Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	731,434	A→G	D60G (GAT→GGT)	SAUSA300_RS03505 →	transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	731,434	0	A	G	100.0%	92.5 / NA	34	D60G (GAT→GGT)	SAUSA300_RS03505	transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/0);  total (34/0)

AAATGATTTAGCAGTCTTAGATTTAACGATGAAGCATTGCAAAGATGAAAAAGTACTTATGCAATCATTTTTAAAAACTGCAATGGATGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATGAG  >  NC_007793/731348‑731528                                                                                       |                                                                                               aaaTGATTTAGCAGTCTTAGATTTAACGATGAAGCATTGCAAAGATGAAAAAGTACTTATGCAATCATTTTTAAAAACTGCAATGGGAGAGCTAGAtcgg                                                                                   >  1:3060837/1‑97 (MQ=255)     gATTTAGCAGTCTTAGATTTAACGATGAAGCATTGCAAAGATGAAAAAGTACTTATGCAATCATTTTTAAAAACTGCAATGGGTGAGCTAGATTTAAGTa                                                                               >  1:1428270/1‑100 (MQ=255)                                                        cTTATGCAATCATTTTTAAAAACTGCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTa                            >  1:2044384/1‑100 (MQ=255)                                                                    taaggctgagcTGCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGat                >  1:4536455/11‑100 (MQ=255)                                                                        caaaaaCTGCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGatcatc            >  1:2904985/2‑100 (MQ=255)                                                                           tgtgtGCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAATGAAAGACTTAGTAAAGTTAGATCATCTaa         >  1:4190629/5‑100 (MQ=255)                                                                           aaaCTGCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTCAGatcggaaga         >  1:5024970/1‑94 (MQ=255)                                                                            aaCTGCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTaaa        >  1:2001298/1‑100 (MQ=255)                                                                             tcTGCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAg       >  1:542478/2‑100 (MQ=255)                                                                             aCTGCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAg       >  1:368042/1‑100 (MQ=255)                                                                             aCTGCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAg       >  1:1974589/1‑100 (MQ=255)                                                                             aCTGCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAg       >  1:2051847/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTACGCAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAATGAAAGACTTAGCAAAGTTAGATCAGCTAAAGAtc    >  1:4331910/1‑99 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTCATTGAAAAAGAAAGACTTCGTAATGTTAGATCATCTAAAGATg    >  1:1357200/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGacg    >  1:543567/1‑98 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:5157009/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:476378/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:562287/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:830497/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:1045863/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:870369/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:389264/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:3692840/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:3674440/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:3426505/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:3095252/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:2486589/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:2236303/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:2212490/1‑100 (MQ=255)                                                                                gCAATGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:1729566/1‑100 (MQ=255)                                                                                accaTGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATg    >  1:5604260/4‑100 (MQ=255)                                                                                 cgaTGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATGa   >  1:2129114/3‑100 (MQ=255)                                                                                  taTGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATGAg  >  1:1759910/2‑100 (MQ=255)                                                                                  aaTGGGTGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATGAg  >  1:3452065/1‑100 (MQ=255)                                                                                       |                                                                                               AAATGATTTAGCAGTCTTAGATTTAACGATGAAGCATTGCAAAGATGAAAAAGTACTTATGCAATCATTTTTAAAAACTGCAATGGATGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAAAAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATGAG  >  NC_007793/731348‑731528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑