Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,122,225	G→A	intergenic (+28/+126)	rpmF → / ← SAUSA300_RS05535	50S ribosomal protein L32/iron‑regulated surface determinant protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,122,225	0	G	A	84.6%	19.4 / ‑2.1	13	intergenic (+28/+126)	rpmF/SAUSA300_RS05535	50S ribosomal protein L32/iron‑regulated surface determinant protein B
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (0/11);  total (0/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.68e-01

GAATACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCGCTTAAAATGATT  >  NC_007793/1122126‑1122237                                                                                                    |             gAATACAAATTATCGCACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCg              <  1:3673915/100‑1 (MQ=255)   aTACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACt            <  1:4829003/100‑1 (MQ=255)    tACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTGCAATGGCGAATAAGTAGCAGCTAAATAATTTTAGTTACTCAATAGAAAAAGGCCCACtg           <  1:5459813/100‑2 (MQ=255)    tACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCGCtt           <  1:718715/100‑1 (MQ=255)    tACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACtt           <  1:1433480/100‑1 (MQ=255)    tACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACtt           <  1:1632336/100‑1 (MQ=255)    tACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACtt           <  1:3389162/100‑1 (MQ=255)    tACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACtt           <  1:5264678/100‑1 (MQ=255)    tACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACtt           <  1:5515650/100‑1 (MQ=255)    tACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACtt           <  1:653865/100‑1 (MQ=255)     aCAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAGGTCCCACTTa          <  1:4242168/100‑1 (MQ=255)     aCAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACTTa          <  1:1018459/100‑1 (MQ=255)        aaTTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCGCTTaaaa       <  1:5108370/100‑1 (MQ=255)             tCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACTTaaaacaaag  <  1:5528071/100‑6 (MQ=255)                                                                                                    |             GAATACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCGCTTAAAATGATT  >  NC_007793/1122126‑1122237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑