Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	876,937	A→C	Y61S (TAT→TCT)	SAUSA300_RS04280 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	876,937	0	A	C	100.0%	54.1 / NA	22	Y61S (TAT→TCT)	SAUSA300_RS04280	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/0);  total (22/0)

ACCAGTACTACCATGTATTGAAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATATTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCAGAACAAC  >  NC_007793/876845‑877020                                                                                             |                                                                                    aCCAGTACTACCATGTATTGAAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTaaa                                                                              >  1:4855794/1‑100 (MQ=255)          acCATGTATTGAAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCa                                                                     >  1:1476273/1‑100 (MQ=255)          acCATGTATTGAAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCa                                                                     >  1:5045750/1‑100 (MQ=255)            cATGTATTGAAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCAtt                                                                   >  1:1415951/1‑100 (MQ=255)                               aaaaGAGATGCAAGCAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                >  1:5432511/1‑100 (MQ=255)                               aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGTATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                >  1:4073902/1‑100 (MQ=255)                               aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAATTAATGTACCa                                                >  1:3646647/1‑100 (MQ=255)                               aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                >  1:846561/1‑100 (MQ=255)                               aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                >  1:4687968/1‑100 (MQ=255)                               aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                >  1:4564966/1‑100 (MQ=255)                               aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                >  1:4015398/1‑100 (MQ=255)                               aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                >  1:3610515/1‑100 (MQ=255)                               aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                >  1:3517303/1‑100 (MQ=255)                               aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                >  1:3082194/1‑100 (MQ=255)                               aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                >  1:2037968/1‑100 (MQ=255)                               aaaaGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCa                                                >  1:129780/1‑100 (MQ=255)                                   accaTGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATgg                                            >  1:3521619/4‑100 (MQ=255)                                         cAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGc                                      >  1:5315935/1‑100 (MQ=255)                                                           cAGAGTAAGATTGGTGGAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCGAGCACAGAtcggaagag                    >  1:489978/1‑92 (MQ=255)                                                                     ctggtggAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTcc          >  1:4084113/2‑100 (MQ=255)                                                                      tggtggAATGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCa         >  1:4829427/1‑100 (MQ=255)                                                                             aTGCCATTTTTAAAATCTTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCAGaacaac  >  1:1001675/1‑100 (MQ=255)                                                                                             |                                                                                    ACCAGTACTACCATGTATTGAAATGGATCTAAAAGAGATGCAAGAAGAAACATTAAACCAGAGTAAGATTGGTGGAATGCCATTTTTAAAATATTTTAAAGATATACCATTAGATGAAAATAATGTACCAATGGTATTGCTAGCACAGATTAATTTGGATGATCTTCCAGAACAAC  >  NC_007793/876845‑877020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑