Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	862,308	A→G	N47D (AAT→GAT)	SAUSA300_RS04170 →	coagulase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	862,308	0	A	G	100.0%	102.7 / NA	37	N47D (AAT→GAT)	SAUSA300_RS04170	coagulase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/1);  total (36/1)

GTATCACAAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAAATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGATTTAATATGGTCCTTTCCAAACTTAGATAATGAAAGATTTGATAATC  >  NC_007793/862212‑862398                                                                                                 |                                                                                           gtATCACAAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATaataaagata                                                                                         >  1:8969/1‑100 (MQ=255) gtATCACAAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATaataaagata                                                                                         >  1:5159452/1‑100 (MQ=255)  tATCACAAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATaataaagataa                                                                                        >  1:5466310/1‑100 (MQ=255)   aTCACAAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATaataaagataaa                                                                                       >  1:4963778/1‑100 (MQ=255)    tgacaAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAagataaat                                                                                      >  1:2632497/3‑100 (MQ=255)    tCACAAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAagataaat                                                                                      >  1:4011159/1‑100 (MQ=255)     cacaAATTTGGGAAAGTAATCGTGCGAGTGCGGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATc                                                                                     >  1:3722906/1‑100 (MQ=255)     cacaAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATc                                                                                     >  1:4744691/1‑100 (MQ=255)     cacaAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATc                                                                                     >  1:2171316/1‑100 (MQ=255)     cacaAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATc                                                                                     >  1:4893312/1‑100 (MQ=255)      acaAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATcc                                                                                    >  1:3493275/1‑99 (MQ=255)          gcatGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCAAGaa                                                                                >  1:1034716/4‑100 (MQ=255)          ctatGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGaa                                                                                >  1:1760040/4‑100 (MQ=255)            ctGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCCAGAACa                                                                              >  1:2779957/2‑100 (MQ=255)            ctGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCAAGAACa                                                                              >  1:2187231/2‑100 (MQ=255)            atGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACa                                                                              >  1:5271968/2‑100 (MQ=255)             tGGGAAAGTAATCGTGCGGGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTGGAACAg                                                                             >  1:205300/1‑100 (MQ=255)             tGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTTGAACAg                                                                             >  1:3599101/1‑100 (MQ=255)             tGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGacaga                                                                             >  1:5635717/1‑96 (MQ=255)              gggAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTTGAACAga                                                                            >  1:1499583/1‑99 (MQ=255)                 aaaGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACagaaga                                                                         >  1:4493792/1‑100 (MQ=255)                      aaTCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAAAACAgatcggaaga                                                                    >  1:5051959/1‑91 (MQ=255)                        ccGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGtat                                                                  >  1:2708465/2‑100 (MQ=255)                        acGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGtat                                                                  >  1:311683/2‑100 (MQ=255)                         cGTGCCAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGtata                                                                 >  1:2125385/1‑100 (MQ=255)                              taGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGaaa                                                            >  1:39833/2‑100 (MQ=255)                               aGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAagagatggaagagc                                                           >  1:4807309/1‑90 (MQ=255)                                gTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAg                                                          >  1:3765498/1‑100 (MQ=255)                                      tGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGga                                                    >  1:1771205/1‑100 (MQ=255)                                        gTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGgatg                                                  <  1:3699151/100‑1 (MQ=255)                                        gTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGgatg                                                  >  1:277663/1‑100 (MQ=255)                                          ggcTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGgatgat                                                >  1:3943699/3‑100 (MQ=255)                                           attGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGAtt                                               >  1:4568740/3‑100 (MQ=255)                                           acTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGAtt                                               >  1:131805/2‑100 (MQ=255)                                            ttGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGAttt                                              >  1:1423129/2‑100 (MQ=255)                                            cTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGAttt                                              >  1:1195608/1‑100 (MQ=255)                                            cTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGAttt                                              >  1:2167404/1‑100 (MQ=255)                                                          ccATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGATTTAATATGGTCCTTTc                                >  1:5284752/1‑100 (MQ=255)                                                                      gAGTCGTTGAAACTGACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGATTTAATATGGTCCTTTCCAAACTTAGATa                    >  1:818119/1‑100 (MQ=255)                                                                                    gACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGATTTAATATGGTCCTTTCCAAACTTAGATAATGAAAGATTTGAt      >  1:1528940/1‑100 (MQ=255)                                                                                    gACTAATAATAAAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGATTTAATATGGTCCTTTCCAAACTTAGATAATGAAAGATTTGAt      >  1:3216369/1‑100 (MQ=255)                                                                                    gACTAATAATAAAGAGAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGATTTAATATGGTCCTTTCCAAACTTAGATAATGAAAGATTTGAt      >  1:2304386/1‑100 (MQ=255)                                                                                        aataataaAGATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGATTTAATATGGTCCTTTCCAAACTTAGATAATGAAAGATTTGATAATc  >  1:5545435/1‑100 (MQ=255)                                                                                                 |                                                                                           GTATCACAAATTTGGGAAAGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTGAAACTGACTAATAATAAAAATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGATGATTTAATATGGTCCTTTCCAAACTTAGATAATGAAAGATTTGATAATC  >  NC_007793/862212‑862398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑