Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,116,591	T→G	G330G (GGT→GGG)	SAUSA300_RS05480 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,116,591	0	T	G	100.0%	194.6 / NA	72	G330G (GGT→GGG)	SAUSA300_RS05480	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (41/31);  total (41/31)

GTTATGCGTTTAATGATGTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGTGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATATTAGAT  >  NC_007793/1116494‑1116687                                                                                                  |                                                                                                 gTTATGCGTTTAATGATGTGCCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATgggggg                                                                                                <  1:976880/100‑1 (MQ=255)             atgatgTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATgag                                                                                    <  1:3303379/100‑1 (MQ=255)               cctgTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATgagag                                                                                  >  1:3261710/3‑100 (MQ=255)                  gTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGatt                                                                               >  1:3649567/1‑100 (MQ=255)                    aCCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGattat                                                                             >  1:3962932/1‑100 (MQ=255)                             aaTCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTa                                                                    >  1:3355353/1‑100 (MQ=255)                                ccATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAAtt                                                                 >  1:318319/1‑100 (MQ=255)                                ccATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGCAATGAGAGATTATTATTCACTAAtt                                                                 >  1:1847328/1‑100 (MQ=255)                                 gctAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAAttt                                                                >  1:3969531/3‑100 (MQ=255)                                   tAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTc                                                              >  1:5704440/1‑100 (MQ=255)                                                tCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAAc                                                 >  1:1382216/1‑100 (MQ=255)                                                      ctcttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAg                                           <  1:1356967/94‑1 (MQ=255)                                                      ctcttccGATCTCGACTGTTAAATTCAAAATACGATAAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAg                                           <  1:4646463/94‑1 (MQ=255)                                                       tcttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgg                                          <  1:4325070/95‑1 (MQ=255)                                                       tcttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgg                                          <  1:5089755/95‑1 (MQ=255)                                                       tcttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgg                                          <  1:808584/95‑1 (MQ=255)                                                       tcttccGATCTCGTC‑ATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggg                                         <  1:516496/95‑1 (MQ=255)                                                       tcttccGATCTCGGCTATTAAATTCAAAATACGATGGGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgg                                          <  1:3718072/95‑1 (MQ=255)                                                       taCATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgg                                          >  1:208150/1‑100 (MQ=255)                                                        cttccGATCTGGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggg                                         <  1:2173424/96‑1 (MQ=255)                                                        cttccGATCTCGTCTGTTAAATTCAAAATTCGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgga                                         <  1:3476231/96‑2 (MQ=255)                                                        cttccGATCTCGTCTGTTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggg                                         <  1:2220682/96‑1 (MQ=255)                                                        cttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggg                                         <  1:391157/96‑1 (MQ=255)                                                        cttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggg                                         <  1:292540/96‑1 (MQ=255)                                                        cttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggg                                         <  1:192012/96‑1 (MQ=255)                                                         ttccGATCTCGACTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgggg                                        <  1:3605733/97‑1 (MQ=255)                                                          tccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggggg                                       <  1:4632928/98‑2 (MQ=255)                                                          tccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggggc                                       <  1:2989796/98‑2 (MQ=255)                                                          tccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggggc                                       <  1:3145323/98‑2 (MQ=255)                                                          tccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGt                                       <  1:5643002/98‑1 (MQ=255)                                                          tccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAAGGGt                                       <  1:3002679/98‑1 (MQ=255)                                                           ccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTa                                      <  1:2385626/99‑1 (MQ=255)                                                            cGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGTGTaa                                     <  1:5415464/100‑2 (MQ=255)                                                             gATCTTGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTAtt                                    <  1:4095651/100‑1 (MQ=255)                                                             gATCTCGTCTGTTAAATTCAAAATATGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggggcaaa                                    <  1:478795/100‑5 (MQ=255)                                                             gATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTAtt                                    <  1:2397150/100‑1 (MQ=255)                                                             gATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTACTCACTAATTTTCTTAGAAAAATAACAATGAggggcaag                                    <  1:3296360/100‑5 (MQ=255)                                                                ctcGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggggctagatc                                 >  1:3419044/1‑93 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGTTGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggggagatcggaa                               >  1:5101648/1‑91 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgggtagatcggaa                               >  1:3196964/1‑90 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggggcagatcgga                               >  1:4933707/1‑91 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggggatagatcgg                               >  1:5743914/1‑91 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgggagatcggaag                               >  1:1669174/1‑90 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTagatcgga                               >  1:2471599/1‑93 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGa                               >  1:1282572/1‑100 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGa                               >  1:1282547/1‑100 (MQ=255)                                                                                  aaTACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACt               <  1:1635839/100‑1 (MQ=255)                                                                                  aaTACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACt               >  1:4207922/1‑100 (MQ=255)                                                                                     aCGATGAGATGGGGGGAGATGTAATGAGGGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGc            >  1:3090721/1‑100 (MQ=255)                                                                                     aCGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCagatcg            >  1:4879069/1‑95 (MQ=255)                                                                                     aCGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGc            >  1:1745157/1‑100 (MQ=255)                                                                                     aCGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGc            >  1:2653521/1‑100 (MQ=255)                                                                                     aCGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGc            >  1:3007465/1‑100 (MQ=255)                                                                                      acctGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCg           >  1:1536665/4‑100 (MQ=255)                                                                                        gtGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGat         >  1:1777695/2‑100 (MQ=255)                                                                                         tGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGata        >  1:4076422/1‑100 (MQ=255)                                                                                          tagaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGatat       >  1:2055499/2‑100 (MQ=255)                                                                                          gagaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGatat       >  1:1338098/1‑100 (MQ=255)                                                                                          gagaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGatat       >  1:747108/1‑100 (MQ=255)                                                                                          gagaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGatat       >  1:1401821/1‑100 (MQ=255)                                                                                          gagaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGatat       >  1:5043330/1‑100 (MQ=255)                                                                                          gagaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGatat       >  1:1814461/1‑100 (MQ=255)                                                                                          gagaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGatat       >  1:5350866/1‑100 (MQ=255)                                                                                           gcttGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTGATGAAGCTTCACTAGCGATAtt      >  1:4897025/4‑100 (MQ=255)                                                                                           gactGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATAtt      >  1:2864599/4‑100 (MQ=255)                                                                                           agaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATAtt      <  1:922191/100‑1 (MQ=255)                                                                                           agaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATAtt      >  1:327921/1‑100 (MQ=255)                                                                                           agaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATAtt      <  1:5638303/100‑1 (MQ=255)                                                                                           agaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATAtt      <  1:1965292/100‑1 (MQ=255)                                                                                           agaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATAtt      <  1:2857427/100‑1 (MQ=255)                                                                                             gtGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGGAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATATTAg    >  1:1513488/2‑100 (MQ=255)                                                                                             aTGGGGGGAAATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATATTAg    >  1:3719020/1‑100 (MQ=255)                                                                                              tGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATATTAga   >  1:764485/1‑100 (MQ=255)                                                                                               ggggggAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATATTAgat  >  1:2363787/1‑100 (MQ=255)                                                                                               ggggggAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATATTAgat  >  1:2441179/1‑100 (MQ=255)                                                                                               ggggggAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAAGGAGGGGTATTTATGATTAATGAAGCTTCACTATCGATATTAgag  >  1:261312/1‑99 (MQ=255)                                                                                                  |                                                                                                 GTTATGCGTTTAATGATGTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGTGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATATTAGAT  >  NC_007793/1116494‑1116687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑