Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,113,988	G→A	A37A (GCG→GCA)	SAUSA300_RS05465 →	protoheme IX farnesyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,113,988	0	G	A	100.0%	163.8 / NA	58	A37A (GCG→GCA)	SAUSA300_RS05465	protoheme IX farnesyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (58/0);  total (58/0)

ACTTTGTCACAAAATATTAGCAGAGTTAACTTCAAAGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCGGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATGGGT  >  NC_007793/1113893‑1114084                                                                                                |                                                                                                 aCTTTGTCACAAAATATTAGCAGAGTTAACTTCAAAGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGgcg                                                                                              >  1:1363427/1‑100 (MQ=255) aCTTTGTCACAAAATATTAGCAGAGTTAACTTCAAAGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGgcg                                                                                              >  1:4029269/1‑100 (MQ=255)     tGTCACAAAATATTAGCAGAGTTAACTTCAAGGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATg                                                                                          >  1:5365777/1‑100 (MQ=255)      atCACAAAATATTAGCAGAGTTAACTTCAAAGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGgcagatcg                                                                                         >  1:4788895/2‑94 (MQ=255)                atTAGCAGAGTTAACTTCAAAGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTg                                                                               >  1:4041474/1‑100 (MQ=255)                 ttAGCAGAGTTAACTTCAAAGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGt                                                                              >  1:4999987/1‑100 (MQ=255)                  tAGCAGAGTTAACTTCAAAGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTa                                                                             >  1:1326778/1‑100 (MQ=255)                  tAGCAGAGTTAACTTCAAAGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTa                                                                             >  1:2781842/1‑100 (MQ=255)                   aGCAGAGTTAACTTCAAAGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAgatcgga                                                                            >  1:167227/1‑94 (MQ=255)                   aGCAGAGTTAACTTCAAAGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTaa                                                                            >  1:3761800/1‑100 (MQ=255)                                        ttGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTat                                                       >  1:170673/1‑100 (MQ=255)                                         tGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTatc                                                      >  1:1997260/1‑100 (MQ=255)                                         tGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTatc                                                      >  1:2494222/1‑100 (MQ=255)                                           cAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTatcat                                                    >  1:767803/1‑100 (MQ=255)                                            aaCAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTatcatc                                                   >  1:4754781/1‑100 (MQ=255)                                             aCAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTAtcatca                                                  >  1:991357/1‑100 (MQ=255)                                              cAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGCCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCaa                                                 >  1:5527753/1‑100 (MQ=255)                                               aGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAAt                                                >  1:5514307/1‑100 (MQ=255)                                                      gacctggggACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCaa                                         >  1:4423558/7‑100 (MQ=255)                                                       aaaaTGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCaaa                                        >  1:347589/1‑100 (MQ=255)                                                          aTGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGTTCATGGCTAGCAGATGTAATTACAAATCATTCCTTCTTATCATCAATACCTCAAAttt                                     >  1:3566306/1‑100 (MQ=255)                                                           agggACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAAtttt                                    >  1:907435/2‑100 (MQ=255)                                                            gggACTTGTTCAAGGTAACTTACTTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTtta                                   >  1:662983/1‑100 (MQ=255)                                                            gggACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTtta                                   >  1:3367784/1‑100 (MQ=255)                                                                tgtGTTCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTaa                               >  1:869571/3‑100 (MQ=255)                                                                     tCAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTa                          >  1:1423551/1‑100 (MQ=255)                                                                     ccAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTa                          >  1:2105973/2‑100 (MQ=255)                                                                      cAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCtct                         >  1:4037674/1‑98 (MQ=255)                                                                      cAAGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTAt                         >  1:5686559/1‑100 (MQ=255)                                                                       aaGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTAtt                        >  1:3510429/1‑100 (MQ=255)                                                                       aaGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTAtt                        >  1:2007775/1‑100 (MQ=255)                                                                       aaGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTAtt                        >  1:760722/1‑100 (MQ=255)                                                                        aGGTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTAttt                       >  1:953090/1‑100 (MQ=255)                                                                          gTAACTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTgg                     >  1:3321272/1‑100 (MQ=255)                                                                            aaCTTAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGATAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGAt                   >  1:879384/1‑100 (MQ=255)                                                                                tAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTAc               >  1:3545724/1‑100 (MQ=255)                                                                                tAATTCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTAc               >  1:2719294/1‑100 (MQ=255)                                                                                   ttCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACttt            >  1:2561658/1‑100 (MQ=255)                                                                                   cctcGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACttt            >  1:5212391/4‑100 (MQ=255)                                                                                    tCCGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTa           >  1:1275836/1‑100 (MQ=255)                                                                                     ctggCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTaa          >  1:366475/3‑100 (MQ=255)                                                                                     ccGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTaa          >  1:1380119/1‑100 (MQ=255)                                                                                     ccGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTaa          >  1:3565770/1‑100 (MQ=255)                                                                                     ccGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTaa          >  1:2423048/1‑100 (MQ=255)                                                                                     acGGCTTTTGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTaa          >  1:2152244/2‑100 (MQ=255)                                                                                       ggCTTTTGCAGGCACATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCGTCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAAtt        >  1:1069494/1‑100 (MQ=255)                                                                                           tttGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATgg    >  1:3954815/1‑100 (MQ=255)                                                                                           tttGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATgg    >  1:134403/1‑100 (MQ=255)                                                                                           tttGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATgg    >  1:1503902/1‑100 (MQ=255)                                                                                           tttGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATgg    >  1:2411281/1‑100 (MQ=255)                                                                                           tttGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCCTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATgg    >  1:5449929/1‑100 (MQ=255)                                                                                            ttGCAGGCGCATGGCTAGCAGTTGTACTGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATggg   >  1:5223965/1‑100 (MQ=255)                                                                                            ttGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATggg   >  1:4576486/1‑100 (MQ=255)                                                                                            ttGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATggg   >  1:3648872/1‑100 (MQ=255)                                                                                            ttGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATggg   >  1:3690508/1‑100 (MQ=255)                                                                                            ttGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATggg   >  1:4622862/1‑100 (MQ=255)                                                                                            atGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATggg   >  1:2705628/2‑100 (MQ=255)                                                                                             tGCAGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATGGGt  >  1:61508/1‑100 (MQ=255)                                                                                                |                                                                                                 ACTTTGTCACAAAATATTAGCAGAGTTAACTTCAAAGAATTGCAACAGATAATTAAAATGGGACTTGTTCAAGGTAACTTAATTCCGGCTTTTGCGGGCGCATGGCTAGCAGTTGTAATGACAAATCATTCCTTCTTATCATCAATACCTCAAATTTTATTAATGCTATTTGGATCTACTTTAATTATGGGT  >  NC_007793/1113893‑1114084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑