Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	552,065	T→C	N24N (AAT→AAC)	SAUSA300_RS02640 →	dihydropteroate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	552,065	0	T	C	100.0%	159.0 / NA	59	N24N (AAT→AAC)	SAUSA300_RS02640	dihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (59/0);  total (59/0)

CAATACATAATTAAAGGGTGGTTAACATGACTAAAACAAAAATTATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAATAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAACGAGACCCG  >  NC_007793/551968‑552153                                                                                                  |                                                                                         cAATACATAATTAAAGGGTGGTTAACATGACTAAAACAAAAATTATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTtaacaa                                                                                        >  1:1765463/1‑100 (MQ=255)   aTACATAATTAAAGGGTGGTTAACATGACTAAAAC‑AAAATTATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGt                                                                                     >  1:2730504/1‑100 (MQ=255)     aCATAATTAAAGGGTGGTTAACATGACTAAAACAAAAATTATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGtt                                                                                    >  1:4408630/1‑100 (MQ=255)            tAACGGGTGGTTAACATGACTAAAACAAAAATTATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAg                                                                             >  1:5661164/1‑100 (MQ=255)             aaaGGGTGGTTAACATGACTAAAACAAAAATTATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGc                                                                            >  1:975315/1‑100 (MQ=255)                    ggtTAACATGACTAAAACAAAAATTATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAAt                                                                     >  1:5405065/1‑100 (MQ=255)                             gACTAAAACAAAAATTATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGaaa                                                            >  1:807107/1‑100 (MQ=255)                                 aaaaCAAAAATTATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCa                                                        >  1:4840764/1‑100 (MQ=255)                                  aaaCAAAAATTATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCAt                                                       >  1:4876763/1‑100 (MQ=255)                                          cttATGGGCATATTAAACGTCACACCTGATTCATTCTCCGATGGTGGAAAATTTAACAATGTTGAATCAGCTATCAATAGAGTGAAAGCCATGAtcgctg                                               >  1:3441313/2‑95 (MQ=255)                                           ttATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGa                                              >  1:3420589/1‑100 (MQ=255)                                            tctGGGCATATTGAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGaa                                             >  1:3977694/3‑100 (MQ=255)                                             ctGGGCATATTAAACGTCACACCCGATTCATTCTCAGATGGAGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAg                                            >  1:2087489/2‑100 (MQ=255)                                              tGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAgg                                           >  1:112216/1‑100 (MQ=255)                                               gggCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAAGt                                          >  1:2283566/1‑100 (MQ=255)                                               ccttcatatAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGt                                          >  1:3522261/9‑100 (MQ=255)                                                ggCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTg                                         >  1:788149/1‑100 (MQ=255)                                                 gCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGc                                        >  1:4147080/1‑100 (MQ=255)                                                                       ttcattcTCAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGgt                  >  1:5558216/1‑100 (MQ=255)                                                                            tctcAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTc             >  1:2500152/1‑100 (MQ=255)                                                                            tctcAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTc             >  1:5466922/1‑100 (MQ=255)                                                                            tctcAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTc             >  1:5706804/1‑100 (MQ=255)                                                                            tctcAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTc             >  1:4477763/1‑100 (MQ=255)                                                                            tctcAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTc             >  1:1561342/1‑100 (MQ=255)                                                                            tctcAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTc             >  1:4283760/1‑100 (MQ=255)                                                                            tctcAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTc             >  1:371101/1‑100 (MQ=255)                                                                             ctcAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAGAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCa            >  1:144715/1‑100 (MQ=255)                                                                             ctcAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCa            >  1:3584157/1‑100 (MQ=255)                                                                             ctcAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCa            >  1:5346661/1‑100 (MQ=255)                                                                             cccAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCa            >  1:3868622/3‑100 (MQ=255)                                                                              tcAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCaa           >  1:2839103/1‑100 (MQ=255)                                                                               cAGATGGTGGCAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGCTAGATGAAGGTGCTGACATTATCGATGTTGGAGGTGTTTCAAc          >  1:182/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTTACATTATAGATGTTGGAGGTGTTTCAAc          >  1:3925309/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:5138802/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:1785266/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:729732/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:2375942/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:5645382/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:2714909/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:2831351/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:5318859/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:5220300/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:5215283/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:3284727/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:3313780/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:4386091/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:3849706/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:4150884/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:3627051/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:4220466/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGGGTTTCAAc          >  1:3939675/1‑100 (MQ=255)                                                                               cAGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGAGCTGACATTATAGATGTTGGAGGTGTTTCAAc          >  1:1857969/1‑100 (MQ=255)                                                                                tgctggtggAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAACg         >  1:4873019/4‑100 (MQ=255)                                                                                aGATGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAACg         >  1:4385687/1‑100 (MQ=255)                                                                                  aTGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAACgag       >  1:3216955/1‑100 (MQ=255)                                                                                  aTGGTGGAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAACgag       >  1:4499491/1‑100 (MQ=255)                                                                                   tggtggAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAACgaga      >  1:5162475/1‑100 (MQ=255)                                                                                    tgtggAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAACGAGAc     >  1:3993605/2‑100 (MQ=255)                                                                                    ggtggAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGCGTTTCAACGAGAc     >  1:5448203/1‑100 (MQ=255)                                                                                       ggAAAATTTAACAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAACGAGACCCg  >  1:2392945/1‑100 (MQ=255)                                                                                                  |                                                                                         CAATACATAATTAAAGGGTGGTTAACATGACTAAAACAAAAATTATGGGCATATTAAACGTCACACCTGATTCATTCTCAGATGGTGGAAAATTTAATAATGTTGAATCAGCTATAAATAGAGTGAAAGCCATGATAGATGAAGGTGCTGACATTATAGATGTTGGAGGTGTTTCAACGAGACCCG  >  NC_007793/551968‑552153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑