Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	705,053	A→G	intergenic (+150/‑209)	SAUSA300_RS03375 → / → SAUSA300_RS03380	antibiotic ABC transporter ATP‑binding protein/nucleoside permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	705,053	0	A	G	100.0%	45.4 / NA	18	intergenic (+150/‑209)	SAUSA300_RS03375/SAUSA300_RS03380	antibiotic ABC transporter ATP‑binding protein/nucleoside permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/2);  total (16/2)

TGTGGCACATTGATGGATATAGATGTTAATAAATTGCTTCAAGCTTTTGTCTATTTTAAATCATTTGAGAAGTTACGACATAATAATTCTTAAATTAATGAAATCGATATTTTAAGAAAAAAATGCTCATGGTATAATACAAGTTATAAGCAAACATACATATATTAAATACTGTAGCCACG  >  NC_007793/704962‑705143                                                                                            |                                                                                           tgtgGCACATTGATGGATATAGATGTTAATAAATTGCTTCAAGCTTTTGTCTATTTTAAATCATTTGAGAAGTTACGACATAATAATTCTTGAATTAATg                                                                                    >  1:3602558/1‑100 (MQ=255)   tgGCACATTGATGGATATAGATGTTAATAAATTGCTTCAAGCTTTTGTCTATTTTAAATCATTTGAGAAGTTACGACATAATAATTCTTGAATTAATGaa                                                                                  >  1:750004/1‑100 (MQ=255)    ggCACATTGATGGATATAGATGTTAATAAATTGCTTCAAGCTTTTGTCTATTTTAAATCATTTGAGAAGTTACGACATAATAATTCTTGAATTAATGaaa                                                                                 >  1:813269/1‑100 (MQ=255)              tGGATATAGATGTTAATAAATTGCTTCAAGCTTTTGTCTATTTTAAATCATTTGAGAAGTTACGACATAATAATTCTTGAATTAATGAAATCGATAtttt                                                                       >  1:3960213/1‑100 (MQ=255)                     aGATGTTAATAAATTGCTTCAAGCTTTTGTCTATTTTAAATCATTTGAGAAGTTACGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAGaaaa                                                                >  1:1745051/1‑100 (MQ=255)                                   tGCTTCAAGCTTTTGTCTATTTTAAATCATTTGAGAAGTTACGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAGAAAAAAATGCCCATggag                                                  >  1:277060/1‑98 (MQ=255)                                       tCAAGCTTTTGTCTATTTTAAATCATTTGAGAAGTTACGACATAATAATTCTTGAATTAGTGAAATCGATATTTTAAGAAAAAAATGCTCATGGTataat                                              >  1:2694634/1‑100 (MQ=255)                                        cAAGCTTTTGACTATTTTAAATCATTTGAGAAGTTACGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAGAAAAAAATACTCATGGTataata                                             >  1:3282318/1‑100 (MQ=255)                                                   cTATTTTAAATCATTTGAGAAGTTACGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAG‑AAAAAATGCTCATGGTATAATACAAGTTATAAGc                                 >  1:2106635/1‑100 (MQ=255)                                                                          tACGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAGAAAAAAATGCTCATGGTATAATACAAGTTATAAGCAAACATACATATATTAAATACt           >  1:3732548/1‑100 (MQ=255)                                                                          tACGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAGAAAAAAATGCTCATGGTATAATACAAGTTATAAGCAAACATACATATATTAAATACt           >  1:1721348/1‑100 (MQ=255)                                                                           aCGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAGAAAAAAATGCTCATGGTATAATACAAGTTATAAGCAAACATACATATATTAAATACTg          >  1:382543/1‑100 (MQ=255)                                                                           aCGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAGAAAAAAATGCTCATGGTATAATACAAGTTATAAGCAAACATACATATATTAAATACTg          >  1:4191176/1‑100 (MQ=255)                                                                           aCGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAGAAAAAAATGCTCATGGTATAATACAAGTTATAAGCAAACATACATATATTAAATACTg          >  1:639557/1‑100 (MQ=255)                                                                           aCGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAGAAAAAAATGCTCATGGTATAATACAAGTTATAAGCAAACATACATATATTAAATACTg          >  1:2457007/1‑100 (MQ=255)                                                                           aCGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAGAAAAAAATGCTCATGGTATAATACAAGTTATAAGCAAACATACATATATTAAATACTg          >  1:988224/1‑100 (MQ=255)                                                                            cGACATAATAATTCTTGAATTAATGAAATCGATATTTTAAGAAAAAAATGCTCATGGTATAATACAAGTTATAAGCAAACATACATATATTAAATACTGt         <  1:649478/100‑1 (MQ=255)                                                                                   ataatTCTTGAATTAATGAAATCGATATTTTAAGAAAAAAATGCTCATGGTATAATACAAGTTATAAGCAAACATACATATATTAAATACTGTAGCCACg  <  1:3465182/100‑1 (MQ=255)                                                                                            |                                                                                           TGTGGCACATTGATGGATATAGATGTTAATAAATTGCTTCAAGCTTTTGTCTATTTTAAATCATTTGAGAAGTTACGACATAATAATTCTTAAATTAATGAAATCGATATTTTAAGAAAAAAATGCTCATGGTATAATACAAGTTATAAGCAAACATACATATATTAAATACTGTAGCCACG  >  NC_007793/704962‑705143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑