| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 860,929 | G→A | A594A (GCG→GCA) | SAUSA300_RS04165 → | clumping factor A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 860,929 | 0 | G | A | 92.3% | 23.1 / ‑4.4 | 13 | A594A (GCG→GCA) | SAUSA300_RS04165 | clumping factor A |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (9/3); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
GACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCGAGTGATTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGATTCAGATTCAGCAAGCGATTCCGACTCAGC > NC_007793/860837‑861000 | gACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCAAGTgatt > 1:1622331/1‑100 (MQ=255)gACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCAAGTgatt > 1:1909947/1‑100 (MQ=255) cTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGa > 1:5111154/1‑100 (MQ=255) tGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGCAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGAt > 1:4786/1‑100 (MQ=38) ggCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGGTTCAGCAAGTGATTCAGACTCAGCGAGTGAtt > 1:4702388/1‑100 (MQ=38) ggCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGAtt > 1:4022489/1‑100 (MQ=255) gCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGATTc < 1:3510805/100‑1 (MQ=255) aTTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGATTCAGATTc < 1:1952280/100‑1 (MQ=25) tAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGATTCAGATTCAGca < 1:4838311/100‑2 (MQ=21) aaTTCTGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGATTCAGATTCAGcaa > 1:3877488/1‑98 (MQ=21) cAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGATTCAGATTCAGCAAGCGATTCCGACTCAGc > 1:3892353/1‑100 (MQ=14) aTTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCGAGTGATTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGATTCAGATTCAGCAAg < 1:2581383/100‑1 (MQ=255) cTACATCAGATAGTGGTTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGATTCAGATTCAGCAAGCGATTCCGACTCAGCGAGCGATTCCGACTCaga > 1:2512400/1‑99 (MQ=14) | GACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCGAGTGATTCAGATTCAGCAAGTGATTCAGACTCAGCGAGTGATTCAGATTCAGCAAGCGATTCCGACTCAGC > NC_007793/860837‑861000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |