Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	757,384	C→T	L50L (CTG→TTG)	SAUSA300_RS03665 →	DeoR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	757,384	0	C	T	100.0%	30.5 / NA	12	L50L (CTG→TTG)	SAUSA300_RS03665	DeoR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/1);  total (11/1)

TTACAAGAATTAATAGATCGAACTGGTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAACTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCTTGATGAAAAGAA  >  NC_007793/757303‑757478                                                                                  |                                                                                               ttACAAGAATTAATAGATCGAACTGGTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCAtggtc                                                                              <  1:5002348/100‑2 (MQ=255)                     aaCTGGTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAAt                                                          >  1:3123036/1‑100 (MQ=255)                     aaCTGGTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAAt                                                          >  1:4234401/1‑100 (MQ=255)                         ggTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATTTTAAAAGAAAATCGTa                                                      >  1:5272242/1‑100 (MQ=255)                         ggTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTa                                                      >  1:1210138/1‑100 (MQ=255)                         ggTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTa                                                      >  1:4208560/1‑100 (MQ=255)                         ggTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTa                                                      >  1:4251904/1‑100 (MQ=255)                            ggCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATgg                                                   >  1:2622147/2‑100 (MQ=255)                                                             aaaCTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGa                  >  1:5149017/1‑100 (MQ=255)                                                                  acaacaATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCtt             >  1:4036571/1‑100 (MQ=255)                                                                           aGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCTTGATgaaaag    >  1:5274643/1‑100 (MQ=255)                                                                             ggAAATTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCTTGATgaaaagaa  >  1:4906965/1‑100 (MQ=255)                                                                                  |                                                                                               TTACAAGAATTAATAGATCGAACTGGTTGCAGTGCTTCAACAATACGAAGAGATTTATCTAAACTACAACAATTAGGGAAACTGCAACGTGTGCATGGTGGTGCAATGTTAAAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCTTGATGAAAAGAA  >  NC_007793/757303‑757478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑