| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 1,032,108 | T→A | L253F (TTA→TTT) | SAUSA300_RS05075 ← | 1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 1,032,108 | 0 | T | A | 98.0% | 137.2 / ‑4.4 | 50 | L253F (TTA→TTT) | SAUSA300_RS05075 | 1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (27/21); total (28/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
CTGGCATCATTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAATAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAGCGATAAAGTACATGATTGCCATAAATGTTAAAG > NC_007793/1032017‑1032190 | cTGGCATCATTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGcc < 1:3540938/100‑1 (MQ=255)cTGGCATCATTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGAAAgatcggaagag > 1:2016372/1‑92 (MQ=255) ctgCATCATTGTAGGCGGTGTATCATTCTTCTTGAAACGACTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAA‑AATGAGccac > 1:4053483/3‑100 (MQ=255) aggCATCATTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAAcca > 1:4733111/2‑100 (MQ=255) ggCATCATTGTAGGCGGTGGATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGAAAGGAAaaaaatagatcgg > 1:211430/1‑93 (MQ=255) gCATCATTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGccacc < 1:5106882/100‑1 (MQ=255) gCATCATTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGccacc > 1:2892257/1‑100 (MQ=255) catcatTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACACCGCAAGTAAGTAAACAAATGAAccacca > 1:2466202/1‑100 (MQ=255) catcatTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGccacca > 1:5078615/1‑100 (MQ=255) atcatTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGccaccgc < 1:663751/100‑3 (MQ=255) catTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCATCAggc > 1:559290/1‑99 (MQ=255) catTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAAGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAACGAGCCACCAgga < 1:4651384/100‑2 (MQ=255) ttGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCTCCAGGttt < 1:1222745/100‑1 (MQ=255) ttGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGttt < 1:660797/100‑1 (MQ=255) ttGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGttt < 1:4333858/100‑1 (MQ=255) ccctAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGttt < 1:1904243/97‑1 (MQ=255) tGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTta > 1:3160442/1‑100 (MQ=255) tGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGACCCACCAgatcgg > 1:2063479/1‑95 (MQ=255) tAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCGCCAGGTTtaat < 1:36460/100‑1 (MQ=255) ggCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTtaataa < 1:5636007/100‑1 (MQ=255) ggTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAATAATGAGCCACCAGGTTTAATAAT‑Ga < 1:44940/100‑1 (MQ=255) aTCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTa < 1:4574293/100‑1 (MQ=255) tGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAATAGATCGGAAgagcacacg > 1:576739/1‑92 (MQ=255) tGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAATAGATCGGAAgagcacacg > 1:663842/1‑92 (MQ=255) tGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATaaa > 1:5610069/1‑100 (MQ=255) aCGTCTGATAACTTTAACAGACATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCa < 1:1698878/100‑1 (MQ=255) cGTCTGATATCTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAGGGCAt < 1:5097187/100‑1 (MQ=255) cGTCTGATAACTTTAACAGGCATTGGGAATGATAACCACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCAt < 1:3790990/100‑1 (MQ=255) cGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCAt < 1:5647549/100‑1 (MQ=255) gTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATa < 1:2289886/100‑1 (MQ=255) tCTTATAACTTTAACAGGCAGTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATaa < 1:4445129/100‑1 (MQ=255) gATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAGAGGCagatcgg > 1:1375949/1‑94 (MQ=255) gATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAgat > 1:1496432/1‑98 (MQ=255) gATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAgat > 1:3288354/1‑98 (MQ=255) gATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAgat > 1:1317310/1‑98 (MQ=255) gATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAAGCATAAgat > 1:3822672/1‑98 (MQ=255) tAACTTTAGCAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAGGCGaa < 1:1222861/100‑2 (MQ=255) tAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTGCAATAAAGGCATAAGCGAt < 1:2637460/100‑1 (MQ=255) tttAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAgatcggaag > 1:2801097/1‑92 (MQ=255) ttAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAgatcggaaga > 1:995382/1‑91 (MQ=255) ttAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAGCGATAAAGt > 1:1632664/1‑100 (MQ=255) ttAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAAGCATAAgatcggaaga > 1:2664608/1‑91 (MQ=255) tAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCAAAAgatcggaagag > 1:1573121/1‑90 (MQ=255) aaCAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAGCGATAAAGTAc > 1:5190831/1‑100 (MQ=255) ccAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACGATAAAGGCATAAGCGATAAAGTACa > 1:2271784/2‑100 (MQ=255) aGGCATTGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGCTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAGCGATAAAGTACATg > 1:4534467/1‑100 (MQ=255) ttGGGAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAGCGATAAAGTACATGATTGc > 1:5268410/1‑100 (MQ=255) ggAATGATAACAACGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAGCGATAAAGTACATGATTGCCAt < 1:3750740/100‑1 (MQ=255) aacaacGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAGCGATAAAGTACATGATTGCCATAAATGTTa > 1:704323/1‑100 (MQ=255) acaacGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAGCGATAAAGTACATGATTGCCATAAATGTTaa < 1:5010734/100‑1 (MQ=255) taacGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAGCGATAAAGTACATGATTGCCATAAATGTTaaa > 1:4915565/2‑100 (MQ=255) aacGCAAGTAAGTAAAAAAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAGCGATAAAGTACATGATTGCCATAAATGTTAaag > 1:5719247/1‑100 (MQ=255) | CTGGCATCATTGTAGGCGGTGTATCATTCTTCTTGAAACGTCTGATAACTTTAACAGGCATTGGGAATGATAACAACGCAAGTAAGTAAAATAATGAGCCACCAGGTTTAATAAT‑GATCGTAAGTACAATAAAGGCATAAGCGATAAAGTACATGATTGCCATAAATGTTAAAG > NC_007793/1032017‑1032190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |