Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	656,348	G→T	S44I (AGT→ATT)	SAUSA300_RS03115 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	656,348	0	G	T	100.0%	50.4 / NA	20	S44I (AGT→ATT)	SAUSA300_RS03115	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/14);  total (6/14)

TATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGAATAGTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGC  >  NC_007793/656257‑656438                                                                                            |                                                                                           tataGAATTATTAAATACAGTAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCaaaa                                                                                    <  1:5044518/100‑1 (MQ=17)                      aatGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTagag                                                               <  1:514361/100‑1 (MQ=255)                                 cATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAgaagagaagatcggaag                                                    >  1:5481655/1‑93 (MQ=17)                                             agtaagtaCTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTCAAagatc                                        >  1:5288101/1‑99 (MQ=21)                                             agtaagtaCTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGCACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATAtt                                        >  1:4241892/1‑100 (MQ=21)                                                aagtaCTTGGATTGCTTATTTTTTGCCCATGGTTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTc                                     <  1:2285672/100‑1 (MQ=25)                                                 agtaCTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCa                                    <  1:3835638/100‑1 (MQ=255)                                                 agtaCTTGGATTGCTTA‑TTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCac                                   <  1:458771/100‑2 (MQ=21)                                                  gtaCTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCaa                                   <  1:293232/100‑1 (MQ=255)                                                       tGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCg                              <  1:2006569/100‑1 (MQ=255)                                                                tATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAaata                     >  1:4877716/1‑100 (MQ=255)                                                                       tGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACttt              >  1:2015108/1‑100 (MQ=255)                                                                        gCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTc             >  1:4296571/1‑100 (MQ=255)                                                                              gATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCt       <  1:2235716/100‑1 (MQ=255)                                                                               aTTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCta      <  1:2430232/100‑2 (MQ=255)                                                                                ttAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGt     <  1:2128919/100‑1 (MQ=255)                                                                                  aaTTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTg   <  1:3991730/100‑1 (MQ=255)                                                                                   aTTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGc  <  1:3018277/100‑1 (MQ=255)                                                                                   aTTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGc  <  1:2454011/100‑1 (MQ=255)                                                                                   aTTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGACAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGc  <  1:681961/100‑1 (MQ=21)                                                                                            |                                                                                           TATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGAATAGTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGC  >  NC_007793/656257‑656438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑