Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	867,908	T→C	intergenic (+450/+46)	SAUSA300_RS04190 → / ← SAUSA300_RS04195	cold‑shock protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	867,908	0	T	C	100.0%	57.6 / NA	22	intergenic (+450/+46)	SAUSA300_RS04190/SAUSA300_RS04195	cold‑shock protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/20);  total (2/20)

ACTAATCAACTTTTTGTTAATTTTTTATTAAGACTGATTAATTATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTTATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCTA  >  NC_007793/867811‑868000                                                                                                  |                                                                                             aCTAATCAACTTTTTGTTAATTTTTTATTAAGACTGATTAATTATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTttcat                                                                                            <  1:5706927/100‑1 (MQ=255)           tttttGTTAATTTTTTATTAAGACTGATTAATTATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCg                                                                                  <  1:2640704/100‑1 (MQ=255)           tttttGTTAATTTTTTATTAAGACTGATTAATTATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCg                                                                                  <  1:5620200/100‑1 (MQ=255)                                              tGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGttt                                               <  1:1915649/100‑1 (MQ=255)                                                           tGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTc                                  <  1:3159971/100‑1 (MQ=255)                                                            gTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCggct                                 <  1:5426129/100‑4 (MQ=255)                                                             tttttAAAATCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCaa                                <  1:5020230/100‑1 (MQ=255)                                                             ctcttccgaTCTCATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCaa                                <  1:2536808/92‑1 (MQ=255)                                                                       ctcATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGAGTTTAATTTACAGCGTCAAATATACTTAt                      <  1:2501103/100‑1 (MQ=255)                                                                         cATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTAttt                    >  1:3421901/1‑100 (MQ=255)                                                                         cATAATAATTCAGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTAttt                    >  1:4338542/1‑100 (MQ=255)                                                                                    tccgaTCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg         <  1:4607012/96‑1 (MQ=255)                                                                                    tccgaTCTTGTTTCCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg         <  1:4975180/96‑1 (MQ=255)                                                                                    tccgaTCTTGTATCCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg         <  1:1708847/96‑1 (MQ=255)                                                                                    aGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg         <  1:5192967/100‑1 (MQ=255)                                                                                    aGTAATCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTg         <  1:1994555/100‑1 (MQ=255)                                                                                     ccgaTCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTgg        <  1:1764884/97‑1 (MQ=255)                                                                                        aTCTTGTCTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGt     <  1:2896080/100‑1 (MQ=255)                                                                                         tCTTGTTTTCATTTAAAAGGCGAAACATTAAAGTAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTc    <  1:4822284/100‑1 (MQ=255)                                                                                         tCTTGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTc    <  1:3597694/100‑1 (MQ=255)                                                                                          cTTGTTTCCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCt   <  1:2126999/100‑1 (MQ=255)                                                                                           ttGTTTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCTa  <  1:2811231/100‑1 (MQ=255)                                                                                           ttGTGTTCATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCTa  <  1:577569/100‑1 (MQ=255)                                                                                                  |                                                                                             ACTAATCAACTTTTTGTTAATTTTTTATTAAGACTGATTAATTATTGAGAATATTTATTGTTTTTAAAATCTCATAATAATTCAGTAATCTTGTTTTTATTTAAAAGGCGAAACATTAAAATAATTAAATAAAAATATTGCGTTTAATTTACAGCGTCAAATATACTTATTTCTAATGCTTTGGGGTCTA  >  NC_007793/867811‑868000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑