Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	46,390	G→A	V377V (GTC→GTT)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	46,390	0	G	A	100.0%	58.4 / NA	21	V377V (GTC→GTT)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/20);  total (1/20)

ATTTATTTTGATTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTGATTTTCTTGATTGACGTGTGTTACTACGCGTTGAATGACTTTATCACTTTTGACTATTTCAAGTATTTGCTTCATCACATAATCTTCAATCACATCAGCTCTTACACT  >  NC_007793/46300‑46485                                                                                           |                                                                                                atttagttTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAAcacgtgct                                                                                        <  1:4463483/100‑8 (MQ=255)   ttagttTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCtac                                                                                      <  1:963282/100‑1 (MQ=255)   ttagttTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCtac                                                                                      <  1:215338/100‑1 (MQ=255)           gtttGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGtt                                                                              <  1:1758007/99‑1 (MQ=255)            tttGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTg                                                                             <  1:5337903/100‑1 (MQ=255)            tttGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTg                                                                             <  1:2318032/100‑1 (MQ=255)             ttGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTgg                                                                            <  1:4885458/100‑2 (MQ=255)             ttGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGa                                                                            <  1:1280373/100‑1 (MQ=255)                tACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGAATg                                                                         <  1:63052/100‑1 (MQ=255)                  cTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGATACAACGCGTTGAATgca                                                                       <  1:5226855/100‑3 (MQ=255)                   ttCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGAATggtg                                                                      <  1:3457821/100‑4 (MQ=255)                    tcatcaTATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGAATGaggt                                                                     <  1:1265480/100‑4 (MQ=255)                             tgttgttgCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGAATGACTTTATCACtta                                                            <  1:1492886/100‑2 (MQ=255)                                  ttgCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGAATGACTTTATCACTTTTGACt                                                       <  1:208586/100‑1 (MQ=255)                                       tataAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGAATGACTTTATCACTTTTGACTATTTc                                                  >  1:961419/1‑100 (MQ=255)                                                         gaAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGAATGACTTTATCACTTTTGACTATTTCGAGTATTTGCTTCATtac                                <  1:5461095/100‑1 (MQ=255)                                                                        cAACTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGAATGACTTTATCACTTTTGACTATTTCGAGTATTTGCTTCATTACATAATCTTCAATcac                 <  1:4176767/100‑1 (MQ=255)                                                                          aCTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGAATGACTTTATCACTTTTGACTATTTCGAGTATTTGCTTCATTACATAATCTTCAATcacca               <  1:210142/100‑3 (MQ=255)                                                                           cTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGAATGACTTTATCACTTTTGACTATTTCGAGTATTTGCTTCATTACATAATCTTCAATCACATc              <  1:3453916/100‑1 (MQ=255)                                                                           cTTTATTTTCTTGATTAACACGTGCTACAACGCGTTGAATGACTTTATCACTTTTGACTATTTCGAGTATTTGCTTCATTACATAATCTTCAATCACATc              <  1:3692448/100‑1 (MQ=255)                                                                           cTTTATTCTCTTGATTAACACGTGCTACAACGCGTTGAATGACTTTATCACTTTTGACTATTTCGAGTATTTGCTTCATTACATAATCTTCAATCACATc              <  1:5059780/100‑1 (MQ=255)                                                                                       gattgaCACGTGCTACAACGCGTTGAATGACTTTATCACTTTTGACTATTTCGAGTATTTGCTTCATTACATAATCTTCAATCACATCAGCTCTTacaca  <  1:5671130/100‑2 (MQ=255)                                                                                           |                                                                                                ATTTATTTTGATTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTGATTTTCTTGATTGACGTGTGTTACTACGCGTTGAATGACTTTATCACTTTTGACTATTTCAAGTATTTGCTTCATCACATAATCTTCAATCACATCAGCTCTTACACT  >  NC_007793/46300‑46485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑