Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	657,078	C→A	P12P (CCC→CCA)	SAUSA300_RS03125 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	657,078	0	C	A	100.0%	58.1 / NA	23	P12P (CCC→CCA)	SAUSA300_RS03125	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/0);  total (23/0)

TATGTTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCC  >  NC_007793/656987‑657175                                                                                            |                                                                                                  tatGTTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAg                                                                                           >  1:4114429/1‑100 (MQ=255)    attAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAat                                                                                        >  1:1875456/2‑100 (MQ=255)      tAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAatta                                                                                      >  1:4156671/1‑100 (MQ=255)      tAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAatta                                                                                      >  1:4139234/1‑100 (MQ=255)      tAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAatta                                                                                      >  1:3815037/1‑100 (MQ=255)      tAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAatta                                                                                      >  1:2294226/1‑100 (MQ=255)      tAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAatta                                                                                      >  1:2801853/1‑100 (MQ=255)       aGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAattat                                                                                     >  1:1398653/1‑100 (MQ=255)       aGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAattat                                                                                     >  1:5584774/1‑100 (MQ=255)       aGCCACGCTTCAAATAAAAAATAATTAGAATAAGGGGGGATTGATAAGCAATGCTATGCGAATTTAAAATCATCAATAAAAACGCAAAATATAGAattat                                                                                     >  1:833449/1‑100 (MQ=21)             ccTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAATTATTAAata                                                                               >  1:1017500/2‑100 (MQ=255)                                 aGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGa                                                           >  1:4566687/1‑100 (MQ=255)                                   taTAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATc                                                         >  1:3704719/2‑100 (MQ=255)                                      aaGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGat                                                      >  1:2069548/1‑100 (MQ=255)                                          tGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATataa                                                  >  1:4897142/1‑100 (MQ=255)                                                       gaaTGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGAt                                     >  1:639517/2‑100 (MQ=25)                                                       cAATGCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGAt                                     >  1:5373763/1‑100 (MQ=25)                                                           gCTATGCGAATCTAAAATCATCAATAAAAACCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGt                                 >  1:2213627/1‑100 (MQ=17)                                                                                caATAAAAACCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAAt            >  1:1935212/1‑100 (MQ=17)                                                                                      aaaCCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTc      >  1:1845612/1‑100 (MQ=17)                                                                                       aaCCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCa     >  1:3328688/1‑100 (MQ=17)                                                                                       aaCCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCa     >  1:4356200/1‑100 (MQ=17)                                                                                        aCCCAAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCAt    >  1:243915/1‑100 (MQ=17)                                                                                          cccAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTGTTTTTTCCTTTTATTAATTGGTTCATcc  >  1:1709881/1‑100 (MQ=255)                                                                                            |                                                                                                  TATGTTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCC  >  NC_007793/656987‑657175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑