Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,045,185	G→A	T935T (ACC→ACT)	SAUSA300_RS05135 ←	bifunctional autolysin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,045,185	0	G	A	100.0%	54.1 / NA	20	T935T (ACC→ACT)	SAUSA300_RS05135	bifunctional autolysin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/17);  total (3/17)

AATTTGGTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCGGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAGATACTTGTTTCGT  >  NC_007793/1045094‑1045280                                                                                            |                                                                                                atcttGGTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGc                                                                                         <  1:4216033/97‑1 (MQ=255) aaTTTGGTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTgt                                                                                         <  1:223706/100‑2 (MQ=255) aaTTTGGTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGc                                                                                         <  1:537046/100‑1 (MQ=255) aaTTTGGTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGc                                                                                         <  1:5221699/100‑1 (MQ=255) aaTTTGGTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGc                                                                                         <  1:4812271/100‑1 (MQ=255)      ggTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTa                                                                                    <  1:3539309/100‑1 (MQ=255)                       accattaccattTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTAcc                                                                   >  1:4078026/1‑100 (MQ=255)                            taccattTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAAcc                                                              <  1:3337163/100‑1 (MQ=255)                                 tttttAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTgg                                                         <  1:872831/100‑2 (MQ=255)                                        ttACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGttatc                                                  <  1:1249365/100‑2 (MQ=255)                                           cATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAAt                                               <  1:5272381/100‑1 (MQ=255)                                             tAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGtgtgtgctat                                             <  1:1191166/100‑10 (MQ=255)                                                  gtAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCg                                        <  1:2101101/100‑1 (MQ=255)                                                               ttGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACaa                           <  1:5335710/100‑2 (MQ=255)                                                               ttGGCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACAt                           >  1:4456265/1‑100 (MQ=255)                                                                 ggCAGCTGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCt                         >  1:326298/1‑100 (MQ=255)                                                                       tGATGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCt                   <  1:5510250/100‑1 (MQ=255)                                                                         aTGTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTAc                 <  1:2922561/100‑1 (MQ=255)                                                                           gTAGTTGGTTTCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAg               <  1:4036993/100‑1 (MQ=255)                                                                                     tCACAGCAGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAGATACTTGttt     <  1:3961485/100‑1 (MQ=255)                                                                                        cagcagTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAGATACTTGTTTCGt  <  1:2048442/100‑1 (MQ=255)                                                                                        cagcagTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAGATACTTGTTTCGt  <  1:627085/100‑1 (MQ=255)                                                                                            |                                                                                                AATTTGGTGTTACATAGTAATAACCATTACCATTTTTAATTACATAAGTGTAGTTATAATCTTTGGCAGCTGATGTAGTTGGTTTCACAGCGGTTGGTGCAGTTAAATCTTTTGCATTTACCCAACCAGTGCGGTTATTAATAGTACCGTATAAATAAACATCTTTGCCTACAGATACTTGTTTCGT  >  NC_007793/1045094‑1045280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑