Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	961,879	C→T	P375L (CCA→CTA)	SAUSA300_RS04725 →	O‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	961,879	0	C	T	100.0%	48.7 / NA	18	P375L (CCA→CTA)	SAUSA300_RS04725	O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/0);  total (18/0)

TGCAGAGCTATCATATCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACA  >  NC_007793/961796‑961968                                                                                    |                                                                                          tGCAGAGCTATCATATCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATg                                                                           >  1:3434415/1‑100 (MQ=255)          aTCATATCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTgta                                                                  >  1:973305/1‑100 (MQ=255)               atCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAAc                                                             >  1:5222360/1‑100 (MQ=255)                      tataGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTa                                                      >  1:5701017/1‑100 (MQ=255)                           aaaCTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTCGTAACCTTGTTAATTcc                                                 >  1:255417/1‑100 (MQ=255)                            aaCTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCa                                                >  1:1885615/1‑100 (MQ=255)                             aCTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCAt                                               >  1:5734630/1‑100 (MQ=255)                             aCTCCATTTAGAAAAGAAGGAATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCAt                                               >  1:5794825/1‑100 (MQ=255)                                ccATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTa                                            >  1:4992090/1‑100 (MQ=255)                                ccATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTa                                            >  1:5629428/1‑100 (MQ=255)                                ccATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTa                                            >  1:5567819/1‑100 (MQ=255)                                ccATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTa                                            >  1:3341546/1‑100 (MQ=255)                                                                ttGGCGGTCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAatat            >  1:4137115/1‑100 (MQ=255)                                                                ttGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAatat            >  1:646692/1‑100 (MQ=255)                                                                 tGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATg           >  1:392324/1‑100 (MQ=255)                                                                 tGGCGACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATg           >  1:1977807/1‑100 (MQ=255)                                                                     gACCTTCTTATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTaa       >  1:3903192/1‑100 (MQ=255)                                                                          tcttATATACTACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACa  >  1:2926956/1‑100 (MQ=255)                                                                                    |                                                                                          TGCAGAGCTATCATATCGCTTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACA  >  NC_007793/961796‑961968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑