Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,112,363	C→T	I1143I (ATC→ATT)	SAUSA300_RS05455 →	pyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,112,363	0	C	T	100.0%	80.8 / NA	29	I1143I (ATC→ATT)	SAUSA300_RS05455	pyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/6);  total (23/6)

GGAAACAACAATTCAAGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATCGAAATTGAAAAAGCAACTGACTAAAATGATTAAAATAAAACGAGATTACACAACAATTGACGTGTAATCTCGTTTTTACATGGCTTAGG  >  NC_007793/1112273‑1112452                                                                                           |                                                                                          ggAAACAACAATTCAAGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAattgaaattgaa                                                                                  >  1:3824833/1‑100 (MQ=255) ggAAACAACAATTCAAGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAattgaaattgaa                                                                                  >  1:243777/1‑100 (MQ=255)   taacaacAATTCAAGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGaaaa                                                                                >  1:3178000/2‑100 (MQ=255)   aaaCAACAATTCAAGCACCATTTGACGGTGTGATTAAACAAGCAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGaaaa                                                                                >  1:3662913/1‑100 (MQ=255)         caaTTCAAGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAAc                                                                          <  1:3530871/100‑1 (MQ=255)            ttCAAGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGTAACTGa                                                                       >  1:3046750/1‑100 (MQ=255)            ttCAAGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGa                                                                       <  1:5717385/100‑1 (MQ=255)              cAAGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAAcaagat                                                                     >  1:3037025/1‑95 (MQ=255)               caGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAAccaacag                                                                    >  1:2808930/2‑94 (MQ=255)                aGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTaa                                                                   >  1:2314977/1‑100 (MQ=255)                    ccATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAGAtc                                                               >  1:1309965/1‑99 (MQ=255)                      ttttGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGAt                                                             >  1:5285433/2‑100 (MQ=255)                        ttGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTagatcggaag                                                           >  1:1146878/1‑91 (MQ=255)                        ttGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAGAtcggaa                                                           >  1:1360617/1‑95 (MQ=255)                        ttGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATta                                                           >  1:3031872/1‑100 (MQ=255)                        ttGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATta                                                           >  1:876342/1‑100 (MQ=255)                        ttGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATta                                                           >  1:4596883/1‑100 (MQ=255)                         taccGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATtaa                                                          >  1:1331534/4‑100 (MQ=255)                         tGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGATAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATtaa                                                          <  1:2000819/100‑1 (MQ=255)                         tGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATtaa                                                          >  1:4148616/1‑100 (MQ=255)                         tGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATtaa                                                          >  1:4373321/1‑100 (MQ=255)                         tGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATtaa                                                          >  1:5766303/1‑100 (MQ=255)                         tGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATtaa                                                          >  1:676853/1‑100 (MQ=255)                         tGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATtaa                                                          >  1:814337/1‑100 (MQ=255)                          gACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATtaaa                                                         >  1:1600572/1‑100 (MQ=255)                          gACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATtaaa                                                         >  1:1046447/1‑100 (MQ=255)                                gtgATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATtaaaataaaa                                                   >  1:306233/1‑100 (MQ=255)                                         cAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATTAAAATAAAACGAGATTag                                          <  1:4214815/100‑2 (MQ=255)                                           aGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATTAAAATAACACGAGAtcggaa                                        >  1:4069861/1‑95 (MQ=255)                                                                              gCGATTTATTAATTGAAATTGAAAATGCAACTGCCTAAAATGATTAAATTAAAACGAGATTACACAACAATTGACGTGTAATCTCTTTTTTACATGGCtt     <  1:4355330/100‑1 (MQ=255)                                                                                 aTTTATTAATTGAAATTGAAAAAGCAACTGACTAAAATGATTAAAATAAAACGAGATTACACAACAATTGACGTGTAATCTCGTTTTTACATGGCTTAgg  <  1:3688437/100‑1 (MQ=255)                                                                                           |                                                                                          GGAAACAACAATTCAAGCACCATTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGATTTATTAATCGAAATTGAAAAAGCAACTGACTAAAATGATTAAAATAAAACGAGATTACACAACAATTGACGTGTAATCTCGTTTTTACATGGCTTAGG  >  NC_007793/1112273‑1112452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑