Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	806,587	T→C	Y207H (TAT→CAT)	SAUSA300_RS03910 →	glycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	806,587	0	T	C	100.0%	155.9 / NA	58	Y207H (TAT→CAT)	SAUSA300_RS03910	glycerate kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/6);  total (52/6)

ACACCCTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGTATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGAAAATTGCAATTGGTCCAG  >  NC_007793/806490‑806683                                                                                                  |                                                                                                 ccacCCTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCAt                                                                                                >  1:1565054/2‑100 (MQ=255) acacCCTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCAt                                                                                                >  1:1037329/1‑100 (MQ=255) acacCCTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCAt                                                                                                >  1:3653288/1‑100 (MQ=255) acacCCTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCAt                                                                                                >  1:3296816/1‑100 (MQ=255) acacCCTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCAt                                                                                                >  1:3109782/1‑100 (MQ=255) acacCCTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCAt                                                                                                >  1:193374/1‑100 (MQ=255) acacCCTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCAt                                                                                                >  1:1906137/1‑100 (MQ=255)  cacCCTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATc                                                                                               >  1:4496917/1‑100 (MQ=255)  cacCCTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATc                                                                                               >  1:3315657/1‑100 (MQ=255)    cccTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAg                                                                                             >  1:3266775/1‑100 (MQ=255)        aaaaTGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGa                                                                                         >  1:1161316/1‑100 (MQ=255)        aaaaTGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGa                                                                                         >  1:38509/1‑100 (MQ=255)        aaaaTGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGa                                                                                         >  1:1990182/1‑100 (MQ=255)        aaaaTGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGa                                                                                         >  1:5706208/1‑100 (MQ=255)        aaaaTGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGa                                                                                         >  1:2372611/1‑100 (MQ=255)        aaaaTGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGa                                                                                         >  1:241794/1‑100 (MQ=255)        aaaaTGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTCGa                                                                                         >  1:410919/1‑100 (MQ=255)         acaTGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGaa                                                                                        >  1:4593363/3‑100 (MQ=255)          cttGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAAt                                                                                       >  1:1598895/3‑100 (MQ=255)            agcacaCAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCa                                                                                     >  1:1803281/6‑100 (MQ=255)             ggAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCAt                                                                                    >  1:4591010/1‑100 (MQ=255)               aaaCAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATaa                                                                                  >  1:3642507/1‑100 (MQ=255)               aaaCAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATaa                                                                                  >  1:2268763/1‑100 (MQ=255)               aaaCAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACCTATGATGCGCATCAGTTGAATCATaa                                                                                  >  1:5224932/1‑100 (MQ=255)                taCAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAAt                                                                                 >  1:2240696/2‑100 (MQ=255)                caCAGCAAGTATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAAt                                                                                 >  1:4376818/2‑100 (MQ=255)                 aCAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCGAACTTATGATGCGCATCAGTTGAATCATAATc                                                                                >  1:2883228/1‑100 (MQ=255)                 aCAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAAtt                                                                                >  1:1535577/1‑99 (MQ=255)                 aCAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATc                                                                                >  1:3116247/1‑100 (MQ=255)                 aCAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATc                                                                                >  1:491046/1‑100 (MQ=255)                 aCAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGAGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATc                                                                                >  1:3533295/1‑100 (MQ=255)                  cAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCTTGCAAACTTATGATGCGCATCAGTTGAATCATAATCa                                                                               >  1:1910624/1‑100 (MQ=255)                  cAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCa                                                                               >  1:3959216/1‑100 (MQ=255)                  cAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCa                                                                               >  1:5176744/1‑100 (MQ=255)                  cAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCa                                                                               >  1:393659/1‑100 (MQ=255)                      aaGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAggca                                                                           >  1:847386/1‑100 (MQ=255)                       aGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAagcac                                                                          >  1:2258626/1‑99 (MQ=255)                        gAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAagcagc                                                                         <  1:549112/100‑1 (MQ=255)                         aaTTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAagcagcg                                                                        <  1:4959959/100‑2 (MQ=255)                          gttCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAagcagcta                                                                       >  1:1573647/2‑98 (MQ=255)                          aTTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAagcagcgg                                                                       <  1:4788101/100‑3 (MQ=255)                          aTTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGcagcag                                                                       <  1:1516300/100‑1 (MQ=255)                             cAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGaga                                                                    >  1:3364209/1‑98 (MQ=255)                                     gTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGaaaatacaaat                                                            <  1:1255780/100‑9 (MQ=255)                                       cGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGAtaattt                                                          >  1:449625/1‑100 (MQ=255)                                                          gtatGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTGATTTGGTATTTTAGTGAg                                       >  1:1115307/2‑100 (MQ=255)                                                             tGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTa                                    >  1:2487362/1‑100 (MQ=255)                                                             tGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTa                                    >  1:1644374/1‑100 (MQ=255)                                                              ggTAAGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTAt                                   >  1:5091065/1‑100 (MQ=255)                                                                 aaGCAAAGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGCGAGTTATTTa                                >  1:3551582/1‑100 (MQ=255)                                                                       aGTGAAATCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTg                          <  1:2983076/100‑1 (MQ=255)                                                                            aaTCATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTg                     >  1:1237836/1‑100 (MQ=255)                                                                               cATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGaaa                  >  1:334876/1‑100 (MQ=255)                                                                                aTGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGaaaa                 >  1:344368/1‑100 (MQ=255)                                                                                            gatgCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGAAAATTGCAATTGGTc     >  1:4627968/1‑100 (MQ=255)                                                                                             ctgCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGAAAATTGCAATTGGTcc    >  1:2849694/2‑100 (MQ=255)                                                                                             atgCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGAAAATTGCAATCGGTcc    >  1:22457/1‑100 (MQ=255)                                                                                              tgCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGAAAATTGCAATTGGTCCa   >  1:5474680/1‑100 (MQ=255)                                                                                               gcgcATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGAAAATTGCAATTGGTCCAg  >  1:5654107/1‑100 (MQ=255)                                                                                               gcgcATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGAAAATTGCAATTGGTCCAg  >  1:4279432/1‑100 (MQ=255)                                                                                                  |                                                                                                 ACACCCTAAAATGGAAACAGCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATCATGCAAACTTATGATGCGTATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTAATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGAAAATTGCAATTGGTCCAG  >  NC_007793/806490‑806683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑