Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	591,811	A→G	H857R (CAT→CGT)	SAUSA300_RS02825 →	DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	591,811	0	A	G	100.0%	173.7 / NA	64	H857R (CAT→CGT)	SAUSA300_RS02825	DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (63/1);  total (63/1)

GATAGAGGTTTATTAGTTTCTGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCATCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGAAAATTACAGATGCTGGTATTGAACAA  >  NC_007793/591714‑591902                                                                                                  |                                                                                            gATAGAGGTTTATTAGTTTCTGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATtcgtc                                                                                           >  1:78080/1‑100 (MQ=255) gATAGAGGTTTATTAGTTTCTGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATtcgtc                                                                                           >  1:443747/1‑100 (MQ=255) gATAGAGGTTTATTAGTTTCTGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATtcgtc                                                                                           >  1:4448951/1‑100 (MQ=255) gATAGAGGTTTATTAGTTTCTGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATtcgtc                                                                                           >  1:3669694/1‑100 (MQ=255) gATAGAGGTTTATTAGTTTCTGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATtcgtc                                                                                           >  1:5033037/1‑100 (MQ=255)                    cTGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAaat                                                                        >  1:1481693/1‑100 (MQ=255)                     tGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAaata                                                                       >  1:4111871/1‑100 (MQ=255)                     tGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAaata                                                                       >  1:3327873/1‑100 (MQ=255)                      gATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAaataa                                                                      >  1:4436273/1‑100 (MQ=255)                      gATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAaataa                                                                      >  1:1819624/1‑100 (MQ=255)                      gATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAaataa                                                                      >  1:2907255/1‑100 (MQ=255)                      gATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAaataa                                                                      >  1:2742605/1‑100 (MQ=255)                             gtgcagGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCg                                                               >  1:268678/5‑100 (MQ=255)                              agaagGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGt                                                              >  1:4151387/1‑100 (MQ=255)                               caagGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTc                                                             >  1:2900574/2‑100 (MQ=255)                                acggTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTcc                                                            >  1:962047/3‑100 (MQ=255)                                accgcttggAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATCCGTcc                                                            <  1:4015317/92‑1 (MQ=255)                                aagGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTcc                                                            >  1:5336812/1‑100 (MQ=255)                                aagGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTcc                                                            >  1:4081854/1‑100 (MQ=255)                                aagGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTcc                                                            >  1:3759720/1‑100 (MQ=255)                                aagGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTcc                                                            >  1:2791002/1‑100 (MQ=255)                                aagGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTcc                                                            >  1:4733920/1‑100 (MQ=255)                                 agGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCt                                                           >  1:2328117/1‑100 (MQ=255)                                 agGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCt                                                           >  1:495660/1‑100 (MQ=255)                                  tatACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtg                                                          >  1:2002575/3‑100 (MQ=255)                                  ggTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtg                                                          >  1:4131762/1‑100 (MQ=255)                                  ggTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtg                                                          >  1:1610816/1‑100 (MQ=255)                                  ggTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtg                                                          >  1:4832895/1‑100 (MQ=255)                                  ggTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtg                                                          >  1:5458064/1‑100 (MQ=255)                                  ggTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtg                                                          >  1:2329856/1‑100 (MQ=255)                                   gTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtga                                                         >  1:2045413/1‑100 (MQ=255)                                     aCAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtgatg                                                       >  1:3513796/1‑100 (MQ=255)                                     aCAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtgatg                                                       >  1:644013/1‑100 (MQ=255)                                     aCAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtgatg                                                       >  1:4277017/1‑100 (MQ=255)                                     aCAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtgatg                                                       >  1:1316143/1‑100 (MQ=255)                                     aCAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtgatg                                                       >  1:1864242/1‑100 (MQ=255)                                      cAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCtgatga                                                      >  1:3974076/1‑100 (MQ=255)                                                 taaCCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTacac                                           >  1:4107384/2‑100 (MQ=255)                                                 gAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTacac                                           >  1:3384212/1‑100 (MQ=255)                                                 gAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTacac                                           >  1:3431600/1‑100 (MQ=255)                                                 gAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTacac                                           >  1:5523726/1‑100 (MQ=255)                                                 gAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTacac                                           >  1:2768576/1‑100 (MQ=255)                                                 caaCCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTacac                                           >  1:2335335/2‑100 (MQ=255)                                                            cGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCt                                >  1:2723390/1‑100 (MQ=255)                                                            cGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCt                                >  1:2653491/1‑100 (MQ=255)                                                            acgtgtgaTTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCt                                >  1:2456662/8‑100 (MQ=255)                                                              taCGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTaa                              >  1:3827951/2‑100 (MQ=255)                                                              taCGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTaa                              >  1:4899278/2‑100 (MQ=255)                                                              caCGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGaagatcggaa                              >  1:3626494/2‑92 (MQ=255)                                                              caCGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTaa                              >  1:5576559/2‑100 (MQ=255)                                                              aaCGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAGTTAATTACACCTGAAATTGCTaa                              >  1:3844514/1‑100 (MQ=255)                                                              aaCGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTaa                              >  1:231302/1‑100 (MQ=255)                                                              aaCGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTaa                              >  1:3451440/1‑100 (MQ=255)                                                              aaCGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTaa                              >  1:3118002/1‑100 (MQ=255)                                                               aCGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTAATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAg                             >  1:2450605/1‑100 (MQ=255)                                                                tgTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGa                            >  1:11079/2‑100 (MQ=255)                                                                 ctATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGaa                           >  1:2868526/2‑100 (MQ=255)                                                                  tATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGatc                          >  1:5261735/1‑98 (MQ=255)                                                                  tATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGaaa                          >  1:1193866/1‑100 (MQ=255)                                                                     tGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATACTCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGAAAAtt                       >  1:5454136/1‑100 (MQ=255)                                                                      caaGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAACTGCTAAGatcggaa                      >  1:3695972/2‑94 (MQ=255)                                                                      aaaGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGAAAATTa                      >  1:4986695/2‑100 (MQ=255)                                                                      aaaGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGAAAATTa                      >  1:1970471/2‑100 (MQ=255)                                                                       aaGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGAAAATTAc                     >  1:138249/1‑100 (MQ=255)                                                                        aGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGAAAATTACa                    >  1:4303419/1‑100 (MQ=255)                                                                           agcttATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGAAAATTACAGAt                 >  1:803648/4‑100 (MQ=255)                                                                            cGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGAAAATTACAGATg                >  1:269583/1‑100 (MQ=255)                                                                                     aaaGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCCGATGAATTAATTACACCTGAAATTGCTAAGAAAATTACAGATGCTGGTATTg       >  1:4703346/1‑100 (MQ=255)                                                                                          aaCAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGAAAATTACAGATGCTGGTATTGAAcca  >  1:2102968/1‑98 (MQ=255)                                                                                                  |                                                                                            GATAGAGGTTTATTAGTTTCTGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCATCATCCTGAAACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGAAAATTACAGATGCTGGTATTGAACAA  >  NC_007793/591714‑591902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑