Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	652,201	T→C	intergenic (+35/‑431)	SAUSA300_RS03090 → / → SAUSA300_RS03095	hypothetical protein/transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	652,201	0	T	C	100.0%	24.7 / NA	11	intergenic (+35/‑431)	SAUSA300_RS03090/SAUSA300_RS03095	hypothetical protein/transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/0);  total (11/0)

TGATGCATTAACGGTGAGATTGCACAATTGAAATTTTAATTATGTATGAACTGATAGATATGAAATGAATATTGAAAATGAGAGCAATAAAATTTTGAATGATGATGTTGTGTGATTGTATAAACTTATTCGTATTTTAGATACGTTTTGATATTTCGC  >  NC_007793/652136‑652294                                                                  |                                                                                              cgATGCATTAACGGTGAGATTGCACAATTGAAATTTTAATTATGTATGAACTGATAGATATGAAACGAATATTGAAAATGAGAGCAATAAAATTTTGAat                                                             >  1:5295787/2‑100 (MQ=255)  gATGCATTAACGGTGAGATTGCACAATTGAAATTTTAATTATGTATGAACTGATAGATATGAAACGAATATTGACAATGAGAGCAATAAAATTTTGAatg                                                            >  1:5796350/1‑100 (MQ=255)   aTGCATTAACGGTGAGATTGCACAATTGAAATTTTAATTATGTATGAACTGATAGATATGAAACGAATATTGAAAATGAGAGCAATAAAATTTTGAatga                                                           >  1:2115489/1‑100 (MQ=255)             tgTGAGATTGCACAATTGAAATTTTAATTATGTATGAACTGATAGATATGAAACGAATATTGAAAATGAGAGCAATAAAATTTTGAATGATGATGTtgtg                                                 >  1:5220736/2‑100 (MQ=255)                gagaTTGCACAATTGAAATTTTAATTATGTATGAACTGATAGATATGAAGCGAATATTGAAAATGAGAGCAATAAAATTTTGAATGATGATGTTGTGTGa                                              >  1:1086262/1‑100 (MQ=255)                    aggCACAATTGAAATTTTAATTATGTATGAACTGATAGATATGAAACGAATATTGAAAATGAGAGCAATAAAATTTTGAATGATGATGTTGTGTGATTGt                                          >  1:539398/3‑100 (MQ=255)                     tGCACAATTGAAATTTTAATTATGTATGAACTGATAGATATGAAACGAATATTGAAAATGAGAGCAATAAAATTTTGAATGATGATGTTGTGTGATTGta                                         >  1:5083413/1‑100 (MQ=255)                      gtacaATTGAAATTTTAATTATGTATGAACTGATAGATATGAAACGAATATTGAAAATGAGAGCAATAAAATTTTGAATGATGATGTTGTGTGATTGtat                                        >  1:1420350/3‑100 (MQ=255)                                                     gatagataTGAAACGAATATTGAAAATGAGAGCAATAAAATTTTGAATGATGATGTTGTGTGATTGTATAAACTTATTCGTATTTTAGATACGTTTTGat         >  1:4326999/1‑100 (MQ=255)                                                     gatagataTGAAACGAATATTGAAAATGAGAGCAATAAAATTTTGAATGATGATGTTGTGTGATTGTATAAACTTATTCGTATTTTAGATACGTTTTGat         >  1:5038682/1‑100 (MQ=255)                                                     gatagataTGAAACGAATATTGAAAATGAGAGCAATAAAATTTCGAATGATGATGTTGTGTGATTGTATAAACTTATTCGTATTTTAGATACGTTTTGat         >  1:826922/1‑100 (MQ=255)                                                            atgaaacgaaTATTGAAAATGAGAGCAATAAAATTTTGAATGATGATGTTGTGTGATTGTATAAACTTATTCGTATTTTAGATACGTTTTGATATTTCGc  <  1:4636625/100‑1 (MQ=255)                                                                  |                                                                                              TGATGCATTAACGGTGAGATTGCACAATTGAAATTTTAATTATGTATGAACTGATAGATATGAAATGAATATTGAAAATGAGAGCAATAAAATTTTGAATGATGATGTTGTGTGATTGTATAAACTTATTCGTATTTTAGATACGTTTTGATATTTCGC  >  NC_007793/652136‑652294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑