Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	967,086	A→G	R223G (AGA→GGA)	SAUSA300_RS04740 →	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	967,086	0	A	G	100.0%	26.0 / NA	12	R223G (AGA→GGA)	SAUSA300_RS04740	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/5);  total (7/5)

GCTCATTGTCATAACGATCTA‑GGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAGAAAGTACGGAATTCACTTTA  >  NC_007793/966996‑967175                                                                                            |                                                                                          gCTCATTGTCATAACGATCTA‑GGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTg                                                                                  >  1:5717783/1‑100 (MQ=255)     aTTGTCATAACGATCTA‑GGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTgagag                                                                              >  1:4337937/1‑100 (MQ=255)            ttccGATCT‑‑GGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGtt                                                                      <  1:4908386/97‑2 (MQ=255)             tccGATCTATGGGTTGGCTACTGCAAATGCATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGt                                                                       <  1:847030/98‑1 (MQ=255)             tccGATCT‑‑GGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTaa                                                                     <  1:2291995/98‑1 (MQ=255)                     a‑GGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCt                                                              >  1:2525596/1‑100 (MQ=255)                     a‑GGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCt                                                              >  1:5748228/1‑100 (MQ=255)                          ttGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCTATTg                                                          <  1:262381/100‑1 (MQ=255)                                              aGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTg                                      >  1:3103686/1‑100 (MQ=255)                                                cTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGAc                                    >  1:52810/1‑100 (MQ=255)                                                                tGCAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAgga                    <  1:4069229/100‑3 (MQ=255)                                                                                  tACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAAAAAGATCGGaagagcacacg  >  1:578116/1‑91 (MQ=255)                                                                                            |                                                                                          GCTCATTGTCATAACGATCTA‑GGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAGAAAGTACGGAATTCACTTTA  >  NC_007793/966996‑967175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑