Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	827,290	T→A	H451Q (CAT→CAA)	SAUSA300_RS04005 →	ABC‑ATPase UvrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	827,290	0	T	A	100.0%	113.4 / NA	41	H451Q (CAT→CAA)	SAUSA300_RS04005	ABC‑ATPase UvrA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (41/0);  total (41/0)

TATCTGTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACATGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACAGAG  >  NC_007793/827193‑827384                                                                                                  |                                                                                               tATCTGTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCACGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGa                                                                                              >  1:3451801/1‑100 (MQ=255) tATCTGTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACCATTATAAAAACATTGATTTGTCAGAACAAGa                                                                                              >  1:1387437/1‑100 (MQ=255)   ccTGTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAGCAAGATc                                                                                            >  1:3114794/2‑100 (MQ=255)   ccTGTTTATGTAGGCGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGacc                                                                                            >  1:4729003/2‑98 (MQ=255)    cTGTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCa                                                                                           >  1:1746077/1‑100 (MQ=255)      gTTTATGTGGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAg                                                                                         >  1:2469800/1‑100 (MQ=255)      gTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATGTTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAg                                                                                         >  1:5269918/1‑100 (MQ=255)      gTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGGACTATTATAAAAACATTGATTTGTCGGAACAAGATcgga                                                                                         >  1:5390141/1‑97 (MQ=255)      gTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGGACAAGATcgga                                                                                         >  1:3072853/1‑97 (MQ=255)      gTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGACCAAGATcgga                                                                                         >  1:5608446/1‑97 (MQ=255)      gTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATcgga                                                                                         >  1:3833295/1‑97 (MQ=255)      aattATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAg                                                                                         >  1:4300868/3‑100 (MQ=255)         tATGTAGGTGGTTTAAATATTGGCGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGa                                                                                      >  1:4516897/1‑100 (MQ=255)          aTGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGAt                                                                                     >  1:528374/1‑100 (MQ=255)          aTGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGACAGAACAAGATCAAGCGAt                                                                                     >  1:1138306/1‑100 (MQ=255)            gTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATGTTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTg                                                                                   >  1:282281/1‑100 (MQ=255)            gTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATcggaagagca                                                                                   >  1:1564675/1‑91 (MQ=255)            gTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTg                                                                                   >  1:2122222/1‑100 (MQ=255)            gTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTg                                                                                   >  1:2282225/1‑100 (MQ=255)            gTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTg                                                                                   >  1:4494986/1‑100 (MQ=255)                  ggtTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGcaaatc                                                                             >  1:4397607/1‑100 (MQ=255)                              ggTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTgaaag                                                                 >  1:3112625/1‑100 (MQ=255)                                 aaaGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTgaaagaaa                                                              >  1:4466677/2‑100 (MQ=255)                                              ctccAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACt                                                 >  1:5443194/4‑100 (MQ=255)                                                         cAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTaa                                      >  1:3119701/1‑100 (MQ=255)                                                          atctccTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTaaa                                     >  1:2297361/6‑100 (MQ=255)                                                              gcTGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAaataat                                 >  1:5505722/1‑100 (MQ=255)                                                                tGAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAAtgt                               >  1:3326537/1‑100 (MQ=255)                                                                 gAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAAtgtg                              >  1:1023080/1‑100 (MQ=255)                                                                 gAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAAtgtg                              >  1:1588408/1‑100 (MQ=255)                                                                 gAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAAtgtg                              >  1:5296633/1‑100 (MQ=255)                                                                 gAACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAAtgtg                              >  1:1108933/1‑100 (MQ=255)                                                                   aCTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTggg                            >  1:3525729/1‑100 (MQ=255)                                                                                      ggaggcAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTa         >  1:1470225/6‑100 (MQ=255)                                                                                          tCAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAGACa     >  1:3834792/1‑100 (MQ=255)                                                                                           cAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACag    >  1:1467747/1‑100 (MQ=255)                                                                                           cAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACag    >  1:1080053/1‑100 (MQ=255)                                                                                           cAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACCTTTTTAAATAATGTGGGACTTGAATATTTAACGTTagatcg    >  1:5140559/1‑95 (MQ=255)                                                                                           cAGAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACCTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACag    >  1:4648772/1‑100 (MQ=255)                                                                                            cgAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACCTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACaga   >  1:1392995/2‑100 (MQ=255)                                                                                             gAACAAGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACagag  >  1:103602/1‑100 (MQ=255)                                                                                                  |                                                                                               TATCTGTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTGAACTATTATAAAAACATTGATTTGTCAGAACATGATCAAGCGATTGCAAATCAAATATTGAAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGTTAAACAGAG  >  NC_007793/827193‑827384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑