Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	905,735	C→T	P127L (CCT→CTT)	SAUSA300_RS04450 →	nitronate monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	905,735	0	C	T	100.0%	154.9 / NA	54	P127L (CCT→CTT)	SAUSA300_RS04450	nitronate monooxygenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (52/2);  total (52/2)

TATCGACGCGCATTACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCCTGTATGTTGTTTTACTTTTGGAATTCCAAGCGAACAGATTATAAGCAGGTTGAAAGCAGCGAATGTCAAACTTATAGGTACAGCAACAAGTGTT  >  NC_007793/905638‑905829                                                                                                  |                                                                                               tATCGACGCGCATTACATTTAGAAGAGCCGGTTGTTAAACTTACCGAAGAACAACAATTTAACTGTCATCTTGATACGTTAATTAAAAAGCAAGTGCTTg                                                                                              >  1:3124098/1‑100 (MQ=255) tATCGACGCGCATTACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTg                                                                                              >  1:3432109/1‑100 (MQ=255)  cccGACGCGCATTACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCGTATTGATACGATAATTAAAAAGCAAGTGCTTGt                                                                                             >  1:3638835/3‑100 (MQ=255)    cGACGCGCATTACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTAt                                                                                           >  1:159623/1‑100 (MQ=255)     caCGCGCATTACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTAtg                                                                                          >  1:4311649/2‑100 (MQ=255)     caCGCGCATTACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTAtg                                                                                          >  1:5788994/2‑100 (MQ=255)       cgcgCATTACATTTAGAAGAGCCGGTCGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTAtgtt                                                                                        >  1:3585084/1‑100 (MQ=255)         cgcATTACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTAtgttgt                                                                                      >  1:4563414/1‑100 (MQ=255)            gttACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGtttt                                                                                   >  1:1298537/2‑100 (MQ=255)            cttACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGtttt                                                                                   >  1:5650484/2‑100 (MQ=255)            cttACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGtttt                                                                                   >  1:194161/2‑100 (MQ=255)            aTTACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGtttt                                                                                   >  1:1057781/1‑100 (MQ=255)             atACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTa                                                                                  >  1:4104681/2‑100 (MQ=255)              tACATTTTGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:476654/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:5683163/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:3623701/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:5772611/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:5455186/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:4523926/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:5491552/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:2862963/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:2358585/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:97158/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:1826091/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:2992464/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:840697/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:1389764/1‑100 (MQ=255)              tACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTAc                                                                                 >  1:111573/1‑100 (MQ=255)               aCATTTAGATGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACt                                                                                >  1:1916551/1‑100 (MQ=255)               aCATTTAGAAGAGCCGGTTGTAAAACTTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGAGCTTGTATGTTGTTTTACt                                                                                >  1:4072527/1‑100 (MQ=255)               aCATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACt                                                                                >  1:5145390/1‑100 (MQ=255)               aCATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACt                                                                                >  1:837640/1‑100 (MQ=255)               aCATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACt                                                                                >  1:182066/1‑100 (MQ=255)               aCATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACt                                                                                >  1:1701557/1‑100 (MQ=255)               aCATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACt                                                                                >  1:4779262/1‑100 (MQ=255)               aCATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGGGCTTGTATGTTGTTTTACt                                                                                >  1:1501605/1‑100 (MQ=255)                  tttAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACtttt                                                                             >  1:919629/1‑100 (MQ=255)                    cagaagaGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTgg                                                                           >  1:499473/2‑100 (MQ=255)                       aagaGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAAt                                                                        >  1:5080516/1‑100 (MQ=255)                       aagaGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACAATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAAt                                                                        >  1:2680861/1‑100 (MQ=255)                        ctagCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAAtt                                                                       >  1:2182514/3‑100 (MQ=255)                        agagCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAAtt                                                                       >  1:4070374/1‑100 (MQ=255)                          tgCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAATTcc                                                                     >  1:5254325/2‑100 (MQ=255)                          ggCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAATTcc                                                                     >  1:763868/2‑100 (MQ=255)                                        cttACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAATTCCAAGCGAACAGATta                                                       >  1:1445956/2‑100 (MQ=255)                                          aaCCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAATTCCAAGCGAACAGATtata                                                     >  1:797868/2‑100 (MQ=255)                                                  ctcaacaaTTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAATTCCAAGCGAACAGATTATAAGCaagat                                             >  1:2209442/3‑96 (MQ=255)                                                  aacaacAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAATTCCAAGCGAACAGATTATAAGCAGGtt                                             <  1:2027016/100‑1 (MQ=255)                                                     aacaaTTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAATTCCAAGCTAACAGATTATAAGCAGGTTGaa                                          <  1:4894756/100‑1 (MQ=255)                                                                      atTGATACGATAATTAAAAAGCAAGTGCTTGTATGTTGTTTTACTTTTGGAATTCCAAGCGAACAGATTATAAGCAGGTTGAAAGCAGCGAATGTCAAAc                         >  1:3919417/1‑100 (MQ=255)                                                                                        aaGCAAGTGCTTGTATGTTGTTTTACTTTTGGAATTCCAAGCGAACAGATTATAAGCAGGTTGAAAGCAGCGAATGCCAAACTTATAGGTACAGcaacaa       >  1:1403141/1‑100 (MQ=255)                                                                                           ttaGTGCTTGTATGTTGTTTTACTTTTGGAATTCCAAGCGAACAGATTATAAGCAGGTTGAAAGCAGCGAATGTCAAACTTATAGGTACAGCAACAAgtg    >  1:4103857/3‑100 (MQ=255)                                                                                            caGTGCTTGTATGTTGTTTTACTTTTGGAATTCCAAGCGAACAGATTATAAGCAGGTTGAAAGCAGCGAATGTCAAACTTATAGGTACAGCAACAAgtgt   >  1:4130168/2‑100 (MQ=255)                                                                                             ggTGCTTGTATGTTGTTTTACTTTTGGAATTCCAAGCGAACAGATTATAAGCAGGTTGAAAGCAGCGAATGTCAAACTTATAGGTACAGCAACAAGTGtt  >  1:1109724/2‑100 (MQ=255)                                                                                                  |                                                                                               TATCGACGCGCATTACATTTAGAAGAGCCGGTTGTAAAAATTACCGAAGAACAACAATTTAAGTGTCATATTGATACGATAATTAAAAAGCAAGTGCCTGTATGTTGTTTTACTTTTGGAATTCCAAGCGAACAGATTATAAGCAGGTTGAAAGCAGCGAATGTCAAACTTATAGGTACAGCAACAAGTGTT  >  NC_007793/905638‑905829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑