D66 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,033,580	G→A	G178E (GGA→GAA)	SAUSA300_RS05080 →	isochorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,033,580	0	G	A	99.6%	827.2 / ‑6.2	271	G178E (GGA→GAA)	SAUSA300_RS05080	isochorismate synthase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (268/1); total (270/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 286 total aligned reads displayed.

ACAGTTAAAAGGGAAAGTTTTACTGTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGGAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTAGC  >  NC_007793/1033483‑1033676

  aGTTAAAAGGGAAAGTTTTACTGTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGAAAtt                                                                                              >  1:3344835/1‑100 (MQ=255)

     gtcaggggAAAGTTTTACTGTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGGaaattac                                                                                           >  1:3641718/6‑95 (MQ=255)

      aaaaGGGAAAGTTTTACTGTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTa                                                                                          >  1:449654/1‑100 (MQ=255)

       caaGGGAAAGTTTTACTGTTGAGGCATTGTACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGATGAGCAAATTGGGGAAATTACTAg                                                                                         >  1:3685107/2‑100 (MQ=255)

                    tACTGTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATAtt                                                                            >  1:2263918/1‑100 (MQ=255)

                        gTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTtata                                                                        >  1:1540703/1‑100 (MQ=255)

                         ctGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATaa                                                                       >  1:1748691/2‑100 (MQ=255)

                           gAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAg                                                                     >  1:3866454/1‑100 (MQ=255)

                           aatgCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAg                                                                     >  1:1610804/4‑100 (MQ=255)

                            tggCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGa                                                                    >  1:5460285/2‑100 (MQ=255)

                                                        caacaaTATATCGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCt                                        >  1:325343/1‑100 (MQ=255)

                                                              catatCGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGaa                                  >  1:2970985/2‑100 (MQ=255)

                                                                 ctCGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCt                               >  1:1136699/2‑100 (MQ=255)

                                                                  cgggACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTa                              >  1:5305980/3‑100 (MQ=255)

                                                                   cGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTAt                             >  1:5633186/1‑100 (MQ=255)

                                                                   cGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTAt                             >  1:5794488/1‑100 (MQ=255)

                                                                   cGGACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTAt                             >  1:1997965/1‑100 (MQ=255)

                                                                   aggACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTAt                             >  1:1499208/2‑100 (MQ=255)

                                                                   aggACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTAt                             >  1:2229915/2‑100 (MQ=255)

                                                                    ggACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTAtt                            >  1:4699745/1‑100 (MQ=255)

                                                                    ggACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTAtt                            >  1:3076031/1‑100 (MQ=255)

                                                                    ggACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTAtt                            >  1:2098153/1‑100 (MQ=255)

                                                                     gACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATta                           >  1:613723/1‑100 (MQ=255)

                                                                     gACATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATta                           >  1:1476223/1‑100 (MQ=255)

                                                                        aTAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATtaata                        >  1:244408/1‑100 (MQ=255)

                                                                        aTAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATtaata                        >  1:5552906/1‑100 (MQ=255)

                                                                         tAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATtaataa                       >  1:267805/1‑100 (MQ=255)

                                                                         tAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATtaataa                       >  1:3759431/1‑100 (MQ=255)

                                                                          aGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTaataat                      >  1:1378749/1‑100 (MQ=255)

                                                                            ttgtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATga                    >  1:2315585/2‑100 (MQ=255)

                                                                            ttgtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATga                    >  1:1302272/2‑100 (MQ=255)

                                                                            ctgtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAGCTTGTAGTAGAAGCTATAGAATCTATTAATAATga                    >  1:2483459/2‑100 (MQ=255)

                                                                            ctgtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATga                    >  1:3832050/2‑100 (MQ=255)

                                                                            ctgtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATga                    >  1:5386306/2‑100 (MQ=255)

                                                                            aTGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATga                    >  1:883168/1‑100 (MQ=255)

                                                                            aTGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATga                    >  1:354442/1‑100 (MQ=255)

                                                                             tgtgGACGCGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTCGTAGAAGCTATAGAATCTATTAATAATgaa                   >  1:4122357/1‑100 (MQ=255)

                                                                             tgtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaa                   >  1:1336497/1‑100 (MQ=255)

                                                                             tgtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaa                   >  1:2857558/1‑100 (MQ=255)

                                                                             tgtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaa                   >  1:5224846/1‑100 (MQ=255)

                                                                             tgtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaa                   >  1:4141233/1‑100 (MQ=255)

                                                                             tgtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaa                   >  1:1719854/1‑100 (MQ=255)

                                                                             tgtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaa                   >  1:4198600/1‑100 (MQ=255)

                                                                             gatgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaa                   >  1:518002/3‑100 (MQ=255)

                                                                              ttgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:2024889/2‑100 (MQ=255)

                                                                              ttgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:4950090/2‑100 (MQ=255)

                                                                              gtgGACGAGCAAATTGGGGAAATTACTTGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:100177/1‑100 (MQ=255)

                                                                              gtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:1694779/1‑100 (MQ=255)

                                                                              gtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:3273490/1‑100 (MQ=255)

                                                                              gtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:4524124/1‑100 (MQ=255)

                                                                              gtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:275723/1‑100 (MQ=255)

                                                                              gtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:3445682/1‑100 (MQ=255)

                                                                              gtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:1255350/1‑100 (MQ=255)

                                                                              gtgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGAGGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:5713878/1‑100 (MQ=255)

                                                                              ctgGACGGGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:2030151/2‑100 (MQ=255)

                                                                              atgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:5132359/2‑100 (MQ=255)

                                                                              atgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaag                  >  1:1926533/2‑100 (MQ=255)

                                                                               tgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaaga                 >  1:2072611/1‑100 (MQ=255)

                                                                               tgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaaga                 >  1:5062445/1‑100 (MQ=255)

                                                                               tgGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaaga                 >  1:4712159/1‑100 (MQ=255)

                                                                                ggACGAGCAAATTGGGGAAATTACTAGAAATGAAGATTTTTATAAAGATGACTGGTGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaagaa                >  1:5580908/1‑100 (MQ=255)

                                                                                ggACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaagaa                >  1:4999759/1‑100 (MQ=255)

                                                                                ggACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaagaa                >  1:585869/1‑100 (MQ=255)

                                                                                ggACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaagaa                >  1:3400434/1‑100 (MQ=255)

                                                                                ggACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaagaa                >  1:1059835/1‑100 (MQ=255)

                                                                                ggACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATgaagaa                >  1:3193922/1‑100 (MQ=255)

                                                                                 ttgctgCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGaaa               >  1:2738213/6‑100 (MQ=255)

                                                                                   ctggCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGaaaaa             >  1:2661223/4‑100 (MQ=255)

                                                                                    tggCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGaaaaaa            >  1:3147423/3‑100 (MQ=255)

                                                                                    gAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGaaaaaa            >  1:3810081/1‑100 (MQ=255)

                                                                                    ccgCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGaaaaaa            >  1:789910/3‑100 (MQ=255)

                                                                                    caGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGaaaaaa            >  1:2508024/2‑100 (MQ=255)

                                                                                    aaGCAAATTGGGGAACTTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGaaaaaa            >  1:4813450/2‑100 (MQ=255)

                                                                                     tgCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAAt           >  1:2532346/2‑100 (MQ=255)

                                                                                     tgCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAAt           >  1:487974/2‑100 (MQ=255)

                                                                                     aGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGGAAAAAt           >  1:5746504/1‑100 (MQ=255)

                                                                                     aGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAAt           >  1:1044727/1‑100 (MQ=255)

                                                                                     aGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAAt           >  1:4379074/1‑100 (MQ=255)

                                                                                      gCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAAtt          >  1:4733854/1‑100 (MQ=255)

                                                                                      gCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAAtt          >  1:5524898/1‑100 (MQ=255)

                                                                                       ctaaTTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTg         >  1:462076/3‑100 (MQ=255)

                                                                                       cAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTg         >  1:2582602/1‑100 (MQ=255)

                                                                                         aaTTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAACATTGTa       >  1:5741960/1‑100 (MQ=255)

                                                                                         aaTTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTa       >  1:189715/1‑100 (MQ=255)

                                                                                         aaTTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTa       >  1:2153119/1‑100 (MQ=255)

                                                                                         aaTTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTa       >  1:5079318/1‑100 (MQ=255)

                                                                                          tctGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCGACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTAc      >  1:5439472/3‑100 (MQ=255)

                                                                                          aTTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTAc      >  1:24063/1‑100 (MQ=255)

                                                                                          aTTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTAc      >  1:143078/1‑100 (MQ=255)

                                                                                           ctGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACt     >  1:4273316/2‑100 (MQ=255)

                                                                                           ctGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACt     >  1:3951564/2‑100 (MQ=255)

                                                                                            tGGGGAAGTTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTa    >  1:4753588/1‑100 (MQ=255)

                                                                                            tGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTa    >  1:4282228/1‑100 (MQ=255)

                                                                                            tGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTa    >  1:1825205/1‑100 (MQ=255)

                                                                                            tGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTa    >  1:752215/1‑100 (MQ=255)

                                                                                            tGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTa    >  1:3182729/1‑100 (MQ=255)

                                                                                            aggggaAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTa    >  1:4086915/2‑100 (MQ=255)

                                                                                             ggggaAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTaa   >  1:3585502/1‑99 (MQ=255)

                                                                                             ggggaAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTAg   >  1:2356038/1‑100 (MQ=255)

                                                                                             ggggaAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTAg   >  1:4570047/1‑100 (MQ=255)

ACAGTTAAAAGGGAAAGTTTTACTGTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATAGATGTGGACGAGCAAATTGGGGGAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTAGC  >  NC_007793/1033483‑1033676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`