1‑100 (MQ=255)                                aGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTgtgagtga                                                              >  1:2741416/1‑100 (MQ=255)                                aGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTgtgagtga                                                              >  1:2034809/1‑100 (MQ=255)                                aGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTgtgagtga                                                              >  1:1871028/1‑100 (MQ=255)                                 taaCAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGAt                                                             >  1:2356518/2‑100 (MQ=255)                                 gcaCAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGAt                                                             >  1:649980/3‑100 (MQ=255)                                 gAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGAt                                                             >  1:3597090/1‑100 (MQ=255)                                   aCAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATaa                                                           >  1:74528/1‑100 (MQ=255)                                       ctaGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCAt                                                       >  1:3704907/3‑100 (MQ=255)                                       ctaGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTATGAGTGATAACCAt                                                       >  1:3220124/3‑100 (MQ=255)                                        aaGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATa                                                      >  1:3430259/1‑100 (MQ=255)                                         aGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATAc                                                     >  1:5207378/1‑100 (MQ=255)                                         aGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATAc                                                     >  1:2912585/1‑100 (MQ=255)                                          gggCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACt                                                    >  1:1579318/1‑100 (MQ=255)                                           ggCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTg                                                   >  1:3313897/1‑100 (MQ=255)                                           ggCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTg                                                   >  1:3743172/1‑100 (MQ=255)                                           ggCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTg                                                   >  1:4619856/1‑100 (MQ=255)                                                  tAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAgg                                            >  1:2164652/1‑100 (MQ=255)                                                   tgccATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGc                                           >  1:767575/4‑100 (MQ=255)                                                     tCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGt                                         >  1:624223/1‑100 (MQ=255)                                                      cttAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtt                                        >  1:3783837/3‑100 (MQ=255)                                                      cATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtt                                        >  1:678846/1‑100 (MQ=255)                                                      cATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtt                                        >  1:2375813/1‑100 (MQ=255)                                                      cATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtt                                        >  1:2076956/1‑100 (MQ=255)                                                      cATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtt                                        >  1:5619488/1‑100 (MQ=255)                                                      cATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtt                                        >  1:603150/1‑100 (MQ=255)                                                      cATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtt                                        >  1:1534077/1‑100 (MQ=255)                                                      cATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtt                                        >  1:2985858/1‑100 (MQ=255)                                                      cATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtt                                        >  1:1606767/1‑100 (MQ=255)                                                      cATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGCCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtt                                        >  1:4133514/1‑100 (MQ=255)                                                       ctAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGttt                                       >  1:733335/2‑100 (MQ=255)                                                       acaGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGttt                                       >  1:70019/3‑100 (MQ=255)                                                       aTAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGttt                                       >  1:4538570/1‑100 (MQ=255)                                                        tAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtttt                                      >  1:4829405/1‑100 (MQ=255)                                                        ctctagAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGtttt                                      >  1:2004708/6‑100 (MQ=255)                                                         aGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTta                                     >  1:27440/1‑100 (MQ=255)                                                          gACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTtat                                    >  1:3597014/1‑100 (MQ=255)                                                          gACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTtat                                    >  1:4038626/1‑100 (MQ=255)                                                          aaCGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTtat                                    >  1:1923734/2‑100 (MQ=255)                                                           aCGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTtata                                   >  1:3824600/1‑100 (MQ=255)                                                               cctGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCaa                               >  1:1963013/3‑100 (MQ=255)                                                               aaTGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCaa                               >  1:100561/1‑100 (MQ=255)                                                                ctGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCaaa                              >  1:2344335/2‑100 (MQ=255)                                                                ctGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCaaa                              >  1:1155748/2‑100 (MQ=255)                                                                aTGCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCaaa                              >  1:1280622/1‑100 (MQ=255)                                                                  gctaTTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCAAAtt                            >  1:3011749/4‑100 (MQ=255)                                                                  gCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCAAAtt                            >  1:4235219/1‑100 (MQ=255)                                                                  gCGATTAATTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCAAAtt                            >  1:3267030/1‑100 (MQ=255)                                                                        taTTTACTTCAAGCTTTTCTTGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCAAATTTATGaa                      >  1:2423457/2‑100 (MQ=255)                                                                                            tGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCAAATTTATGAAATGCACCcagatcggaagag  >  1:1151287/1‑92 (MQ=255)                                                                                            tGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCAAATTTATGAAATGCACCcagatcggaagag  >  1:2978953/1‑92 (MQ=255)                                                                                            tGAAGATGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCAAATTTATGAAATGCACCCAGATGCAGATGt  >  1:4879262/1‑100 (MQ=255)                                                                                                 |                                                                                               AATTAGATAAAACCATGGCTCAACTTTCAAAAGAACAAAAAGGGCAAATTAGTCATAGACGAAATGCGATTAATTTACTTCAAGCTTTTCTTGAAGGTGATAAAAATGTCTAAATGGATTATTGTGAGTGATAACCATACTGAATCAGGCGTTTTATATCAAATTTATGAAATGCACCCAGATGCAGATGT  >  NC_007793/1149563‑1149753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑