Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	944,692	A→T	intergenic (‑46/‑305)	SAUSA300_RS04665 ← / → SAUSA300_RS04670	argininosuccinate synthase/glucose‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	944,692	0	A	T	100.0%	18.0 / NA	8	intergenic (‑46/‑305)	SAUSA300_RS04665/SAUSA300_RS04670	argininosuccinate synthase/glucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (4/4);  total (4/4)

CACACTTGTATCTAGTCCTCCTGAATATGCTAAAACAATTTTCTCTTTCATAAAATTCACCTCATTGTAGTTATCTGAATATTTCGTATGAACACAAGATAGCAAACTTTTAATGAAAATAAAACCATTATTTTACATATTTATTCATAAAATGCGATAGAGATAGTTAGAGTTGT  >  NC_007793/944596‑944771                                                                                                 |                                                                                cacaCTTGTATCTAGTCCTCCTGAATATGCTAAAACAATTTTCTCTTTCATAAAATTCACCTCATTGTAGTTATCTGAATATTTTGTATGAACACATGAt                                                                              <  1:3662847/100‑1 (MQ=255)            ttAGTCCTCCTGAATATGCTAAAACAATTTTCTCTTTCATAAAATTCACCTCATTGTAGTTATCTGAATATTTCGTATGAACACATGATAGCAAACtttt                                                                   >  1:2388387/2‑100 (MQ=255)              aGTCCTCCTGAATATGCTAAAACAATTTTCTCTTTCATAAAATTCACCTCATTGTAGTTATCTGAATATTTCGTATGAACACATGATAGCAAACTTTTaa                                                                 >  1:2041631/1‑100 (MQ=255)                                                           aCCTCATTGTAGTTATCTGAATATTTCGTATGAACACATGATAGCAAACTTTTAATGAAAATAAAACCATTATTTTACATATTTATTCATAAAATGCGAt                    <  1:2067671/100‑1 (MQ=255)                                                           aCCTCATTGTAGTTATCTGAATATTTCGTATGAACACATGATAGCAAACTTTTAATGAAAATAAAACCATTATTTTACATATTTATTCATAAAATGCGAt                    <  1:4600137/100‑1 (MQ=255)                                                             cTCATTGTAGTTATCTGAATATTTCGTATGAACACATGATAGCAATCTTTTAATGAAAATAAAACCATTATTTTACATATTTATTCATAAAATGCGATag                  <  1:4820931/100‑1 (MQ=255)                                                                  tGTAGTTATCTGAATATTTCGTATGAACACATGATAGCAAACTTTTAATGAAAATAAAACCATTATTTTACATATTTATTCATAAAATGCGAAAGAGAtc             >  1:4288785/1‑99 (MQ=255)                                                                             gAATATTTCGTATGAACACATGATAGCAAACTTTTAATGAAAATAAAACCATTATTTTACATATTTATTCATAAAATGCGATAGAGATAGTTAGAGTTGt  >  1:4422990/1‑100 (MQ=255)                                                                                                 |                                                                                CACACTTGTATCTAGTCCTCCTGAATATGCTAAAACAATTTTCTCTTTCATAAAATTCACCTCATTGTAGTTATCTGAATATTTCGTATGAACACAAGATAGCAAACTTTTAATGAAAATAAAACCATTATTTTACATATTTATTCATAAAATGCGATAGAGATAGTTAGAGTTGT  >  NC_007793/944596‑944771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑