Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	781,530	C→T	Q93* (CAA→TAA)	SAUSA300_RS03790 →	ATP‑dependent DNA helicase RecQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	781,530	0	C	T	100.0%	156.1 / NA	54	Q93* (CAA→TAA)	SAUSA300_RS03790	ATP‑dependent DNA helicase RecQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/8);  total (46/8)

CCACTAATATCATTAATGAAAGATCAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAACAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTTTTAAATATGCTTCA  >  NC_007793/781434‑781624                                                                                                 |                                                                                               ccACTAATATCATTAATGAAAGATCAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCaataac                                                                                             >  1:308098/1‑100 (MQ=255)   aCTAATATCATTAATGAAAGATCAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGt                                                                                           <  1:2727833/100‑1 (MQ=255)         atCATTAATGAAAGATCAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGaa                                                                                     >  1:2174747/1‑100 (MQ=255)                   aaaGATCAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTAt                                                                           >  1:3584202/1‑100 (MQ=255)                      gctCAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCaa                                                                        >  1:1189901/3‑100 (MQ=255)                       ctCAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCaaa                                                                       >  1:4054855/2‑100 (MQ=255)                       ctCAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCaaa                                                                       >  1:1951945/2‑100 (MQ=255)                        ctcaGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAAt                                                                      >  1:4567407/4‑100 (MQ=255)                        ccAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAAt                                                                      >  1:1158288/2‑100 (MQ=255)                         cAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATg                                                                     >  1:3528120/1‑100 (MQ=255)                         cAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATg                                                                     >  1:4713487/1‑100 (MQ=255)                          aaGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATgg                                                                    <  1:264231/100‑1 (MQ=255)                          aaGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATgg                                                                    <  1:3531508/100‑1 (MQ=255)                          aaGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATgg                                                                    >  1:5243650/1‑100 (MQ=255)                          aaGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATgg                                                                    <  1:1953936/100‑1 (MQ=255)                          aaGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTAACAAATgg                                                                    <  1:449311/100‑1 (MQ=255)                           aGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGga                                                                   >  1:5536064/1‑100 (MQ=255)                           aGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGga                                                                   >  1:8067/1‑100 (MQ=255)                            gTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGgag                                                                  <  1:366281/100‑1 (MQ=255)                            atGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGgag                                                                  >  1:575551/2‑100 (MQ=255)                                  cAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTc                                                            >  1:4893736/1‑100 (MQ=255)                                  cAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTc                                                            >  1:1197473/1‑100 (MQ=255)                                    aTTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCaa                                                          >  1:1276893/1‑100 (MQ=255)                                      tAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAAtt                                                        >  1:4458095/1‑100 (MQ=255)                                      tAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAAtt                                                        >  1:5462825/1‑100 (MQ=255)                                      tAAAAGCGATGGGAACTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAAtt                                                        >  1:3343419/1‑100 (MQ=255)                                       taaaGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAAttt                                                       >  1:81889/2‑100 (MQ=255)                                       taaaGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAAttt                                                       >  1:2032463/2‑100 (MQ=255)                                       aaaaGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAATAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAAttt                                                       >  1:3260259/1‑100 (MQ=255)                                        caaGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAAtttt                                                      >  1:1990304/2‑100 (MQ=255)                                        aaaGCGACGGGAATTCAAGCTGCTTTTTTAAATAGTAGTATGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATCATCAAATGGAGAAATTCAAtttt                                                      >  1:2864246/1‑100 (MQ=255)                                         aaGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAAttttt                                                     >  1:1453181/1‑100 (MQ=255)                                         aaGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAAttttt                                                     >  1:230807/1‑100 (MQ=255)                                          aGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTg                                                    >  1:3016112/1‑100 (MQ=255)                                           tcGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGt                                                   >  1:5726492/2‑100 (MQ=255)                                           gCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGt                                                   >  1:1169196/1‑100 (MQ=255)                                           gCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGt                                                   >  1:1652744/1‑100 (MQ=255)                                            cGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTa                                                  >  1:1532235/1‑100 (MQ=255)                                            cGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTa                                                  >  1:1652980/1‑100 (MQ=255)                                              aTGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATg                                                <  1:4437513/100‑1 (MQ=255)                                              aTGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATg                                                >  1:4982930/1‑100 (MQ=255)                                                gggAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGtc                                              >  1:825543/1‑99 (MQ=255)                                                gggAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTCTTGTATGtc                                              >  1:343332/1‑99 (MQ=255)                                                          gctgctTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAAc                                    <  1:849859/100‑1 (MQ=255)                                                                    tAAATAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAAcagatcggaag                          >  1:3453599/1‑90 (MQ=255)                                                                     aaaTAGTAGTTTGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGATCggaagagcaca                         >  1:1848257/1‑90 (MQ=255)                                                                                tGACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATAtttttt              >  1:4950083/1‑100 (MQ=255)                                                                                 gACTCAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTTTTa             >  1:2222408/1‑100 (MQ=255)                                                                                     cAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTTTTAAata         >  1:3124633/1‑100 (MQ=255)                                                                                     cAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTTTTAAata         >  1:2003034/1‑100 (MQ=255)                                                                                     cAAAAAGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTTTTAAata         >  1:1301774/1‑100 (MQ=255)                                                                                       aaaaGAGCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTTTTAAATATg       >  1:4672149/1‑100 (MQ=255)                                                                                         cagagCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTTTTAAATATGCt     >  1:181122/2‑100 (MQ=255)                                                                                            agCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTTTTAAATATGCTTCa  >  1:3935702/1‑100 (MQ=255)                                                                                            agCAATAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTTTTAAATATGCTTCa  >  1:4092549/1‑100 (MQ=255)                                                                                                 |                                                                                               CCACTAATATCATTAATGAAAGATCAAGTGGATCAATTAAAAGCGATGGGAATTCAAGCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAACAACGTATTGAAAAAGCATTATCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTTTTAAATATGCTTCA  >  NC_007793/781434‑781624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑