Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	757,599	T→C	T121T (ACT→ACC)	SAUSA300_RS03665 →	DeoR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	757,599	0	T	C	100.0%	40.0 / NA	17	T121T (ACT→ACC)	SAUSA300_RS03665	DeoR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/0);  total (17/0)

TCAACGATAATGAATGCTTATTTATCGATGCTGGTTCATCTACATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACTAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACAATTATGCTAGGTGGTCAAGTTAAAG  >  NC_007793/757508‑757672                                                                                            |                                                                          tcaaCGATAATGAATGCTTATTTATCGATGCTGGTTCATCTACATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGttt                                                                   >  1:3994559/1‑100 (MQ=255)                            aTGCTGGTTCATCTACATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTaaaaa                                        >  1:5424417/1‑100 (MQ=255)                             tGCTGGTTCATCTACATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTaaaaaa                                       >  1:1036335/1‑100 (MQ=255)                              gCTGGTTCATCTACATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAg                                      >  1:2625241/1‑100 (MQ=255)                              gCTGGTTCATCTACATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAg                                      >  1:3538738/1‑100 (MQ=255)                                   atCATCTACATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTAtt                                 >  1:5038283/2‑100 (MQ=255)                                     cATCTACATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTaa                               >  1:2307764/1‑100 (MQ=255)                                          aCATTGGAGCTAATTAAATATATTCAAGCGACAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACaa                          >  1:4174592/1‑100 (MQ=255)                                          aCATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACaa                          >  1:1210080/1‑100 (MQ=255)                                          aCATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACaa                          >  1:854068/1‑100 (MQ=255)                                          aCATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACaa                          >  1:5337796/1‑100 (MQ=255)                                          aCATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACaa                          >  1:4897050/1‑100 (MQ=255)                                          aCATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACaa                          >  1:2607463/1‑100 (MQ=255)                                          aCATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGAGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACat                          >  1:5002825/1‑99 (MQ=255)                                               ggAGCTAATTAAATATATTCAAGCGAAAGATATCATTGCGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACAATTATg                     >  1:827322/1‑100 (MQ=255)                                                                ttCAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACAATTATGCTAGGTGGTCAAGTTaa    >  1:4366070/1‑100 (MQ=255)                                                                  cAAGCGAAAGATATCATTGTGGTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACAATTATGCTAGGTGGTCAAGTTAAAg  >  1:554932/1‑100 (MQ=255)                                                                                            |                                                                          TCAACGATAATGAATGCTTATTTATCGATGCTGGTTCATCTACATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTGGTAACTAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACAATTATGCTAGGTGGTCAAGTTAAAG  >  NC_007793/757508‑757672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑