Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	735,887	G→A	M215I (ATG→ATA)	SAUSA300_RS03540 →	sugar MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	735,887	0	G	A	100.0%	109.9 / NA	40	M215I (ATG→ATA)	SAUSA300_RS03540	sugar MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/2);  total (38/2)

TACTTTAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATGTTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTGTTA  >  NC_007793/735794‑735981                                                                                              |                                                                                               taCTTTAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTaaa                                                                                          >  1:4428323/1‑100 (MQ=255)     ttAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGatc                                                                                      >  1:3126038/1‑98 (MQ=255)        gTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACaaag                                                                                   <  1:2991489/100‑2 (MQ=255)                           ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                                >  1:961702/1‑100 (MQ=255)                           ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                                >  1:733284/1‑100 (MQ=255)                           ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                                >  1:5171234/1‑100 (MQ=255)                           ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                                >  1:2109864/1‑100 (MQ=255)                           ataAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTAt                                                                >  1:266806/1‑100 (MQ=255)                                    taaacattaaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCatttat                                                       >  1:592877/1‑100 (MQ=255)                                    taaacattaaacaCCCTATTAGTACGCAACAACATGTTGAAAAAAGTGCTCCTAATATATTTAAAGACAAAACGCTTTTATGACCATTTATTGCatttat                                                       >  1:4948194/1‑100 (MQ=255)                                     aaacattaaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCatttatt                                                      >  1:3956772/1‑100 (MQ=255)                                           taaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTGCCATTTATTGCATTTATTttatta                                                >  1:3482745/1‑100 (MQ=255)                                           taaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTttatta                                                >  1:3371312/1‑100 (MQ=255)                                           taaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTttatta                                                >  1:4716262/1‑100 (MQ=255)                                           taaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTttatta                                                >  1:4049664/1‑100 (MQ=255)                                           taaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTttatta                                                >  1:3011703/1‑100 (MQ=255)                                           taaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTttatta                                                >  1:2571114/1‑100 (MQ=255)                                           acgacacCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTttatta                                                >  1:381154/4‑100 (MQ=255)                                            gcacacCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTCTATTac                                               >  1:2735597/3‑100 (MQ=255)                                            aaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTac                                               >  1:4467797/1‑100 (MQ=255)                                            aaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTac                                               >  1:952512/1‑100 (MQ=255)                                            aaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTac                                               >  1:551478/1‑100 (MQ=255)                                            aaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTac                                               >  1:4995517/1‑100 (MQ=255)                                            aaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTac                                               >  1:4510980/1‑100 (MQ=255)                                            aaacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTac                                               >  1:1020491/1‑100 (MQ=255)                                             aacaCCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTaca                                              >  1:1097301/1‑100 (MQ=255)                                              acacCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTacac                                             >  1:3899054/1‑100 (MQ=255)                                              acacCCTATTAGTACGCAACAACATGTTGAAAAAATTACTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTacac                                             >  1:1645024/1‑100 (MQ=255)                                                       tAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACACTGGACag                                    >  1:2282720/1‑99 (MQ=255)                                                        ggTACGCAACAACATGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAAt                                   >  1:2528596/2‑100 (MQ=255)                                                             gCAACAACATGTTGAAAAAATTGCTCCTCATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGActagatcgg                              >  1:2508647/1‑92 (MQ=255)                                                                ccaacaTGTTGAAAAAATTGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGtat                           >  1:1302304/2‑100 (MQ=255)                                                                                 gggCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCttt          >  1:1981896/3‑100 (MQ=255)                                                                                  tGCTCCTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTa         >  1:1526267/1‑100 (MQ=255)                                                                                      ccTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTg     >  1:3697633/1‑100 (MQ=255)                                                                                      ccTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTg     >  1:2027093/1‑100 (MQ=255)                                                                                      ccTAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTg     >  1:3342735/1‑100 (MQ=255)                                                                                        tAATATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTGtt   <  1:2056986/100‑1 (MQ=255)                                                                                         aaTATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTGTTa  >  1:2137311/1‑100 (MQ=255)                                                                                         aaTATATTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTGTTa  >  1:5308760/1‑100 (MQ=255)                                                                                              |                                                                                               TACTTTAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACACCCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATGTTTAAAGACAAAACGCTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATGAATATGCCTTTATTTGTTA  >  NC_007793/735794‑735981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑