Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,143,651	T→C	S322P (TCT→CCT)	SAUSA300_RS05630 →	excinuclease ABC subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,143,651	0	T	C	100.0%	80.9 / NA	31	S322P (TCT→CCT)	SAUSA300_RS05630	excinuclease ABC subunit C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/1);  total (30/1)

ATACATTTATTGGACAATTTTATAGCTTAAACCAACATATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAATCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAATAATA  >  NC_007793/1143559‑1143747                                                                                             |                                                                                                 atgcATTTATTGGACAATTTTATAGCTTAAACCAACATATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCtgttgt                                                                                           >  1:2802544/4‑100 (MQ=255)           tGGACAATTTTATAGCTTCCCCCAACATATTTGACCTAAGGAAGTTCATGTACCACGTAATGTGGATAAAGAAATGATTCAACCTGTTGTGGACACTaaa                                                                                 >  1:5220919/1‑100 (MQ=255)                     tataGCTTAAACCAACATATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAAc                                                                       >  1:26658/1‑100 (MQ=255)                            tAAACCAACATATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCcgcgcg                                                                <  1:3256103/100‑1 (MQ=255)                                 gaaCATATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGATc                                                           >  1:1640019/2‑100 (MQ=255)                                           tACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGa                                                 >  1:2760418/1‑100 (MQ=255)                                                      tttCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGAcc                                      >  1:988836/2‑100 (MQ=255)                                                           tGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCt                                 >  1:3498025/1‑100 (MQ=255)                                                           agTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCt                                 >  1:4744205/2‑100 (MQ=255)                                                            gTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTg                                >  1:788523/1‑100 (MQ=255)                                                            gTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTg                                >  1:753304/1‑100 (MQ=255)                                                            gTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTg                                >  1:4206285/1‑100 (MQ=255)                                                            gTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTg                                >  1:1796216/1‑100 (MQ=255)                                                            gTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCAAGGTCCCAAAAAAGATATGGTTGACCTAGCTg                                >  1:3670652/1‑100 (MQ=255)                                                                         tGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTaa                   >  1:1429762/1‑100 (MQ=255)                                                                                      gATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAaat      >  1:1013768/1‑100 (MQ=255)                                                                                      gATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAaat      >  1:5049142/1‑100 (MQ=255)                                                                                       tttCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAaata     >  1:2576187/2‑100 (MQ=255)                                                                                        ctCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAaataa    >  1:3820424/2‑100 (MQ=255)                                                                                        atCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAaataa    >  1:1470785/2‑100 (MQ=255)                                                                                         tCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAaataat   >  1:3922661/1‑100 (MQ=255)                                                                                         gcAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAaataat   >  1:3675299/2‑100 (MQ=255)                                                                                          cAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACCTAACGCTAAAGTATCCTTAAataata  >  1:1696601/1‑100 (MQ=255)                                                                                          cAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAataata  >  1:4025352/1‑100 (MQ=255)                                                                                          cAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAataata  >  1:4046007/1‑100 (MQ=255)                                                                                          cAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAataata  >  1:168989/1‑100 (MQ=255)                                                                                          cAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAataata  >  1:106053/1‑100 (MQ=255)                                                                                          cAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAataata  >  1:5377178/1‑100 (MQ=255)                                                                                          cAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAataata  >  1:2249399/1‑100 (MQ=255)                                                                                          cAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAataata  >  1:2204580/1‑100 (MQ=255)                                                                                          cAACCTGTTGTGGACACTAAAATCGCTCAACCCCCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGCATCCTTCAataata  >  1:2262874/1‑100 (MQ=255)                                                                                             |                                                                                                 ATACATTTATTGGACAATTTTATAGCTTAAACCAACATATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAATCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAATAATA  >  NC_007793/1143559‑1143747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑