Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	853,311	G→A	E601K (GAA→AAA)	SAUSA300_RS04120 →	ribonuclease R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	853,311	0	G	A	100.0%	123.2 / NA	44	E601K (GAA→AAA)	SAUSA300_RS04120	ribonuclease R
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/1);  total (43/1)

TTTAATCCGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTGAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCAACATATTGGTGAT  >  NC_007793/853229‑853406                                                                                   |                                                                                                 cttAATCCGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGt                                                                                 >  1:2587063/2‑100 (MQ=255)      cccGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcg                                                                            >  1:5040185/2‑100 (MQ=255)       ccGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGACGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcgt                                                                           >  1:2244644/1‑100 (MQ=255)       ccGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcgt                                                                           >  1:3761275/1‑100 (MQ=255)       ccGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcgt                                                                           >  1:5494933/1‑100 (MQ=255)       ccGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcgt                                                                           >  1:5122943/1‑100 (MQ=255)       ccGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcgt                                                                           >  1:5256209/1‑100 (MQ=255)        cGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcgtc                                                                          >  1:1091990/1‑100 (MQ=255)         gTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAAcgtcg                                                                         >  1:448638/1‑100 (MQ=255)                     ttGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGTTATTGAGGc                                                             <  1:3034971/100‑1 (MQ=255)                       gagaAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATAGAGGCAg                                                           >  1:794224/1‑100 (MQ=255)                              cAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGATCggaa                                                    >  1:1547042/1‑97 (MQ=255)                                 tGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATAc                                                 >  1:1875817/1‑100 (MQ=255)                                  ggATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACt                                                >  1:514527/1‑100 (MQ=255)                                  ggATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACt                                                >  1:1808990/1‑100 (MQ=255)                                  ggATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACt                                                >  1:1298998/1‑100 (MQ=255)                                    aTAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACtga                                              >  1:1546336/1‑100 (MQ=255)                                      aaCAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACtgatg                                            >  1:38758/1‑100 (MQ=255)                                         aaaGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGCCGTGCTATTGAGGCAGAACGTGATACTGATGAAt                                         >  1:3421930/1‑100 (MQ=255)                                             aagTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTGAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑aa                                    >  1:2802249/1‑100 (MQ=255)                                             aagTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑aa                                    >  1:3947953/1‑100 (MQ=255)                                              agTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑aaa                                   >  1:1559093/1‑100 (MQ=255)                                               gTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑aaaa                                  >  1:5484168/1‑100 (MQ=255)                                                tGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑aaaaa                                 >  1:5254874/1‑100 (MQ=255)                                                 gAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑aaaaaa                                >  1:2707148/1‑100 (MQ=255)                                                       ttGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAGAAAGCAGat                          >  1:5733372/1‑99 (MQ=255)                                                        tGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGatc                         >  1:869807/1‑98 (MQ=255)                                                        tGGGAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGaaa                         >  1:5595665/1‑99 (MQ=255)                                                         gggAAGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGaaga                        >  1:5607848/1‑98 (MQ=255)                                                             aGACAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATg                    >  1:4878116/1‑100 (MQ=255)                                                              tgcAAATTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTGAAAAAAAGCAGAATATATg                    >  1:1153594/3‑100 (MQ=255)                                                                  ctttGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCa               >  1:943804/3‑100 (MQ=255)                                                                  aaTTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCa               >  1:1492718/1‑100 (MQ=255)                                                                   aTTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCaa              >  1:1038751/1‑100 (MQ=255)                                                                   aTTGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCaa              >  1:643535/1‑100 (MQ=255)                                                                     tGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCAACa            >  1:3816349/1‑100 (MQ=255)                                                                     tGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCAACa            >  1:861620/1‑100 (MQ=255)                                                                     tGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCAACa            >  1:5302223/1‑100 (MQ=255)                                                                     tGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCAACa            >  1:2391433/1‑100 (MQ=255)                                                                     tGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCAACa            >  1:2910739/1‑100 (MQ=255)                                                                     tGCCTGAGTTAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCAACa            >  1:4517840/1‑100 (MQ=255)                                                                              tAGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCAACATATTGGtga   >  1:5305281/1‑100 (MQ=255)                                                                              caGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCAACATATTGGtga   >  1:2894578/2‑100 (MQ=255)                                                                               aGCTAAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCAACATATTGGtgat  >  1:3251818/1‑100 (MQ=255)                                                                                   |                                                                                                 TTTAATCCGTAAGTATTTAATTGAGAAATCAATGGATAACAAAGAAGTGAAGCGTTGGGAAGACAAATTGCCTGAGTTAGCTGAACATACTTCTAAACGTGAACGTCGTGCTATTGAGGCAGAACGTGATACTGATGAATTG‑AAAAAAGCAGAATATATGATTCAACATATTGGTGAT  >  NC_007793/853229‑853406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑