| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 613,318 | T→A | S724S (TCT→TCA) | SAUSA300_RS02915 → | serine‑aspartate repeat‑containing protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 613,318 | 0 | T | A | 95.5% | 52.4 / ‑4.0 | 22 | S724S (TCT→TCA) | SAUSA300_RS02915 | serine‑aspartate repeat‑containing protein C |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (18/3); total (18/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
GGTGGCGAAGTTGATGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGAT > NC_007793/613223‑613405 | ggtggCGAAGTTGATGTAACAATTACGGATCATGATGATTTCACACTTGATGATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACa > 1:4081025/1‑100 (MQ=255) tggCGAAGTTGATGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGc > 1:5254965/1‑100 (MQ=25) tggCGAAGTTGATGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGc > 1:2189437/1‑100 (MQ=25) atgatgATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACtcagatagcgactcagattc < 1:3748257/100‑1 (MQ=255) ttCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGAGACTCAGATTCAGACAGCGATTCAGACtcagatagcgactaagatcggaagagca > 1:2168454/1‑90 (MQ=17) aacacaCTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACtcagatagcgactcagattcagatagcg > 1:4481307/3‑100 (MQ=255) tGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACtcagatagcgactcagattcagatagcgactcagat > 1:3421765/1‑100 (MQ=255) aTAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATGCAGACTCagatagcgactcagattcagatagcgactcagattc < 1:411001/100‑1 (MQ=255) tAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAgatagcgactcagattcagatagcgactcagattca > 1:1082179/1‑100 (MQ=255) aaTGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGatagcgactcagattcaggtagcgactcagattcag > 1:3172738/1‑100 (MQ=255) ctGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAtagcgactcagattcagatagcgactcagattcaga > 1:5202486/2‑100 (MQ=255) aTGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAtagcgactcagattcagatagcgactcagattcaga > 1:2883956/1‑100 (MQ=255) ctactaCGAAGAAGAAACATCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAgattcagacagc < 1:3488764/100‑1 (MQ=255) actacGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAgattcagacagcga > 1:2863923/1‑100 (MQ=255) actacGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAgattcagacagcga > 1:1922213/1‑100 (MQ=255) actacGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAgattcagacagcga > 1:1693448/1‑100 (MQ=255) acGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGCCTCAGATAGCGACTCAGATTCAGATAGCGACTCAgattcagacagcgattc < 1:3085115/100‑1 (MQ=255) gaagagcacacCAGACAGTGATTCAGATTCAGACAGCGACTCAGAATCAGATAGCGACTCAGAATCAGATAGTGAGTCAgattcagacagtgactcggac > 1:539548/8‑100 (MQ=17) gaaCATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGAttcagacagcgattcagacagcga > 1:2749504/2‑100 (MQ=255) cAGACAGTGACTCGGACTCAGACAGTGATTCAGACTCAGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACAGCGATT‑AGATAGAgacccagat > 1:2138497/1‑99 (MQ=17) aaGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCgactcaga > 1:2382891/2‑100 (MQ=255) cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGAt > 1:1109381/1‑100 (MQ=255) | GGTGGCGAAGTTGATGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGAT > NC_007793/613223‑613405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |