Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	700,471	A→T	E217D (GAA→GAT)	SAUSA300_RS03360 →	glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	700,471	0	A	T	100.0%	246.5 / NA	83	E217D (GAA→GAT)	SAUSA300_RS03360	glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (82/1);  total (82/1)

TACTTGTCAAGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGAAGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCTATTATATAGTG  >  NC_007793/700374‑700565                                                                                                  |                                                                                               gaCTTGTCAAGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACgatga                                                                                              >  1:3077089/2‑100 (MQ=255)  aCTTGTCAAGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACgatgaa                                                                                             >  1:2435946/1‑100 (MQ=255)   cTTGTCAAGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGacgaag                                                                                            >  1:2658651/1‑100 (MQ=255)      cacAAGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGAtc                                                                                         >  1:1993673/3‑99 (MQ=255)       ctaaGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGAttt                                                                                        >  1:795037/3‑100 (MQ=255)       ccAAGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGAtcg                                                                                        >  1:4978357/2‑98 (MQ=255)       aaaaGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGAttt                                                                                        >  1:4498994/3‑100 (MQ=255)        cAAGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGAtttt                                                                                       >  1:924207/1‑100 (MQ=255)        cAAGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGAtcgg                                                                                       >  1:4832836/1‑97 (MQ=255)         taGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTa                                                                                      >  1:620871/2‑100 (MQ=255)         gtgtggATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTa                                                                                      >  1:3103807/6‑100 (MQ=255)         ctggCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTa                                                                                      >  1:5188180/3‑100 (MQ=255)              taTGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGAtcggaagagc                                                                                 >  1:538173/2‑91 (MQ=255)               aTGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAATGATGAAGAtcggaagagca                                                                                >  1:4664325/1‑90 (MQ=255)               aTGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAGGAtcggaagagca                                                                                >  1:5058160/1‑90 (MQ=255)               aTGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGat                                                                                >  1:2919245/1‑100 (MQ=255)               aTGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACAATGAAGAtcggaagagca                                                                                >  1:1515701/1‑90 (MQ=255)                 tgaaaaaGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGatat                                                                              >  1:3078343/3‑100 (MQ=255)                 gCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGatat                                                                              >  1:4917595/1‑100 (MQ=255)                 gCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGatat                                                                              >  1:2440050/1‑100 (MQ=255)                 gCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGatat                                                                              >  1:1047508/1‑100 (MQ=255)                 gCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGatat                                                                              >  1:5141598/1‑100 (MQ=255)                  tataaaGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGatata                                                                             >  1:3833342/4‑100 (MQ=255)                    taaaGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATc                                                                           >  1:4472994/2‑100 (MQ=255)                      aaGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATcac                                                                         >  1:1124282/1‑100 (MQ=255)                           aCGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGaaa                                                                    >  1:2853530/1‑100 (MQ=255)                                 ataCATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACACGTAACGATGAAGATTTTAATAGATATATCCCAGAAATTATGa                                                              >  1:5054207/1‑100 (MQ=255)                                 ataCATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGa                                                              >  1:4595627/1‑100 (MQ=255)                                 ataCATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGa                                                              >  1:1628336/1‑100 (MQ=255)                                  taCATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGaa                                                             >  1:5200705/1‑100 (MQ=255)                                           cttCACAGATGATTGTCGGAGATAACGATATAGTTACACATGCTACAAGTAACGATGAAGGTTTTAATAGATATATCACAGAAATTATGAAAATAAGACa                                                    >  1:1072688/2‑100 (MQ=255)                                            ttCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACaa                                                   >  1:4096934/1‑100 (MQ=255)                                             tCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGCAACGATGAAGATTTTACTAGATATATCACAGAAATTATGAAAATAAGACAAc                                                  >  1:2182930/1‑100 (MQ=255)                                              cacaGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACagca                                                 >  1:2861351/1‑97 (MQ=255)                                              cacaGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACg                                                 >  1:669004/1‑100 (MQ=255)                                              agcaGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACg                                                 >  1:4616971/3‑100 (MQ=255)                                                   aagatTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCa                                            >  1:2133741/3‑100 (MQ=255)                                                    tgatTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCAt                                           >  1:3443900/1‑100 (MQ=255)                                                    agatTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAgatcg                                           >  1:215584/2‑96 (MQ=255)                                                     gatTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAgatcgg                                          >  1:5234801/1‑95 (MQ=255)                                                     gatTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAgatcgg                                          >  1:2041756/1‑95 (MQ=255)                                                     gatTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATg                                          >  1:1544776/1‑100 (MQ=255)                                                        tGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGaa                                       >  1:3679459/1‑100 (MQ=255)                                                           cGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATg                                    >  1:4986832/1‑100 (MQ=255)                                                           cGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATg                                    >  1:3348800/1‑100 (MQ=255)                                                             gagaTAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGtt                                  >  1:751521/1‑100 (MQ=255)                                                                          aGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTgca                     >  1:2465761/1‑98 (MQ=255)                                                                          aGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGaa                     >  1:5287232/1‑100 (MQ=255)                                                                          aGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGaa                     >  1:143097/1‑100 (MQ=255)                                                                          aGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGaa                     >  1:1742886/1‑100 (MQ=255)                                                                          aGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGaa                     >  1:2273483/1‑100 (MQ=255)                                                                          aGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGaa                     >  1:4824641/1‑100 (MQ=255)                                                                          aGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGaa                     >  1:3466516/1‑100 (MQ=255)                                                                          aGATACACATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATCAAAATAAGACAACGAGCCATGGAAATGTTACTATTACCTGaa                     >  1:5366203/1‑100 (MQ=255)                                                                            agacacATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACa                   >  1:601621/3‑100 (MQ=255)                                                                              tcacaTGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACaaa                 >  1:2141379/2‑100 (MQ=255)                                                                              acacATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACaaa                 >  1:852422/1‑100 (MQ=255)                                                                              acacATGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACaaa                 >  1:3760562/1‑100 (MQ=255)                                                                               tccaTGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAg                >  1:1829140/3‑100 (MQ=255)                                                                                gcaTGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAgg               >  1:5633065/2‑100 (MQ=255)                                                                                acttGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAgg               >  1:5766853/4‑100 (MQ=255)                                                                                acaTGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAgg               >  1:1976041/1‑100 (MQ=255)                                                                                acaTGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAgg               >  1:4163906/1‑100 (MQ=255)                                                                                 caTGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGc              >  1:3559802/1‑100 (MQ=255)                                                                                 caTGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGc              >  1:3246123/1‑100 (MQ=255)                                                                                  ctGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCt             >  1:533819/2‑100 (MQ=255)                                                                                  aTGCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGca             >  1:4946028/1‑99 (MQ=255)                                                                                    gCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtat           >  1:995445/1‑100 (MQ=255)                                                                                    gCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtat           >  1:478133/1‑100 (MQ=255)                                                                                    gCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtat           >  1:2641829/1‑100 (MQ=255)                                                                                    gCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtat           >  1:3112091/1‑100 (MQ=255)                                                                                    gCTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtat           >  1:2980459/1‑100 (MQ=255)                                                                                     cTACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtatt          >  1:4289166/1‑100 (MQ=255)                                                                                      tACAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtatta         >  1:5608978/1‑100 (MQ=255)                                                                                       aCAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtattat        >  1:939807/1‑100 (MQ=255)                                                                                       aCAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtattat        >  1:4974840/1‑100 (MQ=255)                                                                                       aCAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtattat        >  1:2083087/1‑100 (MQ=255)                                                                                       aCAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtattat        >  1:2261719/1‑100 (MQ=255)                                                                                       aCAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtattat        >  1:3613931/1‑100 (MQ=255)                                                                                       aCAAGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCtattat        >  1:324632/1‑100 (MQ=255)                                                                                         caGTAACGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCTATtatat      >  1:1765586/2‑100 (MQ=255)                                                                                            caaCGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCTATTATATAGt   >  1:5737160/2‑100 (MQ=255)                                                                                             aaCGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCTATTATATAGTg  >  1:4035054/1‑100 (MQ=255)                                                                                             aaCGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCTATTATATAGTg  <  1:4848907/100‑1 (MQ=255)                                                                                             aaCGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCTATTATATAGTg  >  1:5337750/1‑100 (MQ=255)                                                                                             aaCGATGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCTATTATATAGTg  >  1:5223905/1‑100 (MQ=255)                                                                                                  |                                                                                               TACTTGTCAAGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCACAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGAAGAAGATTTTAATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTACCTGAACAAAGGCTATTATATAGTG  >  NC_007793/700374‑700565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑