Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	907,626	C→A	L44I (CTA→ATA)	SAUSA300_RS04465 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	907,626	0	C	A	100.0%	40.9 / NA	17	L44I (CTA→ATA)	SAUSA300_RS04465	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/1);  total (16/1)

TTGTCAGCGATTATAGGGTCTATCGTAGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGTTTACCTCGGTGTTGAACATGGATTACTACATAATATTACAACACAAGTAGCGATAGGGACGTCTTCAGTCATTCTAATTGTGACAGGACTTTCTTCATCACTTGGATATTTA  >  NC_007793/907536‑907712                                                                                           |                                                                                       ttGTCAGCGATTATAGGGTCTATCGTAGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGTTTACCTCGGTGTTGAACATGGATTAATACataata                                                                               >  1:1715570/1‑100 (MQ=255)     cAGCGATTATAGGGTCTATCGTAGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGTTTACCTCGGTGTTGAACATGGATTAATACATAATATTac                                                                           >  1:2269034/1‑100 (MQ=255)     cAGCGATTATAGGGTCTATCGTAGGCATTGGAGGCGGTATTATTAACGTTCCAACAATGGTTTACCTCGGAGTTGAACATGGATTAATACATAATATTac                                                                           >  1:2471633/1‑100 (MQ=255)                gggTCTATCGTAGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGTTTACCTCGGTGTTGAACATGGATTAATACATAATATTACAACACAAGTAg                                                                >  1:753192/1‑100 (MQ=255)                gggTCTATCGTAGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGTTTACCTCGGTGTTGAACATGGATTAATACATAATATTACAACACAAGTAg                                                                <  1:5508240/100‑1 (MQ=255)                      aTCGTAGGCATTGGAGGCGGTATTAATATCGTTCCAACAATGGTTTACCTTGGTGTTGAACATGGAATAATTAATAAAATTAAAAAACAAGTAGcagatc                                                          >  1:2224098/1‑95 (MQ=255)                         ctAGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGTTTACCTCGGTGTTGAACATGGATTAATACATAATATTACAACACAAGTAGCGATAGGGa                                                       >  1:532054/2‑100 (MQ=255)                          tAGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGTTTACCTCGGTGTTGAACATGGATTAATACATAATATTACAACACAAGTAGCGCTAGGGAc                                                      >  1:5272644/1‑100 (MQ=255)                          tAGGCATTGGAGGCGGTATTATTATCGGTCCAACAATGGTTTACCTCGGGGTTGAACATGGATTAATACATAATATTACAACACAAGTAGCGATAGGGAc                                                      >  1:3937050/1‑100 (MQ=255)                           aGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGTTTACCTCGGTGTTGAACATGGATTAATACATAATATTACAACACAAGTAGCGATAGGGACg                                                     >  1:3004884/1‑100 (MQ=255)                           aGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGTTTACCTCGGTGTTGAACATGGATTAATACATAATATTACAACACAAGTAGCGATAGGGACg                                                     >  1:2907709/1‑100 (MQ=255)                           aGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGTTTACCTCGGTGTTGAACATGGATTAATACATAATATTACAACACAAGTAGCGATAGGGACg                                                     >  1:66227/1‑100 (MQ=255)                           aGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGGTTATCTCGGTGTTGAACATGGATTAATACATAATATTACAACACAAGTAGCGATAGGGACg                                                     >  1:3640655/1‑100 (MQ=255)                                                         aacaaTGGTTTACCTCGGTGTTGAACATGGATTAATACATAATATTACAACACAAGTAGCGATAGGGACGTCTTCAGTCATTCTAATTGTGACAGGACtt                       >  1:5738834/1‑100 (MQ=255)                                                           caaTGGTTTACCTCGGTGTTGAACATGGATTAATACATAATATTACAACACAAGTAGCGATAGGGACGTCTTCAGTCATTCTAATTGTGACAGGACTttc                     >  1:4728539/1‑100 (MQ=255)                                                                 gtgcccTCGGTGTTGAACATGGATTAATACATAATATTACAACACATGTAGCGATAGGGACGTCTTCAGTCATTCTAATTGTGACAGGACTTTCTtcatc               >  1:1903324/5‑100 (MQ=255)                                                                              tGAACATGGATTAATACATAATATGACAACACAAGTAGCGATAGGGACGTCTTCAGTCATTCTAATTGTGACAGGACTTTCTTCATCACTTGGATATTTa  >  1:5400722/1‑100 (MQ=255)                                                                                           |                                                                                       TTGTCAGCGATTATAGGGTCTATCGTAGGCATTGGAGGCGGTATTATTATCGTTCCAACAATGGTTTACCTCGGTGTTGAACATGGATTACTACATAATATTACAACACAAGTAGCGATAGGGACGTCTTCAGTCATTCTAATTGTGACAGGACTTTCTTCATCACTTGGATATTTA  >  NC_007793/907536‑907712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑