Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 601,428 C→T P147S (CCT→TCT)  SAUSA300_RS02865 → Molecular chaperone Hsp31 and glyoxalase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793601,4280CT100.0% 634.6 / NA 215P147S (CCT→TCT) SAUSA300_RS02865Molecular chaperone Hsp31 and glyoxalase 3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (207/8);  total (207/8)

Only 100 of 215 total aligned reads displayed.

ACTGAAGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGACCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTCAACAAACATTAGATTGG  >  NC_007793/601332‑601523
                                                                                                 |                                                                                               
aCTGAAGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTg                                                                                              <  1:1031489/100‑1 (MQ=255)
aCTGAAGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTg                                                                                              >  1:2820112/1‑100 (MQ=255)
  tGAAGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGAt                                                                                            >  1:1253224/1‑100 (MQ=255)
  aaggtgcGAGGCAGTTATAGGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGAt                                                                                            >  1:900731/7‑100 (MQ=255)
   gAAGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGAtt                                                                                           >  1:5220207/1‑100 (MQ=255)
   gAAGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGAtt                                                                                           >  1:2708780/1‑100 (MQ=255)
   gAAGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGAtt                                                                                           >  1:1928531/1‑100 (MQ=255)
   ctaGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGAtt                                                                                           >  1:1046574/3‑100 (MQ=255)
   ctaGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGAtt                                                                                           >  1:1290958/3‑100 (MQ=255)
   ataGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGAtt                                                                                           >  1:3718528/3‑100 (MQ=255)
    taGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTc                                                                                          >  1:4653898/2‑100 (MQ=255)
    caGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTc                                                                                          >  1:1391569/2‑100 (MQ=255)
    aaGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTc                                                                                          >  1:3900186/1‑100 (MQ=255)
    aaGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTc                                                                                          >  1:795073/1‑100 (MQ=255)
    aaGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTc                                                                                          >  1:4629193/1‑100 (MQ=255)
    aaGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTc                                                                                          >  1:3550302/1‑100 (MQ=255)
     cgACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCa                                                                                         >  1:1494939/2‑100 (MQ=255)
     atgcGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCa                                                                                         >  1:728909/4‑100 (MQ=255)
     aGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAAAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCa                                                                                         >  1:4763576/1‑100 (MQ=255)
      ctcGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAg                                                                                        >  1:2073543/3‑100 (MQ=255)
      ctcGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAg                                                                                        >  1:4216482/3‑100 (MQ=255)
       ccGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGa                                                                                       >  1:4904069/2‑100 (MQ=255)
       ccGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGa                                                                                       >  1:4022251/2‑100 (MQ=255)
       ccGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGa                                                                                       >  1:1339910/2‑100 (MQ=255)
       cagAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGa                                                                                       >  1:1991132/3‑100 (MQ=255)
       aCGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGa                                                                                       >  1:3738948/1‑100 (MQ=255)
       aCGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGa                                                                                       >  1:4481920/1‑100 (MQ=255)
       aCGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGa                                                                                       >  1:2522461/1‑100 (MQ=255)
        tcagggggTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGAc                                                                                      >  1:300694/8‑100 (MQ=255)
        cGAGGCAGTTATAAGTACTTGTAATAAATTGAAAGAAGAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGAc                                                                                      >  1:2625117/1‑100 (MQ=255)
        cGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGAc                                                                                      >  1:5345230/1‑100 (MQ=255)
        cGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGAc                                                                                      >  1:1320564/1‑100 (MQ=255)
        cGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGAc                                                                                      >  1:3817278/1‑100 (MQ=255)
        cGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGAc                                                                                      >  1:361465/1‑100 (MQ=255)
        cGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGAc                                                                                      >  1:5076131/1‑100 (MQ=255)
        cGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGAc                                                                                      >  1:796536/1‑100 (MQ=255)
        cGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAAATAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGAc                                                                                      >  1:3236524/1‑100 (MQ=255)
        agAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGAc                                                                                      >  1:3457014/2‑100 (MQ=255)
         gAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACt                                                                                     >  1:5714345/1‑100 (MQ=255)
         gAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACt                                                                                     >  1:1700547/1‑100 (MQ=255)
         gAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACt                                                                                     >  1:47395/1‑100 (MQ=255)
         caGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACt                                                                                     >  1:5518586/2‑100 (MQ=255)
          aGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTa                                                                                    >  1:5650436/1‑100 (MQ=255)
          aGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTa                                                                                    >  1:1230740/1‑100 (MQ=255)
          aGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGAATa                                                                                    >  1:5783753/1‑100 (MQ=255)
             cAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTAttt                                                                                 >  1:5628370/1‑100 (MQ=255)
                    aaGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCCAAAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGt                                                                           >  1:276110/1‑100 (MQ=255)
                    aaGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTc                                                                          >  1:2027190/1‑100 (MQ=255)
                           gctaataaATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATcc                                                                   >  1:4151222/3‑100 (MQ=255)
                                                  aaaaCAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAgttgtt                                            >  1:1556878/1‑100 (MQ=255)
                                                  aaaaCAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAgttgtt                                            >  1:292524/1‑100 (MQ=255)
                                                   tggcAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGttgttg                                           >  1:568028/4‑100 (MQ=255)
                                                   aaaCAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAGTTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGttgttg                                           >  1:4288586/1‑100 (MQ=255)
                                                     gtggCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGt                                         >  1:5035043/4‑100 (MQ=255)
                                                     ctaGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGt                                         >  1:2983540/3‑100 (MQ=255)
                                                     ccAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGt                                         >  1:5581037/2‑100 (MQ=255)
                                                     aCAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGt                                         >  1:5132694/1‑100 (MQ=255)
                                                     aCAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGt                                         >  1:4578166/1‑100 (MQ=255)
                                                      taGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTa                                        >  1:2890218/2‑100 (MQ=255)
                                                      taGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTa                                        >  1:576812/2‑100 (MQ=255)
                                                      cAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTa                                        >  1:3563079/1‑100 (MQ=255)
                                                      cAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATCAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTa                                        >  1:1598581/1‑100 (MQ=255)
                                                       aGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTAt                                       >  1:335658/1‑100 (MQ=255)
                                                                   ttAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTg                            >  1:4067822/1‑100 (MQ=255)
                                                                   ttAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTg                            >  1:1893620/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:754392/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:3946960/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:2885402/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:5234973/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:3211112/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:3272310/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:4802871/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:4538348/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:4329122/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:4267672/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:2471200/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:2528201/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAACTGa                           >  1:2739383/1‑100 (MQ=255)
                                                                    tAGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTACTGAAAgatc                           >  1:4865558/1‑97 (MQ=255)
                                                                    aaGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGa                           >  1:2440970/2‑100 (MQ=255)
                                                                     aGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAg                          >  1:5160043/1‑100 (MQ=255)
                                                                     aGCAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAg                          >  1:538895/1‑100 (MQ=255)
                                                                       cAGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGa                        >  1:3660503/1‑100 (MQ=255)
                                                                        tgctgtgATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGAc                       >  1:3433541/4‑100 (MQ=255)
                                                                        aGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGAc                       >  1:4116555/1‑100 (MQ=255)
                                                                        aGATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGGCTTTATCCCAGGCGGCCATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGAc                       >  1:2671307/1‑100 (MQ=255)
                                                                         gATGTGATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAgatcggaag                      >  1:2250911/1‑92 (MQ=255)
                                                                          gtgtgATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGt                     >  1:5566622/2‑100 (MQ=255)
                                                                           tgtgATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAgatcggaagag                    >  1:5551651/1‑90 (MQ=255)
                                                                           tgtgATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGtt                    >  1:3867055/1‑100 (MQ=255)
                                                                           gctgATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGtt                    >  1:5279205/3‑100 (MQ=255)
                                                                            gtgATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTc                   >  1:385245/1‑100 (MQ=255)
                                                                            gtgATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTc                   >  1:3089008/1‑100 (MQ=255)
                                                                            gtgATTAAAAATGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAAGatcgga                   >  1:695733/1‑95 (MQ=255)
                                                                                    aaaTGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTCaacaaaca           >  1:1815774/1‑100 (MQ=255)
                                                                                     aaTGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTCAACAAACAt          >  1:671262/1‑100 (MQ=255)
                                                                                      aTGAATTAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTCAACAAACAtt         <  1:1202243/100‑1 (MQ=255)
                                                                                      aTGAAATAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGTGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGCCGTTCAACAAACAtt         <  1:3284559/100‑1 (MQ=255)
                                                                                            tAGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGCATTTCTGAAAGTGAGGACGTTCAACAAACATTAGATTg   >  1:2274439/1‑100 (MQ=255)
                                                                                             aGGATCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTCAACAAACATTAGATTgg  >  1:1157075/1‑100 (MQ=255)
                                                                                                 |                                                                                               
ACTGAAGACGAGGCAGTTATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCC‑AAAAAAATTAGCAGATGTGATTAAAAATGAATTAGGACCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACATGCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTCAACAAACATTAGATTGG  >  NC_007793/601332‑601523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑