Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 618,247 T→C D1297D (GAT→GAC)  SAUSA300_RS02920 → serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793618,2470TC81.8% 14.8 / ‑3.0 11D1297D (GAT→GAC) SAUSA300_RS02920serine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (9/0);  total (11/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGT  >  NC_007793/618152‑618325
                                                                                               |                                                                              
tCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGGGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCg                                                                            >  1:318079/1‑100 (MQ=255)
 cAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCga                                                                           >  1:1986948/1‑100 (MQ=255)
 cAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCga                                                                           >  1:2110561/1‑100 (MQ=255)
 cAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCga                                                                           >  1:4780889/1‑100 (MQ=255)
 cAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACCCAGATAGCGACTCAGACTCAGACAGCga                                                                           >  1:4696640/1‑100 (MQ=255)
 cAGACAGCGACTCAGACTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCga                                                                           >  1:1890229/1‑100 (MQ=255)
  aGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCgat                                                                          >  1:2854608/1‑100 (MQ=255)
            tCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGattcagactcag                                                                >  1:2201747/1‑100 (MQ=255)
             cAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAttcagactcaga                                                               >  1:485923/1‑100 (MQ=255)
             cAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAttcagactcaga                                                               >  1:596539/1‑100 (MQ=255)
                                                                          aGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGt  >  1:420570/1‑100 (MQ=11)
                                                                                               |                                                                              
TCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGT  >  NC_007793/618152‑618325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑