Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	617,707	A→C	G1117G (GGA→GGC)	SAUSA300_RS02920 →	serine‑aspartate repeat‑containing protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	617,707	0	A	C	100.0%	90.9 / NA	44	G1117G (GGA→GGC)	SAUSA300_RS02920	serine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (41/3);  total (41/3)

AACAGGTACAAATACAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGATACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGAT  >  NC_007793/617611‑617803                                                                                                 |                                                                                                 caCAGGTACAAATACAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCtac                                                                                               >  1:1043912/2‑100 (MQ=17) aaCAGGTACAAATACAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCtac                                                                                               >  1:1593709/1‑100 (MQ=17)         ctcatgcAACTGAAGATGATAAAGATGCAGATGGGGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACgaaga                                                                                       >  1:4768464/7‑100 (MQ=11)               cAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAAc                                                                                 >  1:3044221/1‑100 (MQ=17)                aaCTGAAGATGACAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACa                                                                                >  1:656923/1‑100 (MQ=12)                 ccTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACAt                                                                               >  1:4540425/2‑100 (MQ=17)                             aaaGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACt                                                                   >  1:4395388/1‑100 (MQ=17)                              taGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTc                                                                  >  1:1506715/2‑100 (MQ=17)                               aGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGTGACtaa                                                                 >  1:774119/1‑98 (MQ=255)                               aGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCa                                                                 >  1:4096768/1‑100 (MQ=17)                                tctGCAGATGGGGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAg                                                                >  1:1730024/3‑100 (MQ=255)                                 gtGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGcctcagatc                                                               >  1:2905490/2‑92 (MQ=11)                                 gtGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGa                                                               >  1:3145440/2‑100 (MQ=17)                                 gtGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCCACTACGAAGAAGAAACATCAGATAGCGACtagcg                                                               >  1:1145407/2‑96 (MQ=11)                                 ctGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGa                                                               >  1:3225418/2‑100 (MQ=17)                                 aggCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGAC‑CAGAt                                                              >  1:209434/3‑100 (MQ=255)                                  tGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACACAGAt                                                              >  1:4646707/1‑100 (MQ=12)                                  cgCAGATGGTGGCGTAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACCCAGAt                                                              >  1:1694936/2‑100 (MQ=255)                                   gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGGAGAAACATCAGATAGCGACCCAGAtc                                                             >  1:3383628/1‑99 (MQ=255)                                   gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGAcagatcgg                                                             >  1:2074864/1‑93 (MQ=255)                                   gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGact                                                             >  1:541775/1‑98 (MQ=17)                                   gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGAtt                                                             >  1:5476262/1‑100 (MQ=17)                                   gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGAtt                                                             >  1:5046634/1‑100 (MQ=17)                                   gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGAtt                                                             >  1:362768/1‑100 (MQ=17)                                   gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGAtt                                                             >  1:5214912/1‑100 (MQ=17)                                   gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGAtt                                                             >  1:4263623/1‑100 (MQ=17)                                   gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGAAACAGAtc                                                             >  1:2946004/1‑99 (MQ=255)                                     aGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCTACTCAGAtcgg                                                           >  1:1738327/1‑97 (MQ=2)                                     aGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACACAGAtcgg                                                           >  1:3462536/1‑97 (MQ=2)                                       gtggtggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGa                                                         >  1:2799266/2‑100 (MQ=17)                                        tggtggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGAc                                                        >  1:2318295/1‑100 (MQ=17)                                        gtgtggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGAc                                                        >  1:654679/3‑100 (MQ=17)                                        atgtggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGAc                                                        >  1:4457314/3‑100 (MQ=17)                                         cgtggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACa                                                       >  1:5722453/2‑100 (MQ=17)                                         cctggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACa                                                       >  1:364305/3‑100 (MQ=17)                                          ctggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAg                                                      >  1:316547/2‑100 (MQ=17)                                            ggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCg                                                    >  1:2002881/1‑100 (MQ=17)                                            ggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCg                                                    >  1:5576609/1‑100 (MQ=17)                                                caGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTc                                                >  1:5017111/2‑100 (MQ=25)                                                                  cGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGagactc                              <  1:5107886/100‑1 (MQ=255)                                                                                 acacaCTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGtaa               >  1:3846755/2‑98 (MQ=255)                                                                                    cacTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTc            <  1:1813828/100‑1 (MQ=255)                                                                                            aaTGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCgg    >  1:149088/1‑100 (MQ=255)                                                                                              tGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGTCTCAGATAGTGATTCAGACTCGGAt  <  1:4829314/100‑1 (MQ=255)                                                                                                 |                                                                                                 AACAGGTACAAATACAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGATACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGAT  >  NC_007793/617611‑617803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑