Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	952,565	A→C	K194T (AAA→ACA)	SAUSA300_RS04695 →	ATP‑dependent helicase/nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	952,565	0	A	C	100.0%	76.9 / NA	30	K194T (AAA→ACA)	SAUSA300_RS04695	ATP‑dependent helicase/nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/4);  total (26/4)

GAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTAAAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTATTCAACTACT  >  NC_007793/952468‑952634                                                                                                  |                                                                      gAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTacaa                                                                     >  1:792919/1‑100 (MQ=255) gAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTacaa                                                                     <  1:3232914/100‑1 (MQ=255)   atatggggagCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAAt                                                                   >  1:5735454/8‑100 (MQ=255)     tAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAAttg                                                                 >  1:4662293/1‑100 (MQ=255)     tAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAAttg                                                                 >  1:2392688/1‑100 (MQ=255)      aaCAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAAttgg                                                                >  1:833173/1‑100 (MQ=255)         agacgtgtgctCTTCCGATCTAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATACAAATCCTACAAAttggttg                                                             <  1:11862/90‑1 (MQ=255)          gagCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAAttggttgg                                                            <  1:1130846/100‑1 (MQ=255)           agCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGa                                                           >  1:3201889/1‑100 (MQ=255)                 tGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAAtt                                                     >  1:2838142/1‑100 (MQ=255)                     ctCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGTATCAATTGGTg                                                 >  1:580894/2‑100 (MQ=255)                      tCAGCTAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGa                                                >  1:3901237/1‑100 (MQ=255)                      tCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTgatag                                                >  1:1737825/1‑96 (MQ=255)                      tCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGa                                                >  1:5679044/1‑100 (MQ=255)                       cAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTgcc                                               >  1:1534406/1‑98 (MQ=255)                       cAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGac                                               >  1:5222859/1‑100 (MQ=255)                       cAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGac                                               >  1:4799260/1‑100 (MQ=255)                       cAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGac                                               >  1:930840/1‑100 (MQ=255)                       cAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGac                                               >  1:3706919/1‑100 (MQ=255)                       cAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGac                                               >  1:3628783/1‑100 (MQ=255)                       cAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGac                                               >  1:216958/1‑100 (MQ=255)                       cAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGac                                               >  1:1791986/1‑100 (MQ=255)                       cAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTGGCAAATCCAAATCCTACAAATTGGTTAGATcggaaagcaca                                               >  1:206659/1‑90 (MQ=255)                       cAGATAGAAGTGATGATCAGTTTCGAATGA‑TATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGaca                                              >  1:3528838/1‑100 (MQ=255)                          aTAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACAcc                                            >  1:2126765/1‑100 (MQ=255)                              ttgTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCAtca                                        >  1:4055879/3‑98 (MQ=255)                              aaGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCagat                                        >  1:1379023/1‑97 (MQ=255)                               aGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACTATACg                                       >  1:3114960/1‑100 (MQ=255)                                       ccAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACGCCATAAgatcggaag                               >  1:3486734/2‑93 (MQ=255)                                                   gATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAgcg                   >  1:346158/1‑100 (MQ=255)                                                                    aTTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACATCAAGCGCAACTTATTCAactact  <  1:537838/100‑1 (MQ=255)                                                                    aTTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTATTCAactact  <  1:1625768/100‑1 (MQ=255)                                                                                                  |                                                                      GAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTAAAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTATTCAACTACT  >  NC_007793/952468‑952634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑