Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	659,200	A→G	intergenic (+89/‑364)	SAUSA300_RS03135 → / → SAUSA300_RS03140	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	659,200	0	A	G	100.0%	17.4 / NA	8	intergenic (+89/‑364)	SAUSA300_RS03135/SAUSA300_RS03140	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/2);  total (6/2)

AGTAGTAAAAATGATCGAAATTGGTAGTATCGCATATTTAAACGGTGGTTCAAAAAAATATAATCATATCTTAAATCAGGAAAATAGATAGTAATGTTTGAATATACCAAGTGAAAATGCCCATTGTGATTGATAAAGGAAAAAACTGTTTTAATTTAAAAATGAAATATAGAGCGTGTTATT  >  NC_007793/659107‑659289                                                                                              |                                                                                          agtagtAAAAATGATCGAAATTGGTAGTATCGCATATTTAAACGGTGGTTCAAAAAAATATAATCATATCTTAAATCAGGAAAATAGATAGTAGTGTTTg                                                                                     <  1:5536307/100‑1 (MQ=255)                            gaTCGCATATTTAAACGGTGGTTCAAAAAAATATAATCATATCTTAAATCAGGAAAATAGATAGTAGTGTTTGAATATACCAAGTGAAAATGCCCATtgt                                                          >  1:2933988/2‑100 (MQ=255)                             attgCATATTTAAACGGTGGTTCAAAAAAATATAATCATATCTTAAATCAGGAAAATAGATAGTAGTGTTTGAATATACCAAGTGAAAATGCCCagatcg                                                         >  1:3327985/4‑95 (MQ=255)                                           tggtggtTCAAAAAAATATAATCATATCTTAAATCAGGAAAATAGATAGTAGTGTTTGAATATACCAAGTGAAAATGCCCATTGTGATTGATAAAGGaaa                                           >  1:5762413/2‑100 (MQ=255)                                                                     gtgtAAATCAGGAAAATAGATAGTAGTGTTTGAATATACCAAGTGAAAATGCCCATTGTGATTGATAAAGGAAAAAACTGTTTTAATTTAAAAATGAAat                 >  1:764581/4‑100 (MQ=255)                                                                        tAAATCAGGAAAATAGATAGTAGTGTTTGAATATACCAAGTGAAAATGCCCATTGTGATTGATAAAGGAAAAAACTGTTTTAATTTAAAAATGAAAtata              >  1:5670725/1‑100 (MQ=255)                                                                         aaaTCAGGAAAATAGATAGTAGTGTTTGAATATACCAAGTGAAAATGCCCATTGTGATTGATAAAGGAAAAAACTGTTTTAATTTAAAAATGAAATATag             <  1:3828114/100‑1 (MQ=255)                                                                                    atagatagTAGTGTTTGAATATACCAAGTGAAAATGCCCATTGTGATTGATAAAGGAAAAAACTGTTTTAATTTAAAAATGAAATATAGAGCGTGTTAtt  >  1:2365923/1‑100 (MQ=255)                                                                                              |                                                                                          AGTAGTAAAAATGATCGAAATTGGTAGTATCGCATATTTAAACGGTGGTTCAAAAAAATATAATCATATCTTAAATCAGGAAAATAGATAGTAATGTTTGAATATACCAAGTGAAAATGCCCATTGTGATTGATAAAGGAAAAAACTGTTTTAATTTAAAAATGAAATATAGAGCGTGTTATT  >  NC_007793/659107‑659289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑