Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	718,921	T→C	I281I (ATT→ATC)	SAUSA300_RS03455 →	3‑beta hydroxysteroid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	718,921	0	T	C	100.0%	191.6 / NA	71	I281I (ATT→ATC)	SAUSA300_RS03455	3‑beta hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (70/1);  total (70/1)

ATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATTCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTAAATGATATTGGGTGATATGG  >  NC_007793/718849‑719017                                                                         |                                                                                                 ataCGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTTAAGTTGACTGCCGGAtt                                                                       >  1:1553587/1‑100 (MQ=255) ataCGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGAtt                                                                       >  1:442104/1‑100 (MQ=255) ataCGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGAtt                                                                       >  1:1053471/1‑100 (MQ=255) ataCGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGAtt                                                                       >  1:888978/1‑100 (MQ=255) ataCGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGAtt                                                                       >  1:3739574/1‑100 (MQ=255) ataCGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGAtt                                                                       >  1:695985/1‑100 (MQ=255) ataCGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGAtt                                                                       >  1:5187695/1‑100 (MQ=255) ataCGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGAtt                                                                       >  1:2556635/1‑100 (MQ=255) ataCGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCAGAtc                                                                       >  1:510006/1‑99 (MQ=255)   aCGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTaa                                                                     <  1:5496881/100‑1 (MQ=255)         atatCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGAttt                                                               >  1:1235647/1‑100 (MQ=255)           atCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCGCCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTc                                                             >  1:934740/1‑100 (MQ=255)           atCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACTTACGCAATATATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTc                                                             >  1:4195767/1‑100 (MQ=255)           atCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTc                                                             >  1:3651381/1‑100 (MQ=255)           atCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTc                                                             >  1:4154449/1‑100 (MQ=255)             ccttCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGATGACTGCCGGATTAAAGATTTTCaa                                                           >  1:2470826/4‑100 (MQ=255)             cccTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCaa                                                           >  1:5320112/1‑100 (MQ=255)             cccTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCaa                                                           >  1:1821458/1‑100 (MQ=255)               cTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAg                                                         >  1:2102697/1‑100 (MQ=255)                 cGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTc                                                       >  1:127538/1‑100 (MQ=255)                     tattTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGAtt                                                   >  1:3323634/1‑100 (MQ=255)                        tttGGCCGATTTATTACTTTATACAAGCACCAATGCCAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTaaa                                                >  1:2580246/1‑100 (MQ=255)                        tttGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTaaa                                                >  1:1186196/1‑100 (MQ=255)                         ttGGCCGATTTATTACTTTATACAAGCACCAATGCAAATAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTaaaa                                               >  1:5022361/1‑100 (MQ=255)                         ttGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTaaaa                                               >  1:4493036/1‑100 (MQ=255)                         ttGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTaaaa                                               >  1:2401393/1‑100 (MQ=255)                          tGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGGTGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAAt                                              >  1:1021433/1‑100 (MQ=255)                          tGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAAt                                              >  1:5049784/1‑100 (MQ=255)                          tGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAAt                                              >  1:5269305/1‑100 (MQ=255)                          tGGCCGATTTATTAC‑TTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATc                                             >  1:2820357/1‑100 (MQ=255)                           ggCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTaaagcc                                             >  1:2740833/1‑97 (MQ=255)                           ggCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATc                                             >  1:4594332/1‑100 (MQ=255)                                  ttattaCTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGaa                                      >  1:806274/1‑100 (MQ=255)                                  gcattaCTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGaa                                      >  1:935071/3‑100 (MQ=255)                                    attaCTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGgagatcg                                    >  1:1753449/1‑97 (MQ=255)                                    attaCTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGaaaa                                    >  1:1035624/1‑100 (MQ=255)                                    attaCTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGaaaa                                    >  1:1483674/1‑100 (MQ=255)                                    attaCTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGaaaa                                    >  1:3609399/1‑100 (MQ=255)                                    attaCTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGaaaa                                    >  1:1676748/1‑100 (MQ=255)                                    attaCTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGaaaa                                    >  1:2149219/1‑100 (MQ=255)                                    attaCTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGACTAAAATCAGGAGaaaa                                    >  1:3495815/1‑100 (MQ=255)                                    attaCTTTATACAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATCTTCAAAGTCGATTAAAATCAGGAGaaaa                                    >  1:1990541/1‑100 (MQ=255)                                    attaCTTTATACAAGCACCAATGCAAAAAACGATGATCCATGGCTTTGAAGCTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGaaaa                                    >  1:3297930/1‑100 (MQ=255)                                           tataCAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAAGCAGgagatcggaagagc                             >  1:1806251/1‑90 (MQ=255)                                           gctaCAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGaaa                             >  1:2938937/3‑100 (MQ=255)                                            ataCAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAat                            >  1:750960/1‑100 (MQ=255)                                            ataCAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAat                            >  1:5716840/1‑100 (MQ=255)                                             taCAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAata                           >  1:5380921/1‑100 (MQ=255)                                              ccAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAatat                          >  1:1775334/2‑100 (MQ=255)                                              aCAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAatat                          >  1:5341627/1‑100 (MQ=255)                                              aCAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAatat                          >  1:2982615/1‑100 (MQ=255)                                               cAAGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAAtata                         >  1:108915/1‑100 (MQ=255)                                                tgtgcccAATGC‑AAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACt                       >  1:3693539/6‑100 (MQ=255)                                                acgCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATAc                        >  1:4822953/3‑100 (MQ=255)                                                aaGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATAc                        >  1:4529441/1‑100 (MQ=255)                                                aaGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATAc                        >  1:3332802/1‑100 (MQ=255)                                                aaGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATAc                        >  1:5109130/1‑100 (MQ=255)                                                 aGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACt                       >  1:1134743/1‑100 (MQ=255)                                                 aGCACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACt                       >  1:3605360/1‑100 (MQ=255)                                                   cACCAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTaa                     >  1:1343888/1‑100 (MQ=255)                                                   agccAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTaa                     >  1:397183/3‑100 (MQ=255)                                                     ccAATGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTAAAt                   >  1:2004566/1‑100 (MQ=255)                                                       aaTGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTAAATGa                 >  1:1671173/1‑100 (MQ=255)                                                       aaTGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATCTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTAAATGa                 >  1:2800026/1‑100 (MQ=255)                                                        aTGCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTAAATGat                >  1:1841410/1‑100 (MQ=255)                                                          gCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATGTACTAAATGatat              >  1:5487801/1‑100 (MQ=255)                                                          gCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTAAATGatat              >  1:621224/1‑100 (MQ=255)                                                          gCAAAAAATGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTAAATGatat              >  1:2218150/1‑100 (MQ=255)                                                               aaaTGATGATCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTAAATGATATCGGGt         >  1:4533668/1‑100 (MQ=255)                                                                      gatCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTAAATGATATTGGGTGATATgg  >  1:1354328/1‑100 (MQ=255)                                                                      gatCCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTAAATGATATTGGGTGATATgg  >  1:299764/1‑100 (MQ=255)                                                                         |                                                                                                 ATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATTCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGAGAAAATATGAAATATACTAAATGATATTGGGTGATATGG  >  NC_007793/718849‑719017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑