Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	683,400	C→G	Q423E (CAA→GAA)	SAUSA300_RS03275 →	cation:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	683,400	0	C	G	100.0%	25.5 / NA	12	Q423E (CAA→GAA)	SAUSA300_RS03275	cation:proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (7/5);  total (7/5)

CTATGGCTGGAGTACCATTTTTAAATGGATTTTTAAGTAAAGAAATG‑TTTTTAGATGCATTAACACAAACTGGACAATTATCCCAATTTAGTTTGATTTCAATGATAGCTATCGTGTTTGTTGGTGTTATTGCGAGTGTTTTTACATTCACATATGCACTATACATGGTAAA  >  NC_007793/683317‑683488                                                                                     |                                                                                         gtatGGCTGGAGTACCATTTTTAAATGGATTTTTAAGTAAAGAAATG‑TTTTTAGATGCATTAACACAAACTGGACAATTATCCGAATTTAGTTTGATTTc                                                                          >  1:1159512/2‑100 (MQ=255) gtatGGCTGGAGTACCATTTTTAAATGGATTTTTAAGTAAAGAAATG‑TTTTTAGATGCATTAACACAAACTGGACAATTATCCGAATTTAGTTTGATTTc                                                                          >  1:1164403/2‑100 (MQ=255) ctatGGCTGGAGTACCATTTTTAAATGGATTTTTAAGTAAAGAAATG‑TTTTTAGATGCATTAACACAAACTGGACAATTATCCGAATTTAGTTTGATTTc                                                                          >  1:5277676/1‑100 (MQ=255)  catGGCTGGAGTACCATTTTTAAATGGATTTTTAAGTAAAGAAATG‑TTTTTAGATGCATTAACACAAACTGGACAATTATCCGAATTTAGTTTGATTTCa                                                                         >  1:288190/2‑100 (MQ=255)   atGGCTGGAGTACCATTTTTAAATGGATTTTTAAGTAAAGAAATGTTTTTTAGATGCATTAACACAAACTGGACAATTATCCGAATTTAGTTTGATTTCa                                                                         >  1:3752370/1‑100 (MQ=255)   atGGCTGGAGTACCATTTTTAAATGGATTTTTAAGTAAAGAAATG‑TTTTTAGATGCATTAACACAAACTGGACAATTATCCGAATTTAGTTTGATTTCaa                                                                        >  1:559160/1‑100 (MQ=255)   atGGCTGGAGTACCATTTTTAAATGGATTTTTAAGTAAAGAAATG‑TTTTTAGATGCATTAACACAAACTGGACAAGTATCCGAATTTAGTTGGAGTTCaa                                                                        >  1:1326940/1‑100 (MQ=255)                      tAAATGGATTTTTAAGTAAAGAAATG‑TTTTTAGATGCATTAACACAAACTGGACAATTATCCGAATTTAGTTTGATTTCAATGATAGCTATCGtgtttga                                                     <  1:2549258/100‑2 (MQ=255)                      tAAATGGATTTTTAAGTAAAGAAATG‑TTTTTAGATGCATTAACACAAACTGGACAATTATCCGAATTTAGTTTGATTTCAATGATAGCTATCGtgtttga                                                     <  1:4214731/100‑2 (MQ=255)                                         aGAAATG‑TTTTTAGATGCATTAACACAAACTGGACAATTATCCGAATTTAGTTTGATTTCAATGATAGCTATCGTGTTTGTTGGTGTTATTGCGatgtgc                                  <  1:5647573/100‑6 (MQ=255)                                                                acacAAACTGGACAATTATCCGAATTTAGTTTGATTTCAATGATAGCTATCGTGTTTGTTGGTGTTATTGCGAGTGTTTTTACATTCACATATGCACtat            <  1:32008/100‑1 (MQ=255)                                                                          gACAATTATCCGAATTTAGTTTGATTTCAATGATAGCTATCGTGTTTGTTGGTGTTATTGCGAGTGTTTTTACATTCACATATGCACTATACATGGTaaa  <  1:2291798/100‑1 (MQ=255)                                                                                     |                                                                                         CTATGGCTGGAGTACCATTTTTAAATGGATTTTTAAGTAAAGAAATG‑TTTTTAGATGCATTAACACAAACTGGACAATTATCCCAATTTAGTTTGATTTCAATGATAGCTATCGTGTTTGTTGGTGTTATTGCGAGTGTTTTTACATTCACATATGCACTATACATGGTAAA  >  NC_007793/683317‑683488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑