Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	740,015	A→G	Y159H (TAT→CAT)	SAUSA300_RS03575 ←	lysine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	740,015	0	A	G	100.0%	65.5 / NA	26	Y159H (TAT→CAT)	SAUSA300_RS03575	lysine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/19);  total (7/19)

ATGATTAATCGTATGAACGAGTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATACTTAGGATCAATAAAACCTTCCTCAATCATATGGTCAATCATTGTTTGTAGTGGATTGAAAAAGCCATTAATATTATAAATGGCAATAGGCTTT  >  NC_007793/739919‑740109                                                                                                 |                                                                                               aTGATTAATCGTATGAACGAGTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCtt                                                                                             <  1:646340/100‑1 (MQ=255) aTGATTAATCGTATGAACGAGTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCtt                                                                                             <  1:5776454/100‑1 (MQ=255) aTGATTAATCGTATGAACGAGTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCtt                                                                                             <  1:1850789/100‑1 (MQ=255)  tGATTAATCGTATGAACGAGTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCttg                                                                                            <  1:1725556/100‑2 (MQ=255)  tGATTAATCGTATGAACGAGTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTa                                                                                            <  1:2897442/100‑1 (MQ=255)   gATTAATCGTATGAACGAGTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAg                                                                                           <  1:3041616/100‑1 (MQ=255)    aTTAATCGTATGAACGAGTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAgg                                                                                          <  1:2028638/100‑1 (MQ=255)               gAACGAGTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAAc                                                                               >  1:1275394/1‑100 (MQ=255)                aaCGAGTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAAcc                                                                              >  1:3891933/1‑100 (MQ=255)                    ggTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTcc                                                                          >  1:5641790/2‑100 (MQ=255)                       aCCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCa                                                                       >  1:3476401/1‑100 (MQ=255)                        cccAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCag                                                                      <  1:3516119/100‑2 (MQ=255)                        accAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCaa                                                                      >  1:223785/2‑100 (MQ=255)                         ccAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCAAt                                                                     <  1:5452676/100‑1 (MQ=255)                                  tGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCAATCATATggag                                                            <  1:1526396/100‑3 (MQ=255)                                  tGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCAATCATATGGTc                                                            <  1:2256713/100‑1 (MQ=255)                                  tGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCAATCATATGGTc                                                            <  1:1709169/100‑1 (MQ=255)                                   gAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCAATCATATGGTcg                                                           <  1:1930241/100‑2 (MQ=255)                                   gAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCAATCATATGGTCa                                                           <  1:3120998/100‑1 (MQ=255)                                   gAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCAATCATATGGTCa                                                           <  1:3732272/100‑1 (MQ=255)                                    aaaTTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCAATCATATGGTCaa                                                          <  1:305212/100‑1 (MQ=255)                                                    cAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCAATCATATGGTCAATCATTGTTTGTAGTgg                                          <  1:5454181/100‑1 (MQ=255)                                                     aaTTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATGCTTAGGATCAATAAAACCTTCCTCAATCATATGGTCAATCATTGTTTGTAGTggg                                         <  1:3323192/100‑2 (MQ=255)                                                                                          gCACGATGCTTAGGATCAATAAAACCTTCCTCAATCATATGGTCAATCATTGTTTGTAGTGGATTGAAAAAGCCATTAATATTATAAATGGCAATAGGCt    <  1:1477236/100‑1 (MQ=255)                                                                                          ccACGATGCTTAGGATCAATAAAACCTTCCTCAATCATATGGTCAATCATTGTTTGTAGTGGATTGAAAAAGCCATTAATATTATAAATGTCAATAGGCt    >  1:1399486/2‑100 (MQ=255)                                                                                            aCGATGCTTAGGATCAATAAAACCTTCCTCAATCATATGGTCAATCATTGTTTGTAGTGGATTGAAAAAGCCATTAATATTATAAATGGCAATAGGCttt  >  1:3070969/1‑100 (MQ=255)                                                                                                 |                                                                                               ATGATTAATCGTATGAACGAGTACCCAGAGGTTTGAAATTTAATATTGATTCAATTAATGATTCCTTAGTGTCGCATAACGGTGCAAGAGCACGATACTTAGGATCAATAAAACCTTCCTCAATCATATGGTCAATCATTGTTTGTAGTGGATTGAAAAAGCCATTAATATTATAAATGGCAATAGGCTTT  >  NC_007793/739919‑740109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑