Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	578,108	T→C	Y341H (TAT→CAT)	SAUSA300_RS02760 →	cysteine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	578,108	0	T	C	100.0%	163.5 / NA	62	Y341H (TAT→CAT)	SAUSA300_RS02760	cysteine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (62/0);  total (62/0)

AGAATCAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACATATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTATGATTTAG  >  NC_007793/578011‑578204                                                                                                  |                                                                                                 aGAATCAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACacat                                                                                                >  1:744335/1‑100 (MQ=255) aGAATCAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACacat                                                                                                >  1:1344021/1‑100 (MQ=255)   caTCAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACacatat                                                                                              >  1:3308740/2‑100 (MQ=255)     tCAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTg                                                                                            >  1:1024092/1‑100 (MQ=255)     tCAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTg                                                                                            >  1:2728499/1‑100 (MQ=255)     acAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATGAATTAATTGAAGAGCGCGCACAAATTTCTACTAATATTGAAAATCAACAGACACATATTg                                                                                            >  1:3225136/2‑100 (MQ=255)     acAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTg                                                                                            >  1:2903832/2‑100 (MQ=255)      cAGCACGTAGTGGACTAGGGCGTATTCGCAATAGTTATCAATTTATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:2139092/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGCGCGTATTCGCAATAGTTATCATTTAATTGCAGAGCGCGCACAAATTGCTACTAATATTGCAAATCAACAGACACTTATTGa                                                                                           >  1:3881990/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAGGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:1508740/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACagatcggaa                                                                                           >  1:1380378/1‑95 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:2012688/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:3333909/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:2829886/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:274808/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:2266539/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:2162101/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:4811623/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:689761/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:1769660/1‑100 (MQ=255)      cAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGa                                                                                           >  1:1736826/1‑100 (MQ=255)        gCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATc                                                                                         >  1:1795387/1‑100 (MQ=255)        acACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATc                                                                                         >  1:4454705/2‑100 (MQ=255)          aCGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCaa                                                                                       >  1:1846380/1‑100 (MQ=255)           cGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACagatcggaagagca                                                                                      >  1:496008/1‑90 (MQ=255)           cGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCaaa                                                                                      >  1:1944508/1‑100 (MQ=255)           cGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCaaa                                                                                      >  1:733802/1‑100 (MQ=255)            gtgtgggACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAAt                                                                                     >  1:3225748/6‑100 (MQ=255)             tAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAAtt                                                                                    >  1:2012094/1‑100 (MQ=255)              aGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTg                                                                                   >  1:3675752/1‑100 (MQ=255)              aGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTg                                                                                   >  1:170384/1‑100 (MQ=255)              aGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTg                                                                                   >  1:3507078/1‑100 (MQ=255)              aGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTg                                                                                   >  1:3475199/1‑100 (MQ=255)              aGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTg                                                                                   >  1:4483494/1‑100 (MQ=255)               atGGACTAAAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGa                                                                                  >  1:15901/2‑100 (MQ=255)                tGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGAt                                                                                 >  1:2110995/1‑100 (MQ=255)                ctgACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAAAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGAt                                                                                 >  1:2353455/3‑100 (MQ=255)                 ggACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATg                                                                                >  1:3077343/1‑100 (MQ=255)                 ggACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATg                                                                                >  1:5451795/1‑100 (MQ=255)                 ggACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATg                                                                                >  1:616892/1‑100 (MQ=255)                 ggACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATg                                                                                >  1:1162681/1‑100 (MQ=255)                 ggACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATg                                                                                >  1:2184824/1‑100 (MQ=255)                 ggACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATg                                                                                >  1:2171352/1‑100 (MQ=255)                 ggACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATg                                                                                >  1:209436/1‑100 (MQ=255)                                  cAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGtttt                                                               >  1:2905088/1‑100 (MQ=255)                                     tAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGaa                                                            >  1:4948122/1‑100 (MQ=255)                                             agttaattGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTat                                                    >  1:4575524/3‑100 (MQ=255)                                                 aattGAAGAGCGAGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTatgaat                                                >  1:1238394/1‑100 (MQ=255)                                                     gAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAatgatg                                            >  1:1699145/1‑100 (MQ=255)                                                          gcgcgcACAAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGAtttt                                       >  1:3623924/1‑100 (MQ=255)                                                           ctgacccaAATTGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTa                                      >  1:1196581/7‑100 (MQ=255)                                                                  agcttGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGGTGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGc                               >  1:3148510/4‑100 (MQ=255)                                                                     ttGCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTaa                            >  1:5432594/1‑100 (MQ=255)                                                                       gCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATg                          >  1:3095401/1‑100 (MQ=255)                                                                       gCTACTAATATTGAAAATCAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATg                          >  1:1293247/1‑100 (MQ=255)                                                                          actaATATTGAAAATCTACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCaa                       >  1:4451579/1‑100 (MQ=255)                                                                                        tgaaCAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTAt         >  1:1732288/3‑100 (MQ=255)                                                                                        tCAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTAt         >  1:1407219/1‑100 (MQ=255)                                                                                         cAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTATg        >  1:3486566/1‑100 (MQ=255)                                                                                         cAACAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTATg        >  1:594070/1‑100 (MQ=255)                                                                                           aCAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTATGAt      >  1:1311848/1‑100 (MQ=255)                                                                                           aCAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTATGAt      >  1:1974645/1‑100 (MQ=255)                                                                                            cAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTATGAtt     >  1:3896761/1‑100 (MQ=255)                                                                                            cAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTATGAtt     >  1:5169992/1‑100 (MQ=255)                                                                                            cAGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTATGAtt     >  1:1023380/1‑100 (MQ=255)                                                                                             cgACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTATGAttt    >  1:668551/2‑100 (MQ=255)                                                                                             aGACACATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTATGAttt    >  1:5318710/1‑100 (MQ=255)                                                                                               acacATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTggaagatcggaa  >  1:906954/1‑90 (MQ=255)                                                                                                  |                                                                                                 AGAATCAGCACGTAGTGGACTAGAGCGTATTCGCAATAGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACATATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTTAATACAGCTAATGCAATTACAGCTTGGTATGATTTAG  >  NC_007793/578011‑578204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑