Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,023,223	G→A	intergenic (+82/‑244)	SAUSA300_RS05015 → / → SAUSA300_RS05020	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,023,223	0	G	A	100.0%	57.4 / NA	22	intergenic (+82/‑244)	SAUSA300_RS05015/SAUSA300_RS05020	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/4);  total (18/4)

TTGGCACTATATTATATTTAACTTTAGCACTTGGATTATCAACAGCAGCTTATGCATCTACAGAATACGCAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGCTATTGGA  >  NC_007793/1023155‑1023318                                                                     |                                                                                                ttGGCCCTATATTATATTTAACTTTAGCACTTGGATTATCAACAGCAGCTTATGCATCTACAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCgg                                                                  >  1:3905311/1‑100 (MQ=255)  tGGCACTATATTATATTTAACTTTAGCACTTGGATTATCAACAGCAGCTTATGCATCTACAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGc                                                                 <  1:3053873/100‑1 (MQ=255)   ggCACTATATTATATTTAACTTTAGCACTTGGATTATCAACAGCAGCTTATGCATCTACAGAATACACAGAAGGAGGCACTTGGAGGCATggagatcgga                                                                >  1:3071106/1‑92 (MQ=255)         atattatattTAACTTTAGCACTTGGATTATCAACAGCAGCTTATGCATCTACAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCagtaagt                                                          >  1:3226412/1‑100 (MQ=255)                         tggCACTTGGATTATCAACAGCAGCTTATGCATCTACAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGtatta                                          >  1:2244418/3‑100 (MQ=255)                              attGGATTATCAACAGCAGCTTATGCATCTACAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATc                                     >  1:5382812/2‑100 (MQ=255)                                       tctgcagtggcTTATGCATCTACAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATa                            <  1:412077/91‑1 (MQ=255)                                           cagcagCTTATGCATCTACAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATaagcg                        <  1:4564078/100‑4 (MQ=255)                                                   tATGCATCTACAGAATACACAGGAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTg                >  1:2045233/1‑100 (MQ=255)                                                     ccgaTCTACAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCAAGGTCATAAAGGACATGGTGCa              <  1:2471631/97‑1 (MQ=255)                                                          ttACAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGc         >  1:4967606/2‑100 (MQ=255)                                                            gcAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGCTa       >  1:4721542/2‑100 (MQ=255)                                                            aCAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGCTa       >  1:741181/1‑100 (MQ=255)                                                            aCAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGCTa       >  1:1250239/1‑100 (MQ=255)                                                            aCAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGCTa       >  1:4231855/1‑100 (MQ=255)                                                            aCAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGCTa       >  1:3192472/1‑100 (MQ=255)                                                            aCAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGCTa       >  1:190264/1‑100 (MQ=255)                                                            aCAGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGCTa       >  1:1593676/1‑100 (MQ=255)                                                              aGAATACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTCTTATTATCATGGTCATAAAGGACATGGTGCAACAGCTAtt     >  1:5539912/1‑100 (MQ=255)                                                                gaTACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACGTGGTGCAACAGCTATTgg   >  1:3138895/2‑100 (MQ=255)                                                                aaTACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGCTATTgg   >  1:4210629/1‑100 (MQ=255)                                                                 aTACACAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGCTATTGGa  >  1:2376359/1‑100 (MQ=255)                                                                     |                                                                                                TTGGCACTATATTATATTTAACTTTAGCACTTGGATTATCAACAGCAGCTTATGCATCTACAGAATACGCAGAAGGAGGCACTTGGAGTCATGGTGTCGGCAGTAAGTATGTTTGGTCGTATTATTATCATGGTCATAAAGGACATGGTGCAACAGCTATTGGA  >  NC_007793/1023155‑1023318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑