Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 650,151 T→A V96E (GTA→GAA)  SAUSA300_RS03075 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793650,1510TA97.3% 303.2 / ‑2.4 113V96E (GTA→GAA) SAUSA300_RS03075hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base A (103/6);  total (107/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 113 total aligned reads displayed.

TCGAAAAAGTTTCTCAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGTAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAGAAAAGCGAAAAGCACAACAATAAAGCCTGATGGCA  >  NC_007793/650058‑650218
                                                                                             |                                                                      
tCGAAAAAGTTTCTCAACAATTAAAGGAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑Ca                                                                 <  1:2716102/100‑1 (MQ=255)
tCGAAAAAGTTTCTCAACAATTAAAGAAACCACGTGATCCGGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑cg                                                                 <  1:1258243/100‑2 (MQ=255)
    aaaaGTTTCTCAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGAt                                                             <  1:4621419/100‑1 (MQ=255)
           tctcAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGc                                                      >  1:5165519/1‑100 (MQ=255)
           cctcAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGc                                                      >  1:2343530/2‑100 (MQ=255)
            ctcAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCt                                                     >  1:2998841/1‑100 (MQ=255)
              taacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTa                                                   >  1:4189918/2‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTACACGTCGTAAAGTAGAA‑CAAGATAGAAAGCTTa                                                   >  1:5181068/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAGGAA‑CAAGATCGGAAgagca                                                   >  1:1725104/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAAGCAAGATCGGAAgagc                                                    >  1:4310939/1‑97 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTa                                                   >  1:2348611/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTa                                                   >  1:2312415/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTa                                                   >  1:4381389/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTa                                                   >  1:2555970/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTa                                                   >  1:1855614/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTa                                                   >  1:492544/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTa                                                   >  1:372595/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTa                                                   >  1:5523261/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTa                                                   >  1:71496/1‑100 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAG‑A‑CAAGATCGGAAgagcac                                                  >  1:1392317/1‑95 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAATA‑CAAGATCGGAAgagca                                                   >  1:1713764/1‑96 (MQ=255)
              caacaaTTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAAAA‑CAAGATCGGAAgagca                                                   >  1:1364747/1‑96 (MQ=255)
              caacaaTTAAAGAAACCAAGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATAAAGAACGTAAACGTCGTAAAGTAGAA‑CAAGATAGAAAGCTTa                                                   >  1:2623875/1‑100 (MQ=255)
                      aaaGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑aaa‑aa                                         >  1:5531841/1‑100 (MQ=255)
                         gAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTa                                      >  1:2440533/1‑100 (MQ=255)
                         gAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AACTa                                      >  1:19269/1‑100 (MQ=255)
                          ataCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACAACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTac                                     >  1:4774430/3‑99 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGGAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AAGTAg                                     >  1:916500/1‑100 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAg                                     >  1:1973936/1‑100 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAg                                     >  1:1097485/1‑100 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAg                                     >  1:1478863/1‑100 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAg                                     >  1:741593/1‑100 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATCAg                                     >  1:1922886/1‑100 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATAAg                                     >  1:4806290/1‑100 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AACTAg                                     >  1:4071289/1‑100 (MQ=255)
                          aaaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGACGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAg                                     >  1:5010254/1‑100 (MQ=255)
                           taCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGATGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATCAga                                    >  1:3755701/2‑100 (MQ=255)
                           taCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAga                                    >  1:3886749/2‑100 (MQ=255)
                           aaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAG‑AATTAga                                    >  1:3009285/1‑100 (MQ=255)
                           aaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgt                                    >  1:4264574/1‑99 (MQ=255)
                           aaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAga                                    <  1:5283817/100‑1 (MQ=255)
                           aaCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGAAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTata                                    >  1:4320620/1‑98 (MQ=255)
                            tccACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaa                                   >  1:2455392/2‑100 (MQ=255)
                            gccACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AACCAgaa                                   >  1:3780886/2‑100 (MQ=255)
                            cccACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaa                                   >  1:5206633/2‑100 (MQ=255)
                            aCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAaaa‑aaa‑aaaacgaa                                   >  1:4012553/1‑94 (MQ=255)
                            aCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AGA‑AATTAgat                                   >  1:1171027/1‑99 (MQ=255)
                            aCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATCAgaa                                   >  1:4801790/1‑100 (MQ=255)
                            aCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AAATAgaa                                   >  1:2080060/1‑100 (MQ=255)
                            aCCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAA‑AAA‑AATAAgaa                                   >  1:2697827/1‑100 (MQ=255)
                             gcACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAaaa‑aaa‑aaaaaaaaa                                  >  1:2233051/2‑93 (MQ=255)
                             ccACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑aaa‑aaatgagaa                                  >  1:868701/1‑93 (MQ=255)
                             ccACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATCAgaaa                                  <  1:5058350/100‑1 (MQ=255)
                              cACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaa                                 >  1:3116383/1‑100 (MQ=255)
                              cACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AAATAgaaag                                 >  1:3306160/1‑99 (MQ=255)
                              cACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AAATAgaaag                                 >  1:1226605/1‑99 (MQ=255)
                               gcGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaag                                >  1:4495464/2‑100 (MQ=255)
                               aCGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgacaag                                >  1:1914419/1‑100 (MQ=255)
                                cGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAGAAAA‑AATGAgaaaag                                >  1:1139321/1‑100 (MQ=255)
                                 gTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagcg                              >  1:2388925/1‑100 (MQ=255)
                                 gTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagat                              >  1:4837202/1‑98 (MQ=255)
                                  tGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACGTGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAtaagatcgg                             >  1:128795/1‑94 (MQ=255)
                                  tGACCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATCAgaaaagatc                             >  1:2360884/1‑97 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAAGGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAggaaagatcg                            >  1:4496334/1‑96 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAG‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagatcg                            >  1:4157245/1‑96 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAGAAAA‑AATAAgaaaagatc                             >  1:4142920/1‑97 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑Aaaaagaaaagatcg                            >  1:5116021/1‑96 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTacagatcggag                            >  1:4088231/1‑90 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaagatcgga                            >  1:2692171/1‑93 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaagatcgg                            >  1:2930418/1‑94 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagatcg                            >  1:1566216/1‑96 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAagaaagatcg                            >  1:5708441/1‑96 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATGAgaaaagatcg                            >  1:5040662/1‑96 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATCAgaaaagatcg                            >  1:5651932/1‑96 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATAAgaaagatcgg                            >  1:2005248/1‑94 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATAAgaaaagatcg                            >  1:1566155/1‑96 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AAACAgaagatcgga                            >  1:3850094/1‑93 (MQ=255)
                                   gATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAA‑AAATAATTAgatcggaag                             >  1:3158834/1‑93 (MQ=255)
                                   aaTCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATAAgaaaagatcg                            >  1:3505679/2‑96 (MQ=255)
                                    ctgcTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagatcgg                           >  1:857664/4‑95 (MQ=255)
                                    ctCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATCAgaaaagatcgg                           >  1:5395360/2‑95 (MQ=255)
                                    acagggaagaagaaGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagatcgg                           >  1:2932683/6‑95 (MQ=255)
                                    aTCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagatcgg                           >  1:910722/1‑95 (MQ=255)
                                    aTCCGGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagatcgg                           >  1:1264181/1‑95 (MQ=255)
                                     tCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAG‑AATTAgatcggaagagc                          >  1:5670830/1‑90 (MQ=255)
                                     cccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATCAgaaaagatcgga                          >  1:4277925/2‑94 (MQ=255)
                                      gcTGAAGAAGAAGAGCGTTTAAGAAAACGACATGATGAACGTAAACGTCGTAAAGAAGAA‑CAAGACAGAAAGCTTAAAG‑AAA‑AATTAgaaagagttcgga                         >  1:3986586/2‑91 (MQ=255)
                                      gcTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagcgaaaag                         >  1:4697377/2‑100 (MQ=255)
                                      ccTGAAGGAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagatcggaa                         >  1:3258723/1‑93 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTGgaaaagatcggaa                         >  1:5706614/1‑93 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagatcggaa                         >  1:272216/1‑93 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagatcggaa                         >  1:1635521/1‑93 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATCAgaaagatcggaag                         >  1:2630641/1‑91 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATCAgaaaagatcggaa                         >  1:71096/1‑93 (MQ=255)
                                      ccTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AAGTAgaaagatcggaag                         >  1:1692982/1‑91 (MQ=255)
                                        tGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagatcggaaga                       >  1:4849218/1‑91 (MQ=255)
                                        tGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAgaaaagatcggaaga                       >  1:2203757/1‑91 (MQ=255)
                                        tGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATGAgaaaagatcggaaga                       >  1:4481725/1‑91 (MQ=255)
                                        tGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATCAgaaaagatcggaaga                       >  1:3488720/1‑91 (MQ=255)
                                                             taaaCGACATGAAGAACGTAAACGTCGAAAAGAAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAGAAAAGCGAAAAGCACAACAATAAAGCCTGATGgca  >  1:4158885/2‑100 (MQ=255)
                                                                                             |                                                                      
TCGAAAAAGTTTCTCAACAATTAAAGAAACCACGTGATCCTGAAGAAGAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGTAGAA‑CAAGATAGAAAGCTTAAAG‑AAA‑AATTAGAAAAGCGAAAAGCACAACAATAAAGCCTGATGGCA  >  NC_007793/650058‑650218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑